Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,829	G→A	intergenic (+28/+28)	yhcN → / ← yhcO	DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,829	0	G	A	100.0%	269.8 / NA	83	intergenic (+28/+28)	yhcN/yhcO	DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/34);  total (49/34)

TGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATG  >  NC_000913/3385696‑3385961                                                                                                                                      |                                                                                                                                     tGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCg                                                                                                                                >  1:1246031/1‑140 (MQ=255)  gAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCgg                                                                                                                               >  2:768528/1‑140 (MQ=255)      tGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCg                                                                                                                           <  1:588901/140‑1 (MQ=255)       gCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCgg                                                                                                                          >  2:943076/1‑140 (MQ=255)       gCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCgg                                                                                                                          <  2:1370691/140‑1 (MQ=255)         tGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCgggg                                                                                                                        <  1:561188/140‑1 (MQ=255)         tGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCgggg                                                                                                                        >  2:286429/1‑140 (MQ=255)          gAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCggggg                                                                                                                       >  2:447194/1‑140 (MQ=255)            aCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCt                                                                                                                     <  1:212383/140‑1 (MQ=255)                  aaGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCttttttt                                                                                                               >  2:625030/1‑140 (MQ=255)                      ggAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                           <  1:1241965/140‑1 (MQ=255)                      ggAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                           <  2:1513795/140‑1 (MQ=255)                               aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                  <  1:943076/140‑1 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                           >  2:313783/1‑128 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgcg                                                                                                           <  1:313783/128‑1 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  2:1014665/1‑140 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  2:773649/1‑140 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  1:1255124/1‑140 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  1:888564/1‑140 (MQ=255)                                  cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  1:866341/1‑140 (MQ=255)                                     aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                  <  1:1512439/134‑1 (MQ=255)                                     aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                  >  2:1512439/1‑134 (MQ=255)                                         gCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaaaa                                                                                        <  1:1248831/140‑1 (MQ=255)                                            tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGc                                                                                     >  2:1138488/1‑140 (MQ=255)                                               cAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCaaa                                                                                  <  2:866341/140‑1 (MQ=255)                                               cAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCaaa                                                                                  <  2:1246031/140‑1 (MQ=255)                                                    tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                             >  2:1499567/1‑140 (MQ=255)                                                         aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                        >  1:11870/1‑140 (MQ=255)                                                           gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         <  2:154003/137‑1 (MQ=255)                                                           gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         >  1:154003/1‑137 (MQ=255)                                                           gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                      <  1:447194/140‑1 (MQ=255)                                                           gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                      >  2:613700/1‑140 (MQ=255)                                                            cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     >  1:396929/1‑140 (MQ=255)                                                            cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     >  2:1106195/1‑140 (MQ=255)                                                            cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     >  1:453684/1‑140 (MQ=255)                                                            cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     <  1:1400683/140‑1 (MQ=255)                                                            cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     <  2:915846/140‑1 (MQ=255)                                                                tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                 >  2:312298/1‑140 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            <  1:1075062/140‑1 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            >  2:516409/1‑140 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            <  1:613700/140‑1 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            <  1:286429/140‑1 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            <  1:1130701/140‑1 (MQ=255)                                                                     gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                            >  2:1130701/1‑140 (MQ=255)                                                                        acacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         <  2:115313/124‑1 (MQ=255)                                                                        acacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         >  1:115313/1‑124 (MQ=255)                                                                             tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGC‑TTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                   >  1:322857/1‑140 (MQ=255)                                                                               gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                  >  2:1099810/1‑140 (MQ=255)                                                                                    cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                         >  2:533187/1‑128 (MQ=255)                                                                                    cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                         <  1:533187/128‑1 (MQ=255)                                                                                      aCGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAat                                           >  1:1179432/1‑140 (MQ=255)                                                                                       cGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                    <  2:184100/130‑1 (MQ=255)                                                                                       cGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                    >  1:184100/1‑130 (MQ=255)                                                                                        ggCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAatat                                         >  2:875488/1‑140 (MQ=255)                                                                                                 gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                >  1:1139526/1‑140 (MQ=255)                                                                                                  tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                               >  1:1049861/1‑124 (MQ=255)                                                                                                  tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                               <  2:1049861/124‑1 (MQ=255)                                                                                                  tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                               >  1:1017360/1‑140 (MQ=255)                                                                                                  tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                               >  2:584701/1‑140 (MQ=255)                                                                                                       aTAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACg                          >  1:1242875/1‑140 (MQ=255)                                                                                                         aaaCCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGc                        <  1:1499567/140‑1 (MQ=255)                                                                                                          aaCCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCg                       >  1:950959/1‑140 (MQ=255)                                                                                                           aCCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                      <  2:888564/140‑1 (MQ=255)                                                                                                           aCCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                      <  1:989327/140‑1 (MQ=255)                                                                                                            cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                      >  2:483593/1‑139 (MQ=255)                                                                                                            cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                      <  1:483593/139‑1 (MQ=255)                                                                                                            cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:383462/1‑140 (MQ=255)                                                                                                            cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCACCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  1:601730/1‑140 (MQ=255)                                                                                                                 aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  1:59730/1‑140 (MQ=255)                                                                                                                 aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  1:139767/1‑140 (MQ=255)                                                                                                                 aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  1:224030/1‑140 (MQ=255)                                                                                                                 aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  1:32466/1‑140 (MQ=255)                                                                                                                 aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  1:510522/1‑140 (MQ=255)                                                                                                                   cGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt              <  2:396929/140‑1 (MQ=255)                                                                                                                   cGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt              <  2:1242875/140‑1 (MQ=255)                                                                                                                   cGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACAGCGACGCGtttt              >  2:299626/1‑140 (MQ=255)                                                                                                                   cGTCTTGTCCGACGATATTACCCCCGGTCCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt              >  2:346358/1‑140 (MQ=255)                                                                                                                    gTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc             <  1:625030/140‑1 (MQ=255)                                                                                                                       ttGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct          <  1:1138488/140‑1 (MQ=255)                                                                                                                         gTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc        <  2:1139526/140‑1 (MQ=255)                                                                                                                         gTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc        <  1:312298/140‑1 (MQ=255)                                                                                                                          tCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc       >  1:755327/1‑140 (MQ=255)                                                                                                                               cgaTATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg  >  2:989180/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     TGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATG  >  NC_000913/3385696‑3385961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑