Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,290,285	A→G	D491G (GAC→GGC)	yraJ →	putative fimbrial usher protein YraJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,290,285	0	A	G	100.0%	336.0 / NA	95	D491G (GAC→GGC)	yraJ	putative fimbrial usher protein YraJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (57/38);  total (57/38)

TTTTCATGGCTATCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATATCTCTG  >  NC_000913/3290151‑3290416                                                                                                                                       |                                                                                                                                    ttttCATGGCTATCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCtata                                                                                                                                >  1:1466850/1‑140 (MQ=255)  tttCATGGCTATCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATaa                                                                                                                               <  1:1297073/140‑1 (MQ=255)  tttCATGGCTATCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATCGGGGCTATaa                                                                                                                               >  2:133360/1‑140 (MQ=255)             tCAATGGCGTACGCAGTACATCAATGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGTTGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaaa                                                                                                                    >  1:346731/1‑140 (MQ=255)             tCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaaa                                                                                                                    >  1:799377/1‑140 (MQ=255)             tCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaaa                                                                                                                    >  1:1296912/1‑140 (MQ=255)              cAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaaaa                                                                                                                   <  2:977876/140‑1 (MQ=255)                    cGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGa                                                                                                             >  2:1224297/1‑140 (MQ=255)                      tACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaa                                                                                                                     <  2:122461/130‑1 (MQ=255)                      tACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCaa                                                                                                                     >  1:122461/1‑130 (MQ=255)                           aGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaa                                                                                                      >  1:1372933/1‑140 (MQ=255)                             tACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaatt                                                                                                    >  2:1075624/1‑140 (MQ=255)                             tACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaatt                                                                                                    <  2:1372933/140‑1 (MQ=255)                             tACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaatt                                                                                                    >  2:855293/1‑140 (MQ=255)                             tACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaatt                                                                                                    >  1:1110917/1‑140 (MQ=255)                              aCATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAaattcaattc                                                                                                   >  1:652087/1‑140 (MQ=255)                                aTCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAAttcaattcaa                                                                                                 >  1:1079504/1‑140 (MQ=255)                                aTCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAAttcaattcaa                                                                                                 >  2:318617/1‑140 (MQ=255)                                 tCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAAc                                                                                                <  1:1481401/140‑1 (MQ=255)                                  cAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACa                                                                                               <  2:1296912/140‑1 (MQ=255)                                  cAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACa                                                                                               >  2:242388/1‑140 (MQ=255)                                     aGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCa                                                                                            >  2:330933/1‑140 (MQ=255)                                     aGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCa                                                                                            >  1:134436/1‑140 (MQ=255)                                       atatCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGc                                                                                          >  1:1068630/1‑140 (MQ=255)                                       atatCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGc                                                                                          >  2:1140279/1‑140 (MQ=255)                                       atatCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGc                                                                                          <  2:1068630/140‑1 (MQ=255)                                       atatCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGc                                                                                          >  2:796493/1‑140 (MQ=255)                                        tatCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGcc                                                                                         <  2:652087/140‑1 (MQ=255)                                         atCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCa                                                                                        <  2:1110917/140‑1 (MQ=255)                                           cccGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGa                                                                                      >  1:1182314/1‑140 (MQ=255)                                           cccGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGa                                                                                      >  1:468312/1‑140 (MQ=255)                                           cccGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGa                                                                                      >  1:1412573/1‑140 (MQ=255)                                           cccGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGa                                                                                      <  2:813469/140‑1 (MQ=255)                                           cccGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGa                                                                                      <  1:1211888/140‑1 (MQ=255)                                               gAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCa                                                                                        <  1:420576/134‑1 (MQ=255)                                               gAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCa                                                                                        >  2:420576/1‑134 (MQ=255)                                               gAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAat                                                                                  >  1:1086216/1‑140 (MQ=255)                                               gAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAat                                                                                  >  2:1500525/1‑140 (MQ=255)                                                          cAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATgggg                                                                       <  1:1075624/140‑1 (MQ=255)                                                          cAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATgggg                                                                       <  2:1101641/140‑1 (MQ=255)                                                          cAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATgggg                                                                       <  1:1500525/140‑1 (MQ=255)                                                            atatCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTa                                                                     >  1:743407/1‑140 (MQ=255)                                                            atatCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTa                                                                     >  1:1499282/1‑140 (MQ=255)                                                             tatCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATCGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTaa                                                                    <  1:133360/140‑1 (MQ=255)                                                              atCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAg                                                                   >  2:49344/1‑140 (MQ=255)                                                              atCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCG‑AATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGt                                                                  <  1:790003/140‑1 (MQ=255)                                                               tCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGt                                                                  <  1:1477033/140‑1 (MQ=255)                                                                 gCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCt                                                                >  2:1248862/1‑140 (MQ=255)                                                                  cTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTg                                                               <  2:743407/140‑1 (MQ=255)                                                                  cTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTg                                                               >  1:111658/1‑140 (MQ=255)                                                                    gTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTa                                                             >  2:1013365/1‑140 (MQ=255)                                                                    gTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTa                                                             >  1:668654/1‑140 (MQ=255)                                                                    gTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTa                                                             <  2:739225/140‑1 (MQ=255)                                                                      cAGCTACTTTCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATg                                                           >  1:241584/1‑140 (MQ=255)                                                                             taTCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCgg                                                    >  1:1517520/1‑140 (MQ=255)                                                                               tCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGtt                                                  <  2:668654/140‑1 (MQ=255)                                                                                     taCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagc                                            <  2:166785/140‑1 (MQ=255)                                                                                     taCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagc                                            <  2:1466850/140‑1 (MQ=255)                                                                                     taCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagc                                            >  2:811148/1‑140 (MQ=255)                                                                                     taCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagc                                            <  2:1086216/140‑1 (MQ=255)                                                                                     taCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagc                                            <  2:1295414/140‑1 (MQ=255)                                                                                      aCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagca                                           >  1:805772/1‑140 (MQ=255)                                                                                      aCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagca                                           >  1:951120/1‑140 (MQ=255)                                                                                      aCCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCgcagca                                           >  1:200296/1‑140 (MQ=255)                                                                                       ccAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCaa                                          <  2:1412573/140‑1 (MQ=255)                                                                                        cAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAg                                         <  2:1499282/140‑1 (MQ=255)                                                                                         aaCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGa                                        >  2:1102407/1‑140 (MQ=255)                                                                                          aCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCTGGTTCGCAGCAAGAc                                       >  2:615611/1‑140 (MQ=255)                                                                                          aCGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGAc                                       >  2:605065/1‑140 (MQ=255)                                                                                           cGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACt                                      >  2:1115572/1‑140 (MQ=255)                                                                                           cGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACt                                      >  2:1410566/1‑140 (MQ=255)                                                                                            gATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCC‑AAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTAt                                    <  2:191588/140‑1 (MQ=255)                                                                                               ggCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTg                                  >  2:698926/1‑140 (MQ=255)                                                                                               ggCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTg                                  >  2:1453664/1‑140 (MQ=255)                                                                                               ggCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTg                                  >  1:912736/1‑140 (MQ=255)                                                                                                 cTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTggg                                >  1:1086273/1‑140 (MQ=255)                                                                                                            ttAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGAt                     >  1:1154311/1‑140 (MQ=255)                                                                                                            ttAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGAt                     <  1:811148/140‑1 (MQ=255)                                                                                                            ttAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGAt                     <  2:1086273/140‑1 (MQ=255)                                                                                                             tAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATa                    <  2:346731/140‑1 (MQ=255)                                                                                                             tAATGAAGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATa                    <  2:614182/140‑1 (MQ=255)                                                                                                                   aGCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACa              >  2:392454/1‑140 (MQ=255)                                                                                                                    gCCAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAg             <  1:1155144/140‑1 (MQ=255)                                                                                                                     ccAATACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAgg            <  1:143820/140‑1 (MQ=255)                                                                                                                         tACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAata        >  2:1445189/1‑140 (MQ=255)                                                                                                                         tACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAata        >  2:1146729/1‑140 (MQ=255)                                                                                                                         tACCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAata        >  1:1306521/1‑140 (MQ=255)                                                                                                                          aCCCGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAatat       <  1:392454/140‑1 (MQ=255)                                                                                                                            ccGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATAtct     <  2:912736/140‑1 (MQ=255)                                                                                                                             cGCAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATAtctc    <  2:1182314/140‑1 (MQ=255)                                                                                                                              gCAATTGGGGCTATAACAGTCGCCAAAAAAGCGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCCCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATATctct   >  2:978364/1‑140 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAAc               <  1:526522/127‑1 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAAc               >  2:526522/1‑127 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATATCTCTg  >  1:1219082/1‑140 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATATCTCTg  >  1:914433/1‑140 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTAATGGGGCAATAACGATAAAAACAGGAATAtct     >  2:759642/1‑137 (MQ=255)                                                                                                                               cAATTGGGGCTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTAATGGGGCAATAACGATAAAAACAGGAATAtct     <  1:759642/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TTTTCATGGCTATCAATGGCGTACGCAGTACATCAAAGATATCCCGGAAACCAACACCAATATCGCTGTCAGCTACTATCGCTATACCAACGATGGCTATTTTAGTTTTAATGAAGCCAATACCCGCAATTGGGACTATAACAGTCGCCAAAAAAGTGAAATTCAATTCAACATCAGCCAGACAATATTTGATGGGGTAAGTCTGTATGCCTCCGGTTCGCAGCAAGACTATTGGGGCAATAACGATAAAAACAGGAATATCTCTG  >  NC_000913/3290151‑3290416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑