Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,955	(C)5→6	coding (402/1374 nt)	fliI →	flagellum‑specific ATP synthase FliI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,950	1	.	C	100.0%	160.4 / NA	46	S133P (TCC→CCC)	fliI	flagellum‑specific ATP synthase FliI
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/22);  total (24/22)

AGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCT‑‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCG  >  NC_000913/2016811‑2017074                                                                                                                                             |                                                                                                                              aGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCt                                                                                                                                <  2:776030/140‑1 (MQ=255)                cgcgcgTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCCCGATacgac                                                                                                                 >  1:1226628/1‑140 (MQ=255)                cgcgcgTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATacgacg                                                                                                                >  2:1256934/1‑140 (MQ=255)                cgcgcgTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATacgacg                                                                                                                >  1:533281/1‑140 (MQ=255)                           aTGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTgc                                                                                                     <  2:980227/140‑1 (MQ=255)                           aTGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTgc                                                                                                     <  1:870423/140‑1 (MQ=255)                                 aaaaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGAt                                                                                               >  2:1162608/1‑140 (MQ=255)                                 aaaaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGAt                                                                                               >  1:949290/1‑140 (MQ=255)                                  aaaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGAtt                                                                                              >  1:17315/1‑140 (MQ=255)                                   aaCATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTa                                                                                             >  1:1301760/1‑140 (MQ=255)                                        ttCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCg                                                                                        <  2:1301760/140‑1 (MQ=255)                                               gAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTa                                                                                 <  1:1437001/140‑1 (MQ=255)                                                 aGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAAc                                                                               <  2:17315/140‑1 (MQ=255)                                                    gCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCg                                                                            <  1:1053558/140‑1 (MQ=255)                                                    gCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCg                                                                            <  1:446986/140‑1 (MQ=255)                                                      tGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGtt                                                                          <  2:9247/140‑1 (MQ=255)                                                      tGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGtt                                                                          >  1:12279/1‑140 (MQ=255)                                                      tGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGtt                                                                          <  1:1256934/140‑1 (MQ=255)                                                                    cAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGa                                                            <  1:1259001/140‑1 (MQ=255)                                                                            gCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACAt                                                    <  2:967546/140‑1 (MQ=255)                                                                               cGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACAt                                                    <  2:1287024/137‑1 (MQ=255)                                                                               cGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACAt                                                    >  1:1287024/1‑137 (MQ=255)                                                                                  tCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTCCCCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCt                                               <  2:1226628/140‑1 (MQ=255)                                                                                     ggCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAAc                                                      >  2:1287580/1‑129 (MQ=255)                                                                                     ggCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAAc                                                      <  1:1287580/129‑1 (MQ=255)                                                                                     ggCGTTATTAGGTCGCGTTCTGGACGGC‑GCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGaa                                                       >  1:353564/1‑127 (MQ=255)                                                                                     ggCGTTATTAGGTCGCGTTCTGGACGGC‑GCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGaa                                                       <  2:353564/127‑1 (MQ=255)                                                                                          tattaGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCgg                                      >  1:481435/1‑140 (MQ=255)                                                                                           attaGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGc                                     <  2:12279/140‑1 (MQ=255)                                                                                            ttaGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCg                                    >  1:179024/1‑140 (MQ=255)                                                                                            ttaGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCg                                    >  2:1359387/1‑140 (MQ=255)                                                                                            ttaGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCg                                    >  1:386073/1‑140 (MQ=255)                                                                                                gTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCg                                    >  1:1302047/1‑136 (MQ=255)                                                                                                gTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCg                                    <  2:1302047/136‑1 (MQ=255)                                                                                                    cgTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCca                            >  1:771271/1‑140 (MQ=255)                                                                                                    cgTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCca                            >  1:1022611/1‑140 (MQ=255)                                                                                                            aCGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGc                    <  2:481435/140‑1 (MQ=255)                                                                                                             cGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGcc                   <  2:1069726/140‑1 (MQ=255)                                                                                                             cGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGcc                   <  2:1022611/140‑1 (MQ=255)                                                                                                             cGGCAGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGcc                   <  1:1359387/140‑1 (MQ=255)                                                                                                                 aGCGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGc               >  1:574949/1‑140 (MQ=255)                                                                                                                   cGGTAAACCGCTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCtt             >  1:1326826/1‑140 (MQ=255)                                                                                                                             cTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGt        <  1:927596/135‑1 (MQ=255)                                                                                                                             cTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGt        >  2:927596/1‑135 (MQ=255)                                                                                                                             cTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGc   >  1:1432057/1‑140 (MQ=255)                                                                                                                             cTCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCA‑TTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCg  >  1:286270/1‑140 (MQ=255)                                                                                                                              tCGATGGCCTGCCCTC‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCg  >  1:1357075/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                              AGGTGTCCTGCCCGGCGCGCGTGTTTATGCCAAAAACATTTCGGCAGAAGGGCTGCAAAGCGGCAAGCAGTTGCCGCTCGGTCCGGCGTTATTAGGTCGCGTTCTGGACGGCAGCGGTAAACCGCTCGATGGCCTGCCCT‑‑CCCCCGATACGACGGAAACCGGTGCGCTGATTACCCCGCCATTTAACCCGTTGCAACGTACACCGATTGAACATGTGCTGGACACCGGCGTGCGCCCAATCAATGCCCTGCTTACCGTTGGGCG  >  NC_000913/2016811‑2017074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑