Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	351,991	C→T	D259D (GAC→GAT)	prpD →	2‑methylcitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	351,991	0	C	T	100.0%	273.9 / NA	77	D259D (GAC→GAT)	prpD	2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/42);  total (35/42)

GCGCCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGACGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGCTCTATG  >  NC_000913/351854‑352127                                                                                                                                          |                                                                                                                                         gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGTCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  1:1463002/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTAGGATGt                                                                                                                                        >  2:1073455/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  2:870768/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  2:86830/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  2:538424/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  2:338201/1‑140 (MQ=255) gcgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGt                                                                                                                                        >  1:887712/1‑140 (MQ=255)  cgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtc                                                                                                                                       <  1:787344/140‑1 (MQ=255)  cgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtc                                                                                                                                       <  1:389675/140‑1 (MQ=255)  cgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtc                                                                                                                                       <  1:508440/140‑1 (MQ=255)  cgcCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtc                                                                                                                                       <  2:20351/140‑1 (MQ=255)   gcCGAACACCGGCACGCGTAAATCCTGAGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtct                                                                                                                                      <  2:1468632/140‑1 (MQ=255)    ccGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGtctc                                                                                                                                     >  2:516020/1‑140 (MQ=255)      gAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCt                                                                                                                                   >  2:321239/1‑140 (MQ=255)      gAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCt                                                                                                                                   <  2:664231/140‑1 (MQ=255)            cGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAg                                                                                                                             >  1:484132/1‑140 (MQ=255)                 cgcgTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGaa                                                                                                                        >  1:996234/1‑140 (MQ=255)                 cgcgTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGaa                                                                                                                        >  2:1033524/1‑140 (MQ=255)                 cgcgTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGaa                                                                                                                        >  2:96846/1‑140 (MQ=255)                 cgcgTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGaa                                                                                                                        <  2:1097536/140‑1 (MQ=255)                         tCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGTCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCg                                                                                                                <  2:1463002/140‑1 (MQ=255)                            tGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCtt                                                                                                             <  1:162272/140‑1 (MQ=255)                            tGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTCATGGCGAAAACGGGCGAAATGGGTTACCCGGCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCtt                                                                                                             <  1:269897/140‑1 (MQ=255)                              ggcggcGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTcc                                                                                                           >  2:1488808/1‑140 (MQ=255)                              ggcggcGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTcc                                                                                                           <  2:996234/140‑1 (MQ=255)                              ggcggcGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGGCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTcc                                                                                                           <  2:887712/140‑1 (MQ=255)                                    gggcgATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGcc                                                                                                     >  2:273218/1‑140 (MQ=255)                                       cgATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGt                                                                                                  <  1:409551/140‑1 (MQ=255)                                        gATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTa                                                                                                 <  2:1450941/140‑1 (MQ=255)                                        gATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTa                                                                                                 <  1:321239/140‑1 (MQ=255)                                        gATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTa                                                                                                 <  2:484132/140‑1 (MQ=255)                                        gATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTa                                                                                                 <  2:352109/140‑1 (MQ=255)                                        gATGCCACTTCCCGCGCGGTACGTCTGGCACTGATCGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTa                                                                                                 <  2:382247/140‑1 (MQ=255)                                                ttCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAg                                                                                                         <  2:1252023/124‑1 (MQ=255)                                                ttCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAg                                                                                                         >  1:1252023/1‑124 (MQ=255)                                                ttCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCt                                                                                         <  1:96846/140‑1 (MQ=255)                                                ttCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCt                                                                                         >  2:491567/1‑140 (MQ=255)                                                  cccGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTAc                                                                                       >  1:1037284/1‑140 (MQ=255)                                                    cgcgcgGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGt                                                                                     <  1:86830/140‑1 (MQ=255)                                                       gcgGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTAt                                                                                  <  1:338201/140‑1 (MQ=255)                                                                 tGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAAtgtg                                                                        <  1:273218/140‑1 (MQ=255)                                                                   gCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCt                                                                      >  2:569833/1‑140 (MQ=255)                                                                   gCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCt                                                                      >  2:889822/1‑140 (MQ=255)                                                                     aCTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGt                                                                    >  1:634454/1‑140 (MQ=255)                                                                     aCTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGt                                                                    >  1:1171036/1‑140 (MQ=255)                                                                     aCTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGt                                                                    >  1:234902/1‑140 (MQ=255)                                                                         aTGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCaa                                                                >  2:926153/1‑140 (MQ=255)                                                                                         aaaTGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCcggcg                                                <  2:1037284/140‑1 (MQ=255)                                                                                         aaaTGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCcggcg                                                <  1:1472441/140‑1 (MQ=255)                                                                                           aTGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGa                                              <  1:926153/140‑1 (MQ=255)                                                                                            tGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAg                                             >  2:533660/1‑140 (MQ=255)                                                                                                    ccGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTcc                                                     >  2:1486317/1‑124 (MQ=255)                                                                                                    ccGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTcc                                                     <  1:1486317/124‑1 (MQ=255)                                                                                                    ccGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTcc                                                     <  1:762117/124‑1 (MQ=255)                                                                                                    ccGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTcc                                                     >  2:762117/1‑124 (MQ=255)                                                                                                       tCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCa                                  >  2:1258064/1‑140 (MQ=255)                                                                                                        cAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAg                                 >  2:657923/1‑140 (MQ=255)                                                                                                         aGCCCTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGa                                <  1:1258064/140‑1 (MQ=255)                                                                                                          gCCCTGACTGCGCCGGTGTGGGACTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGAc                               <  1:1073455/140‑1 (MQ=255)                                                                                                           cccTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACg                              <  2:234902/140‑1 (MQ=255)                                                                                                           cccTGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACg                              >  2:171595/1‑140 (MQ=255)                                                                                                              tGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCa                           <  1:491567/140‑1 (MQ=255)                                                                                                              tGACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCa                           >  2:161736/1‑140 (MQ=255)                                                                                                               gACTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCTCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAg                          <  2:1391525/140‑1 (MQ=255)                                                                                                                aCTGCGCCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGt                         >  2:625458/1‑140 (MQ=255)                                                                                                                   gcgcCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGa                      <  1:625458/140‑1 (MQ=255)                                                                                                                    cgcCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGaa                     >  2:1215456/1‑140 (MQ=255)                                                                                                                    cgcCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGaa                     >  2:1132938/1‑140 (MQ=255)                                                                                                                     gcCGGTGTGGGGCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAag                    >  1:566087/1‑140 (MQ=255)                                                                                                                               ggCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGAc          <  2:566087/140‑1 (MQ=255)                                                                                                                               ggCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGAc          <  1:657923/140‑1 (MQ=255)                                                                                                                               ggCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGAc          <  1:1033524/140‑1 (MQ=255)                                                                                                                                gCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACg         <  1:1488808/140‑1 (MQ=255)                                                                                                                                gCTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACg         <  2:1226720/140‑1 (MQ=255)                                                                                                                                 cTTCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGc        <  1:516020/140‑1 (MQ=255)                                                                                                                                  ttCTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGct       <  1:1132938/140‑1 (MQ=255)                                                                                                                                    cTACGATGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGctct     <  1:538424/140‑1 (MQ=255)                                                                                                                                       cgatgTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGCTCTATg  <  1:1215456/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCGCCGAACACCGGCACGCGTAAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAAACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGACGTCTCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAATGTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCGATGACGCTCTATG  >  NC_000913/351854‑352127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑