Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,854,143	G→A	G297E (GGG→GAG)	hypE →	hydrogenase maturation protein, carbamoyl dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,854,143	0	G	A	100.0%	238.9 / NA	67	G297E (GGG→GAG)	hypE	hydrogenase maturation protein, carbamoyl dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/39);  total (28/39)

TAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCT  >  NC_000913/2854006‑2854279                                                                                                                                          |                                                                                                                                         tAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGgaga                                                                                                                                        >  2:37545/1‑140 (MQ=255)       tGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGAcg                                                                                                                                  <  2:1144427/140‑1 (MQ=255)        gCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGAcgc                                                                                                                                 >  1:134134/1‑140 (MQ=255)         ccGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGAcgcg                                                                                                                                >  2:850168/1‑140 (MQ=255)         ccGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGAcgcg                                                                                                                                >  2:386244/1‑140 (MQ=255)            tgcgtgGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACgcggcg                                                                                                                             <  1:268695/140‑1 (MQ=255)                 ggCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGAt                                                                                                                        <  2:134134/140‑1 (MQ=255)                       tGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGa                                                                                                                  >  2:418009/1‑140 (MQ=255)                            aTTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtgg                                                                                                             <  1:286398/140‑1 (MQ=255)                             ttGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggt                                                                                                            >  1:264775/1‑140 (MQ=255)                              tGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           <  2:643860/140‑1 (MQ=255)                              tGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           <  1:418009/140‑1 (MQ=255)                              tGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           <  2:1107829/140‑1 (MQ=255)                              tGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           <  2:264775/140‑1 (MQ=255)                                      cTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTaa                                                                                                   <  1:850168/140‑1 (MQ=255)                                        ggACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAg                                                                                                 >  1:559360/1‑140 (MQ=255)                                        ggACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAg                                                                                                 >  2:1124344/1‑140 (MQ=255)                                           cGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGgtg                                                                                              >  2:110136/1‑140 (MQ=255)                                              ccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           >  2:1388286/1‑124 (MQ=255)                                              ccTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAgtggtg                                                                                                           <  1:1388286/124‑1 (MQ=255)                                               cTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCg                                                                                          <  2:85101/140‑1 (MQ=255)                                                    cTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTg                                                                                     <  1:1124344/140‑1 (MQ=255)                                                    cTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTg                                                                                     <  1:203262/140‑1 (MQ=255)                                                    cTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTg                                                                                     <  1:1416834/140‑1 (MQ=255)                                                       tGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCg                                                                                  >  2:297216/1‑140 (MQ=255)                                                         ccAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGt                                                                                <  2:1458028/140‑1 (MQ=255)                                                             cGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGt                                                                            <  1:1453183/140‑1 (MQ=255)                                                                         aGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAAc                                                                >  1:1230755/1‑140 (MQ=255)                                                                              tAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAAcc                                                           <  1:1024787/140‑1 (MQ=255)                                                                                gCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCt                                                         <  1:935666/140‑1 (MQ=255)                                                                                gCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCt                                                         >  1:583539/1‑140 (MQ=255)                                                                                gCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCt                                                         >  1:1309689/1‑140 (MQ=255)                                                                                 cTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTc                                                        >  2:42443/1‑140 (MQ=255)                                                                                  tGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCg                                                       >  1:816249/1‑140 (MQ=255)                                                                                  tGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCg                                                       <  2:816249/140‑1 (MQ=255)                                                                                    ttGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGAt                                                     >  2:1087415/1‑140 (MQ=255)                                                                                     tGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACAATCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGAtt                                                    >  2:657721/1‑140 (MQ=255)                                                                                          gcaacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACca                                               <  1:37545/140‑1 (MQ=255)                                                                                            aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAAccc                                                          >  1:146640/1‑127 (MQ=255)                                                                                            aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAAccc                                                          <  2:146640/127‑1 (MQ=255)                                                                                            aacgcGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACcaca                                             >  2:571901/1‑140 (MQ=255)                                                                                              cgcgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACg                                           >  2:376212/1‑140 (MQ=255)                                                                                               gcgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGc                                          <  1:571901/140‑1 (MQ=255)                                                                                               gcgGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGc                                          <  1:297216/140‑1 (MQ=255)                                                                                                      agCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCg                                   >  1:601004/1‑140 (MQ=255)                                                                                                      agCAAGTGCTGGCAGCGTTACAATCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCg                                   <  1:657721/140‑1 (MQ=255)                                                                                                        cAAGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCt                                 <  2:1105632/140‑1 (MQ=255)                                                                                                         aaGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCtt                                <  2:553313/140‑1 (MQ=255)                                                                                                          aGTGCTGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTc                               <  1:661570/140‑1 (MQ=255)                                                                                                               tGGCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGt                          <  2:1230755/140‑1 (MQ=255)                                                                                                                ggCAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGta                         <  2:601004/140‑1 (MQ=255)                                                                                                                  cAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGaa                                      <  1:739732/125‑1 (MQ=255)                                                                                                                  cAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGaa                                      >  2:739732/1‑125 (MQ=255)                                                                                                                  cAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGtat                        <  2:1130866/139‑1 (MQ=255)                                                                                                                  cAGCGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGtat                        >  1:1130866/1‑139 (MQ=255)                                                                                                                     cGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGaa                                      <  2:338626/122‑1 (MQ=255)                                                                                                                     cGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGaa                                      >  1:338626/1‑122 (MQ=255)                                                                                                                     cGTTACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGc                    >  2:1475270/1‑140 (MQ=255)                                                                                                                       ttACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCta                  >  1:1117821/1‑140 (MQ=255)                                                                                                                       ttACATTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCta                  >  2:474044/1‑140 (MQ=255)                                                                                                                           aTTCCCATCCACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCtaataa              <  1:1475270/140‑1 (MQ=255)                                                                                                                                 aTCCACTGGAGAAAGACGCGGCGCTGATTAGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCt        >  2:1506233/1‑140 (MQ=255)                                                                                                                                   ccACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTaa      <  2:443902/140‑1 (MQ=255)                                                                                                                                   ccACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTaa      <  1:474044/140‑1 (MQ=255)                                                                                                                                   ccACTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTaa      <  1:42443/140‑1 (MQ=255)                                                                                                                                     aCTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAAt    <  1:386244/140‑1 (MQ=255)                                                                                                                                     aCTGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAAt    <  1:376212/140‑1 (MQ=255)                                                                                                                                       tGGAGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAAtct  <  2:1052270/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TAAACCTGCCGTGCGTGGCGTTTGCGAATTGCTGGGACTGGACGCCCTGAACTTTGCCAACGAAGGCAAACTAGTAATAGCTGTTGAACGCAACGCGGCAGAGCAAGTGCTGGCAGCGTTACATTCCCATCCACTGGGGAAAGACGCGGCGCTGATTGGTGAAGTGGTGGAACGTAAAGGTGTTCGTCTTGCCGGTCTGTATGGCGTGAAACGAACCCTCGATTTACCACACGCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCT  >  NC_000913/2854006‑2854279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑