Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,853,222	T→C	W363R (TGG→CGG)	hypD →	Fe‑(CN)2CO cofactor assembly scaffold protein HypD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,853,222	0	T	C	100.0%	270.6 / NA	76	W363R (TGG→CGG)	hypD	Fe‑(CN)2CO cofactor assembly scaffold protein HypD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/41);  total (35/41)

GACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAG  >  NC_000913/2853087‑2853353                                                                                                                                        |                                                                                                                                    gACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTa                                                                                                                                 <  2:1428814/140‑1 (MQ=255) gACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTa                                                                                                                                 <  1:308706/140‑1 (MQ=255)  aCCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTAt                                                                                                                                >  1:25016/1‑140 (MQ=255)  aCCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTAt                                                                                                                                >  2:528000/1‑140 (MQ=255)       cgcgcgcgTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTa                                                                                                                           >  2:598493/1‑140 (MQ=255)         cgcgcgTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATc                                                                                                                         <  2:25016/140‑1 (MQ=255)         cgcgcgTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATc                                                                                                                         <  2:576763/140‑1 (MQ=255)              gTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTcag                                                                                                                    <  1:1338828/140‑1 (MQ=255)                       aGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGt                                                                                                           <  2:833781/140‑1 (MQ=255)                         gTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTGTCAGTATCGTCAGCAGGAGAGTGa                                                                                                         <  1:220824/140‑1 (MQ=255)                         gTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGa                                                                                                         >  1:209437/1‑140 (MQ=255)                         gTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGa                                                                                                         <  1:827014/140‑1 (MQ=255)                          tATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGaa                                                                                                        >  1:685276/1‑140 (MQ=255)                            ttAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGc                                                                                                      >  2:274781/1‑140 (MQ=255)                            ttAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGc                                                                                                      <  2:158758/140‑1 (MQ=255)                            ttAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGc                                                                                                      >  1:329063/1‑140 (MQ=255)                            ttAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGc                                                                                                      <  1:528000/140‑1 (MQ=255)                              aaCGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGa                                                                                                         <  1:239811/135‑1 (MQ=255)                              aaCGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGa                                                                                                         >  2:239811/1‑135 (MQ=255)                              aaCGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGt                                                                                                    <  2:209437/140‑1 (MQ=255)                               aCGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCTGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTg                                                                                                   <  2:395047/140‑1 (MQ=255)                                   gCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGaata                                                                                               >  2:802252/1‑140 (MQ=255)                                            aGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAAc                                                                                      <  1:386113/140‑1 (MQ=255)                                            aGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAAc                                                                                      >  1:856486/1‑140 (MQ=255)                                            aGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATACTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAAc                                                                                      <  1:841116/140‑1 (MQ=255)                                             gCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACt                                                                                     >  1:1449233/1‑140 (MQ=255)                                             gCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACt                                                                                     >  1:820075/1‑140 (MQ=255)                                               cGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCg                                                                                   >  1:301072/1‑140 (MQ=255)                                                 cATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGcc                                                                                 >  2:1052524/1‑140 (MQ=255)                                                  aTCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGccc                                                                                >  2:407309/1‑140 (MQ=255)                                                  aTCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGccc                                                                                >  2:1274631/1‑140 (MQ=255)                                                  aTCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGcc                                                                                 >  2:96244/1‑139 (MQ=255)                                                  aTCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGcc                                                                                 <  1:96244/139‑1 (MQ=255)                                                   tCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCa                                                                               >  1:94027/1‑140 (MQ=255)                                                       tGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGt                                                                           >  2:874911/1‑140 (MQ=255)                                                           cGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCATCTCGCCCACGGTAgcg                                                                       <  1:600001/140‑1 (MQ=255)                                                                    gTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAgg                                                              <  1:802252/140‑1 (MQ=255)                                                                    gTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAgg                                                              <  2:301072/140‑1 (MQ=255)                                                                    gTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAgg                                                              <  1:407309/140‑1 (MQ=255)                                                                     tAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGc                                                             <  2:1191263/140‑1 (MQ=255)                                                                       acacCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGa                                                           >  2:591129/1‑140 (MQ=255)                                                                          ccTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgc                                                        >  2:635440/1‑140 (MQ=255)                                                                          ccTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgc                                                        >  2:1136408/1‑140 (MQ=255)                                                                          ccTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgc                                                        <  1:874911/140‑1 (MQ=255)                                                                             gTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgcagc                                                     <  1:598493/140‑1 (MQ=255)                                                                                 tCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAAtt                                                 <  2:780008/140‑1 (MQ=255)                                                                                 tCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAAtt                                                 <  2:945981/140‑1 (MQ=255)                                                                                  ccTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTa                                                >  2:1066715/1‑140 (MQ=255)                                                                                   cTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTaa                                               >  2:774144/1‑140 (MQ=255)                                                                                   cTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTaa                                               >  2:691659/1‑140 (MQ=255)                                                                                     cAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgcagc                                                     <  2:132192/132‑1 (MQ=255)                                                                                     cAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATgcagc                                                     >  1:132192/1‑132 (MQ=255)                                                                                     cAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATc                                             >  2:206629/1‑140 (MQ=255)                                                                                      aaaCCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCa                                            <  2:329063/140‑1 (MQ=255)                                                                                        aCCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAGTAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAAtt                                                 >  2:755529/1‑133 (MQ=255)                                                                                        aCCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAGTAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAAtt                                                 <  1:755529/133‑1 (MQ=255)                                                                                          cgcgTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAg                                        >  2:724049/1‑140 (MQ=255)                                                                                           gcgTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGc                                       <  2:820075/140‑1 (MQ=255)                                                                                           gcgTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGc                                       <  2:685276/140‑1 (MQ=255)                                                                                           gcgTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGc                                       <  2:1357985/140‑1 (MQ=255)                                                                                           gcgTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGc                                       <  1:1052524/140‑1 (MQ=255)                                                                                              tttGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGTAGCAATTAATCAACAGCCTg                                    >  2:1254947/1‑140 (MQ=255)                                                                                                     cgcTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATgg                             <  1:635440/140‑1 (MQ=255)                                                                                                       cTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGaa                           >  2:794486/1‑140 (MQ=255)                                                                                                         gATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGc                         <  1:283291/140‑1 (MQ=255)                                                                                                         gATGGTTTCCTCCGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGc                         <  1:206629/140‑1 (MQ=255)                                                                                                                tcctccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGcc                  <  2:1409255/140‑1 (MQ=255)                                                                                                                 cctccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCa                 >  1:808248/1‑140 (MQ=255)                                                                                                                  ctccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCaa                >  1:358512/1‑140 (MQ=255)                                                                                                                  ctccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCaa                >  1:1337693/1‑140 (MQ=255)                                                                                                                   tccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAAc               <  1:274781/140‑1 (MQ=255)                                                                                                                   tccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAAc               <  2:803249/140‑1 (MQ=255)                                                                                                                    ccGAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAAcc              >  2:1377501/1‑140 (MQ=255)                                                                                                                      gAAGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCg            <  2:358512/140‑1 (MQ=255)                                                                                                                        aGGAGCGTGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGtg          <  1:605367/140‑1 (MQ=255)                                                                                                                               tGCGCCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAGCTCGCCCACGGTAACGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCa   <  1:1267302/140‑1 (MQ=255)                                                                                                                                gcgcCGCGCGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCag  <  1:724049/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GACCCGCGCGCGCGTTGTGGTGAGGTATTAACGGGCAAATGTAAGCCGCATCAATGCCCGCTGTTTGGTAACACCTGTAATCCTCAAACCGCGTTTGGTGCGCTGATGGTTTCCTCCGAAGGAGCGTGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAG  >  NC_000913/2853087‑2853353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑