Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2071.G98.7% 255.8 / ‑3.5 77noncoding (80/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (34/42);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4606068‑4606332
                                                                                                                                            |                                                                                                                                
gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGa                                                                                                                                   <  1:1087270/140‑1 (MQ=255)
    aGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gg                                                                                                                            >  2:524246/1‑140 (MQ=255)
      aTAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggg                                                                                                                          <  2:208053/140‑1 (MQ=255)
        aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggggg                                                                                                                        >  2:50890/1‑140 (MQ=255)
        aaTTGGTGCGAGGGAGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gg‑gggggg                                                                                                                        >  2:652239/1‑140 (MQ=255)
             gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGA‑‑gg‑gggggggactt                                                                                                                   <  2:588719/140‑6 (MQ=255)
               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGa                                                                                                                 >  2:431379/1‑140 (MQ=255)
                cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGaa                                                                                                                >  1:518693/1‑140 (MQ=255)
                cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGaa                                                                                                                >  1:171028/1‑140 (MQ=255)
                   ggggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAAccc                                                                                                             <  2:1433324/140‑1 (MQ=255)
                     ggggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAAccccc                                                                                                           <  2:1482388/140‑1 (MQ=255)
                      aggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTTCTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCa                                                                                                          <  1:652239/139‑1 (MQ=255)
                          gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTc                                                                                                      <  1:50890/140‑1 (MQ=255)
                            cTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTcag                                                                                                    >  1:152223/1‑138 (MQ=255)
                             ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGt                                                                                                   <  1:431379/140‑1 (MQ=255)
                               gaccccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCa                                                                                                          <  2:150495/128‑1 (MQ=255)
                               gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCa                                                                                                          >  1:150495/1‑131 (MQ=255)
                               gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTaa                                                                                                 >  2:87179/1‑140 (MQ=255)
                               gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTCCGA‑‑GG‑CGGGGGACCTAGAACCCCCACGTCGGTaa                                                                                                 >  2:1353041/1‑140 (MQ=25)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAg                                                                                                >  2:689384/1‑140 (MQ=255)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAg                                                                                                <  1:1299043/140‑1 (MQ=255)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAg                                                                                                <  1:754002/140‑1 (MQ=255)
                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAg                                                                                                <  1:689384/140‑1 (MQ=255)
                                 aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGa                                                                                               <  2:737252/140‑1 (MQ=255)
                                    cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAaca                                                                                            >  2:1297003/1‑140 (MQ=255)
                                    cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCAGTAAGAaca                                                                                            <  2:152223/140‑1 (MQ=255)
                                      cACGTGCGTAAGGACACTAGCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  2:1183540/140‑1 (MQ=37)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  2:518693/140‑1 (MQ=255)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  2:1255775/140‑1 (MQ=255)
                                      cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACt                                                                                          <  1:376509/140‑1 (MQ=255)
                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                        <  1:429155/140‑1 (MQ=255)
                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                    <  1:524246/140‑1 (MQ=255)
                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                    >  1:1068543/1‑140 (MQ=255)
                                             gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                                                   >  1:124414/1‑140 (MQ=255)
                                                 ggACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                                               >  1:960577/1‑140 (MQ=255)
                                                   acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                             >  1:291247/1‑140 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                           >  1:530582/1‑140 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                           >  1:953559/1‑140 (MQ=255)
                                                     acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                           <  2:291247/140‑1 (MQ=255)
                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                         <  2:400553/140‑1 (MQ=255)
                                                         acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                       <  2:124414/140‑1 (MQ=255)
                                                            ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAATGCGTCtc                                                                   >  2:205991/1‑139 (MQ=255)
                                                             cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                   >  1:1080186/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                 >  1:206294/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                 >  2:765525/1‑140 (MQ=255)
                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                 >  2:309936/1‑140 (MQ=255)
                                                                aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                <  2:171028/140‑1 (MQ=255)
                                                                 aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCTTATTACTTTTATTGATATCGTCCCGCTGAGGGGGA‑‑GGCGGGGGGA‑CTTCAACTCCTACGCCAGAAATAATACTAACCCCAGAAGCAATCGCTGCTACCaa                                                               >  2:494065/1‑140 (MQ=12)
                                                                 aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                               <  2:1080186/140‑1 (MQ=255)
                                                                      gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                          <  1:660740/140‑1 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         <  1:309936/140‑1 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         >  1:972263/1‑140 (MQ=255)
                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         <  1:87179/140‑1 (MQ=255)
                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                     >  1:1045313/1‑140 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  1:1297003/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  2:1068543/140‑1 (MQ=255)
                                                                                 aaTTCCGCCACCTTCGCACAGTCATCTTACTATTTTTGATATCGACTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                               <  2:1025627/140‑1 (MQ=255)
                                                                                  aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                              <  1:1218763/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         >  1:930619/1‑125 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                           >  1:1020430/1‑139 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                           <  2:1020430/139‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          >  1:1152060/1‑140 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          <  1:1166906/140‑1 (MQ=255)
                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                          >  1:1350340/1‑140 (MQ=255)
                                                                                      cGCCACCTTCGCACACTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                         <  2:930619/125‑1 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                        <  2:1152060/140‑1 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                        >  2:916857/1‑140 (MQ=255)
                                                                                        ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                        >  2:535068/1‑140 (MQ=255)
                                                                                         cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGC‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGCCTACCAATTCCCCCACCTTCGCCCAGTGCGa                                       >  1:457071/1‑140 (MQ=21)
                                                                                         cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                       <  2:960577/140‑1 (MQ=255)
                                                                                          tccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  1:1353041/139‑1 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTAGCCCAGTGCGAg                                      >  2:346220/1‑140 (MQ=255)
                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCCGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                      <  2:1045313/140‑1 (MQ=255)
                                                                                                    aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                            <  2:530582/140‑1 (MQ=255)
                                                                                                    aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                            <  1:765525/140‑1 (MQ=255)
                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                          >  1:813317/1‑140 (MQ=255)
                                                                                                          cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGG‑GGGGGGA‑CTTGAACCCCCACg                                                                                                        >  2:641343/1‑59 (MQ=25)
                                                                                                          cTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGG‑GGGGGGA‑CTTGAACCCCCACg                                                                                                        <  1:641343/59‑1 (MQ=25)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                >  2:623103/1‑140 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                >  1:55102/1‑140 (MQ=255)
                                                                                                                ttttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                <  1:916857/140‑1 (MQ=255)
                                                                                                                 tttttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg               <  1:738784/140‑1 (MQ=255)
                                                                                                                    ttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa            >  2:33612/1‑140 (MQ=255)
                                                                                                                    ttGATATCGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa            <  2:206294/140‑1 (MQ=255)
                                                                                                                           cGCCTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg     >  2:739704/1‑140 (MQ=255)
                                                                                                                              cTCGTTTGGTGCGAG‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc  <  2:55102/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                
GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GG‑GGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAC  >  NC_000913/4606068‑4606332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑