Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,091,562	T→C	G143G (GGT→GGC)	rsmE →	16S rRNA m(3)U1498 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,091,562	0	T	C	100.0%	285.1 / NA	80	G143G (GGT→GGC)	rsmE	16S rRNA m(3)U1498 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/46);  total (34/46)

AATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGC  >  NC_000913/3091426‑3091697                                                                                                                                         |                                                                                                                                        aaTCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGt                                                                                                                                      >  2:531184/1‑140 (MQ=255)        cgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCggg                                                                                                                               <  1:531184/140‑1 (MQ=255)        cgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCggg                                                                                                                               <  1:760301/140‑1 (MQ=255)        cgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCggg                                                                                                                               <  2:1394904/140‑1 (MQ=255)        cgaACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCggg                                                                                                                               <  1:786572/140‑1 (MQ=255)          aaCTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTg                                                                                                                             >  2:1209032/1‑140 (MQ=255)                 tgtAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGaaa                                                                                                                      >  2:782128/1‑140 (MQ=255)                     aGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCg                                                                                                                  >  2:103369/1‑140 (MQ=255)                     aGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCg                                                                                                                  >  2:232880/1‑140 (MQ=255)                       ccTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTc                                                                                                                <  1:779923/140‑1 (MQ=255)                        cTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCGATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTcc                                                                                                               <  2:544234/140‑1 (MQ=255)                         tCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCa                                                                                                              >  1:1190581/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:1305383/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:332681/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:1363594/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:137830/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  2:213661/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:282134/1‑140 (MQ=255)                           aTTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGc                                                                                                            >  1:802846/1‑140 (MQ=255)                            ttACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCg                                                                                                           <  1:103369/140‑1 (MQ=255)                            ttACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCg                                                                                                           <  2:148970/140‑1 (MQ=255)                            ttACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCg                                                                                                           <  2:282134/140‑1 (MQ=255)                             tACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGa                                                                                                          <  1:365983/140‑1 (MQ=255)                             tACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGa                                                                                                          <  1:107923/140‑1 (MQ=255)                               cGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATg                                                                                                        <  1:213661/140‑1 (MQ=255)                               cGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATg                                                                                                        >  1:437083/1‑140 (MQ=255)                                gCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATgg                                                                                                       <  2:1190581/140‑1 (MQ=255)                                 ccACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGAt                                                                                                         <  2:1229652/137‑1 (MQ=255)                                 ccACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGAt                                                                                                         >  1:1229652/1‑137 (MQ=255)                                 ccACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGa                                                                                                      >  2:146004/1‑140 (MQ=255)                                     ttttttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTg                                                                                                  <  2:91780/140‑1 (MQ=255)                                      tttttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTgg                                                                                                 <  1:1397753/140‑1 (MQ=255)                                       ttttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGa                                                                                                >  1:1036051/1‑140 (MQ=255)                                        tttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGaa                                                                                               <  1:1490882/140‑1 (MQ=255)                                        tttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGaa                                                                                               <  2:1195376/140‑1 (MQ=255)                                        tttCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGaa                                                                                               <  1:1360760/140‑1 (MQ=255)                                          tCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGc                                                                                             <  2:390281/140‑1 (MQ=255)                                             gAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTg                                                                                          <  2:1363594/140‑1 (MQ=255)                                             gAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTg                                                                                          <  2:1305383/140‑1 (MQ=255)                                             gAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTg                                                                                          <  2:802846/140‑1 (MQ=255)                                             gAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTg                                                                                          <  1:782128/140‑1 (MQ=255)                                             gAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTg                                                                                          <  1:232880/140‑1 (MQ=255)                                              aGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTgg                                                                                         >  1:1480257/1‑140 (MQ=255)                                               gcgcTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGgt                                                                                        <  1:146004/140‑1 (MQ=255)                                                  cTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGtgtg                                                                                     <  2:1036051/140‑1 (MQ=255)                                                  cTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGtgtg                                                                                     <  1:877516/140‑1 (MQ=255)                                                  cTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGtgtg                                                                                     <  1:39258/140‑1 (MQ=255)                                                  cTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGtgtg                                                                                     <  2:873529/140‑1 (MQ=255)                                                     cggcgTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCag                                                                                  >  2:1365896/1‑140 (MQ=255)                                                       gcgTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCagag                                                                                <  2:1242537/140‑1 (MQ=255)                                                             aaCTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGgtgt                                                                                      <  1:833699/128‑1 (MQ=255)                                                             aaCTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGgtgt                                                                                      >  2:833699/1‑128 (MQ=255)                                                                    tAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGac                                                                   <  1:1065247/140‑1 (MQ=255)                                                                            gTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaactg                                                           >  2:1074579/1‑140 (MQ=255)                                                                            gTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaactg                                                           >  1:723539/1‑140 (MQ=255)                                                                                 aaCAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaact                                                            <  2:148022/134‑1 (MQ=255)                                                                                 aaCAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaact                                                            >  1:148022/1‑134 (MQ=255)                                                                                 aaCAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCt                                                      >  1:1194315/1‑140 (MQ=255)                                                                                      gaagCTTGAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCAcc                                                 >  1:154677/1‑140 (MQ=255)                                                                                        agCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaact                                                            <  1:66278/127‑1 (MQ=255)                                                                                        agCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGactgaaact                                                            >  2:66278/1‑127 (MQ=255)                                                                                         gCTTGAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCcgc                                              <  2:154677/140‑1 (MQ=255)                                                                                         gCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCcgc                                              <  1:739142/140‑1 (MQ=255)                                                                                         gCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCcgc                                              <  1:515422/140‑1 (MQ=255)                                                                                           ttCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACAcgcgc                                            >  2:820747/1‑140 (MQ=255)                                                                                              agcagTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGcc                                         >  1:313836/1‑140 (MQ=255)                                                                                                 agTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGt                                      <  2:313836/140‑1 (MQ=255)                                                                                                   tGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTaa                                    >  1:152969/1‑140 (MQ=255)                                                                                                   tGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTaa                                    >  1:757947/1‑140 (MQ=255)                                                                                                   tGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTaa                                    <  2:462389/140‑1 (MQ=255)                                                                                                   tGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTaa                                    >  1:1398569/1‑140 (MQ=255)                                                                                                   tGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTaa                                    <  2:757947/140‑1 (MQ=255)                                                                                                     gCAGAAGATTGCAATTGCTGCCTGTGAGTAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACa                                  <  2:183259/140‑1 (MQ=255)                                                                                                              tGCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATa                         >  1:549229/1‑140 (MQ=255)                                                                                                               gCAATTGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATAc                        <  2:1398569/140‑1 (MQ=255)                                                                                                                   ttGCTGCCTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTg                    <  1:1074579/140‑1 (MQ=255)                                                                                                                         ccTGTGAGCAGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTa              <  1:1259265/140‑1 (MQ=255)                                                                                                                                  aGTGTGGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGaa     <  2:549229/140‑1 (MQ=255)                                                                                                                                    tgtgGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAAcg   >  2:856535/1‑140 (MQ=255)                                                                                                                                     gtgGCCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAAcgc  >  2:1298209/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AATCGATCGAACTCGGTGTAAGCCTCATTACGCCACTTTTTTCTGAGCGCTGCGGCGTTAAACTGGATAGTGAACGTCTGAACAAGAAGCTTCAGCAGTGGCAGAAGATTGCAATTGCTGCCTGTGAGCAGTGTGGTCGTAACCGGGTGCCGGAAATCCGTCCAGCGATGGATCTGGAAGCCTGGTGTGCAGAGCAGGATGAAGGACTGAAACTGAATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGC  >  NC_000913/3091426‑3091697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑