Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,196,669	T→C	T29A (ACC→GCC)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,196,669	0	T	C	100.0%	269.7 / NA	75	T29A (ACC→GCC)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

CCGTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGTGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATACCATACT  >  NC_000913/3196534‑3196800                                                                                                                                        |                                                                                                                                      ccGTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTCAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCgggg                                                                                                                                   <  2:1352025/140‑1 (MQ=255)  cGTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  <  1:80887/140‑1 (MQ=255)  cGTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  <  2:1044703/140‑1 (MQ=255)   gTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGcc                                                                                                                                 <  2:478437/140‑1 (MQ=255)    tCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  <  1:584776/138‑1 (MQ=255)    tCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  >  2:584776/1‑138 (MQ=255)        cccGACCAGGCGTGCATTAGCACCGAGAGATGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTAcc                                                                                                                            <  2:929363/140‑1 (MQ=255)           gACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  <  1:496592/131‑1 (MQ=255)           gACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGc                                                                                                                                  >  2:496592/1‑131 (MQ=255)           gACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTGcccgg                                                                                                                         >  2:386048/1‑137 (MQ=255)           gACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGt                                                                                                                         >  2:571643/1‑140 (MQ=255)            aCCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAgga                                                                                                                        >  2:1052555/1‑138 (MQ=255)                  cGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATc                                                                                                                  <  1:386048/140‑1 (MQ=255)                  cGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATc                                                                                                                  >  1:788009/1‑140 (MQ=255)                  cGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATc                                                                                                                  >  1:756541/1‑140 (MQ=255)                  cGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATc                                                                                                                  >  1:67567/1‑140 (MQ=255)                     gCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑Ag                                                                                                              >  2:409335/1‑140 (MQ=255)                      cATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGc                                                                                                             >  1:5871/1‑140 (MQ=255)                      cATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGc                                                                                                             <  2:621465/140‑1 (MQ=255)                        ttAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcat                                                                                                           <  1:669895/140‑1 (MQ=255)                        ttAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcat                                                                                                           <  1:1280739/140‑1 (MQ=255)                        ttAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcat                                                                                                           <  1:571643/140‑1 (MQ=255)                          aGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatca                                                                                                         <  1:1052555/140‑1 (MQ=255)                           gCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatcac                                                                                                        <  1:457216/140‑1 (MQ=255)                           gCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatcac                                                                                                        <  1:247779/140‑1 (MQ=255)                           gCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatcac                                                                                                        >  1:79316/1‑140 (MQ=255)                           gCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatcac                                                                                                        >  1:42048/1‑140 (MQ=255)                           gCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGGACCCGTAACGAGCC‑AGcatcac                                                                                                        >  2:247779/1‑140 (MQ=255)                            cACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGcatcaca                                                                                                       <  2:42048/140‑1 (MQ=255)                                gagagaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCatcacatcac                                                                                                   >  1:983359/1‑140 (MQ=255)                                gagagaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCatcacatcac                                                                                                   >  1:171298/1‑140 (MQ=255)                                gagagaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCatcacatcac                                                                                                   >  1:1246862/1‑140 (MQ=255)                                 agagaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCCTACCAGTAACGATTC‑AGCATCACATCacc                                                                                                  >  2:509401/1‑140 (MQ=255)                                   agaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCaccaa                                                                                                <  2:638443/140‑1 (MQ=255)                                   agaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCaccaa                                                                                                <  2:5871/140‑1 (MQ=255)                                    gaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCaccaac                                                                                               <  2:756541/140‑1 (MQ=255)                                    gaAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCaccaac                                                                                               <  2:788009/140‑1 (MQ=255)                                                  cGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTg                                                                                 >  1:705816/1‑140 (MQ=255)                                                  cGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTg                                                                                 <  2:569566/140‑1 (MQ=255)                                                    ccACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCa                                                                               <  1:444692/140‑1 (MQ=255)                                                     cACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCAc                                                                              >  2:1261083/1‑140 (MQ=255)                                                     cACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCAc                                                                              >  2:1350464/1‑140 (MQ=255)                                                        gTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGtt                                                                           <  1:1350464/140‑1 (MQ=255)                                                          tAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCa                                                                         <  1:1397496/140‑1 (MQ=255)                                                          tAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCa                                                                         <  1:1446316/140‑1 (MQ=255)                                                          tAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCa                                                                         <  2:1047168/140‑1 (MQ=255)                                                          tAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCACTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCa                                                                         <  1:821166/140‑1 (MQ=255)                                                            gccgcgccgcCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCaaa                                                                       >  2:28481/1‑140 (MQ=255)                                                                   cgccCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGc                                                                >  2:402019/1‑140 (MQ=255)                                                                                    aTGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTcc                                              <  2:79316/140‑1 (MQ=255)                                                                                        tATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGtc                                          >  2:1388102/1‑140 (MQ=255)                                                                                        tATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGtc                                          >  1:1270957/1‑140 (MQ=255)                                                                                               tttAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTgag                                   <  2:708351/140‑1 (MQ=255)                                                                                                  aaCCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCCAAGCA‑CCCCTCACCAACCACCAACCCTCCTTCACGTTCAAACTCTTGCCGCGGTACTTTC‑ATTCCCGTCTCCTgagaga                                >  2:269659/1‑140 (MQ=255)                                                                                                  aaCCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTgagaga                                >  1:1514455/1‑140 (MQ=255)                                                                                                  aaCCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTgagaga                                >  1:855729/1‑140 (MQ=255)                                                                                                  aaCCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAAAGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTgagaga                                >  2:14127/1‑140 (MQ=255)                                                                                                    ccACGGGCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGAtt                              >  2:1284553/1‑140 (MQ=255)                                                                                                         ggCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCaaaa                         >  2:740364/1‑140 (MQ=255)                                                                                                         ggCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCaaaa                         >  2:679018/1‑140 (MQ=255)                                                                                                         ggCACCGGCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCaaaa                         >  2:60180/1‑140 (MQ=255)                                                                                                             ccGGCGCTTCCGGCGAGATACGACTGGCGGAGCCGTAACAGTAACCAACC‑CACATCACAAAAAAAAAAACCATAACTCCCTCACCATCAAAATCTGGCAGAGATAATTTCTATCCCTTTC‑CCAGCGAGAATCAAAAtttt                     >  2:142259/1‑139 (MQ=255)                                                                                                               ggCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcg                   <  2:983359/140‑1 (MQ=255)                                                                                                               ggCGCTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcg                   <  2:67567/140‑1 (MQ=255)                                                                                                                 cgcTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcgcg                 <  1:28481/140‑1 (MQ=255)                                                                                                                 cgcTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcgcg                 <  2:1246862/140‑1 (MQ=255)                                                                                                                 cgcTTCCGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcgcg                 <  2:1514455/140‑1 (MQ=255)                                                                                                                       cGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGataa           <  1:402019/140‑1 (MQ=255)                                                                                                                       cGGCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGataa           <  1:409335/140‑1 (MQ=255)                                                                                                                        ggCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcgcg                 >  2:502461/1‑133 (MQ=255)                                                                                                                        ggCGAGATACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTgcgcg                 <  1:502461/133‑1 (MQ=255)                                                                                                                           gagaTACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATAcc       <  1:1261083/140‑1 (MQ=255)                                                                                                                           gagaTACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATAcc       <  1:679018/140‑1 (MQ=255)                                                                                                                           gagaTACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATAcc       <  1:1035559/140‑1 (MQ=255)                                                                                                                               tACGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATACCATAc   <  2:705816/140‑1 (MQ=255)                                                                                                                                aCGACTGGCGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATACCATACt  >  1:420405/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCGTCAACCCGACCAGGCGTGCATTAGCACCGAGAGAAGCGATATTCATCGCCACGTTAGCCGCGCCGCCCGGACGTTCTTCGATGGTATTCACTTTAACCACGGGCACCGGCGCTTCCGGCGAGATACGACTGGTGGGGCCGTACCAGTAACGATCC‑AGCATCACATCACCAACCACCATCACTCCTGCACGTTCAAACTCTGGCAGCGTTACTTTC‑ATTCCTGTCTCCTGAGAGATTCAAAATTTGCGCGCGATAATACCATACT  >  NC_000913/3196534‑3196800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑