Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,698	G→A	G122G (GGC→GGT)	rnc ←	RNase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,698	0	G	A	100.0%	201.8 / NA	58	G122G (GGC→GGT)	rnc	RNase III
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/25);  total (33/25)

GGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACGCCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGCCAGC  >  NC_000913/2703562‑2703824                                                                                                                                         |                                                                                                                               ggCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACAcca                                                                                                                             <  2:1093013/140‑1 (MQ=255) ggCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACAcca                                                                                                                             <  2:333156/140‑1 (MQ=255)         tGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTaa                                                                                                                     >  2:1481578/1‑140 (MQ=255)                atTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCg                                                                                                              >  2:1188731/1‑140 (MQ=255)                 ttCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGa                                                                                                             <  2:112476/140‑1 (MQ=255)                 ttCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGa                                                                                                             >  1:1277172/1‑140 (MQ=255)                 ttCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGa                                                                                                             <  2:165328/140‑1 (MQ=255)                   cTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACg                                                                                                           <  2:1277172/140‑1 (MQ=255)                   cTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACg                                                                                                           >  2:585833/1‑140 (MQ=255)                     tGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGgt                                                                                                         >  1:591588/1‑140 (MQ=255)                     tGCAAGCGCGTTTTCGGATC‑TTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGgtg                                                                                                        >  2:476128/1‑140 (MQ=255)                       cAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATg                                                                                                                   >  1:1473899/1‑128 (MQ=255)                       cAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATg                                                                                                                   <  2:1473899/128‑1 (MQ=255)                           cgcgTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGc                                                                                                   >  2:1035652/1‑140 (MQ=255)                                      aTCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACt                                                                                        <  1:585833/140‑1 (MQ=255)                                        cTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGAATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCa                                                                                      <  1:83914/140‑1 (MQ=255)                                          ttttGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCacg                                                                                    >  1:715382/1‑140 (MQ=255)                                            ttGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCacgac                                                                                  >  2:692820/1‑140 (MQ=255)                                                  aTCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATc                                                                            >  2:1512569/1‑140 (MQ=255)                                                  aTCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATc                                                                            >  2:1170833/1‑140 (MQ=255)                                                          ggCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCCCTGTCGAGGATTACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCtt                                                                    <  2:1209379/140‑1 (MQ=255)                                                                     tCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCAcc                                                         >  1:557339/1‑140 (MQ=255)                                                                          aCGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGcc                                                    <  2:134256/140‑1 (MQ=255)                                                                          aCGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGcc                                                    <  1:476128/140‑1 (MQ=255)                                                                           cGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGccc                                                   <  1:1481578/140‑1 (MQ=255)                                                                             aGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTa                                                 <  2:1101444/140‑1 (MQ=255)                                                                                 tGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACg                                             >  1:1003537/1‑140 (MQ=255)                                                                                    tACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTaa                                          >  1:246489/1‑140 (MQ=255)                                                                                    tACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTaa                                          >  2:1106090/1‑140 (MQ=255)                                                                                     aCCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAg                                         >  1:1097031/1‑140 (MQ=255)                                                                                       cAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCa                                       <  1:692820/140‑1 (MQ=255)                                                                                        aGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCAc                                      >  2:1361437/1‑140 (MQ=255)                                                                                             aGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGcc                                 <  2:715382/140‑1 (MQ=255)                                                                                             aGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGcc                                 <  2:1496690/140‑1 (MQ=255)                                                                                             aGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGcc                                 <  1:1035652/140‑1 (MQ=255)                                                                                               gATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTa                                                 <  1:541088/122‑1 (MQ=255)                                                                                               gATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTa                                                 >  2:541088/1‑122 (MQ=255)                                                                                                aTTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTaa                              >  1:1239086/1‑140 (MQ=255)                                                                                                 ttAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAAc                             >  1:1307478/1‑140 (MQ=255)                                                                                                 ttAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAAc                             >  1:914329/1‑140 (MQ=255)                                                                                                  tAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGTGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACt                            >  1:195089/1‑140 (MQ=255)                                                                                                    aTTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCa                                       >  1:1512916/1‑127 (MQ=255)                                                                                                    aTTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCa                                       <  2:1512916/127‑1 (MQ=255)                                                                                                    aTTTCTCGACGGTTTGAATATCACTGTCGAGGAATACACCACCAAGTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCa                          >  2:1416676/1‑140 (MQ=255)                                                                                                         tcGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTc                     >  2:367754/1‑140 (MQ=255)                                                                                                         tcGACGGTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTc                     >  1:388057/1‑140 (MQ=255)                                                                                                              ggTTTGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTcgcgcg                <  2:1371137/140‑1 (MQ=255)                                                                                                                tttGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGcc              >  1:329280/1‑140 (MQ=255)                                                                                                                tttGAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGcc              >  2:623628/1‑140 (MQ=255)                                                                                                                   gAATATCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGt           <  1:1416676/140‑1 (MQ=255)                                                                                                                        tCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGc      <  1:1512569/140‑1 (MQ=255)                                                                                                                        tCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGc      <  1:367754/140‑1 (MQ=255)                                                                                                                        tCACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGc      <  1:1170833/140‑1 (MQ=255)                                                                                                                         cACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGcc     >  2:1402526/1‑140 (MQ=255)                                                                                                                         cACTGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGcc     >  1:883463/1‑140 (MQ=255)                                                                                                                            tGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTcgcgcg                <  2:1366005/126‑1 (MQ=255)                                                                                                                            tGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTcgcgcg                >  1:1366005/1‑126 (MQ=255)                                                                                                                            tGTCGAGGAATACACCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGCCAGc  >  1:63928/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                               GGCGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAAACGAGTTTGATACCAGTTGAGGATTAATTTCTCGACGGTTTGAATATCACTGTCGAGGAATACGCCACCAATTAATGCTTCGACGGTGTCGGCGAGAATTGACTCACGACGAAATCCACCGCTTTTAAGTTCACCTGGCCCTAAACGTAAGCACTCGCCTAACTCAAATTCGCGCGCCAGTTCCGCCAGC  >  NC_000913/2703562‑2703824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑