Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,559,459	T→C	L398L (TTG→CTG)	rtcR →	DNA‑binding transcriptional activator RtcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,559,459	0	T	C	100.0%	313.1 / NA	88	L398L (TTG→CTG)	rtcR	DNA‑binding transcriptional activator RtcR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/42);  total (46/42)

ATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGTTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGTCT  >  NC_000913/3559322‑3559595                                                                                                                                          |                                                                                                                                         aTCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAGGCGCGGCGCGCCTGGCTg                                                                                                                                        >  1:1330429/1‑140 (MQ=255)  tctcTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCctggctgg                                                                                                                                       <  2:478565/140‑1 (MQ=255)       ggACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCtttt                                                                                                                                  >  1:796309/1‑140 (MQ=255)            ttCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGAc                                                                                                                             <  1:149277/140‑1 (MQ=255)             tCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCctggctgg                                                                                                                                       <  2:1329676/129‑1 (MQ=255)             tCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCctggctgg                                                                                                                                       >  1:1329676/1‑129 (MQ=255)             tCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGAcc                                                                                                                            >  1:1501513/1‑140 (MQ=255)             tCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGAcc                                                                                                                            >  2:88656/1‑140 (MQ=255)             tCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGAcc                                                                                                                            <  1:1214511/140‑1 (MQ=255)              cACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAATTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAGGCGCGGCGCGCCTGGCTGGCTTTTGCGACct                                                                                                                           <  2:1330429/140‑1 (MQ=255)              cACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACct                                                                                                                           <  2:299729/140‑1 (MQ=255)                cccTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACctct                                                                                                                         >  2:1269107/1‑140 (MQ=255)                  cTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTcc                                                                                                                       >  2:638328/1‑140 (MQ=255)                   tGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTccc                                                                                                                      >  2:72207/1‑140 (MQ=255)                    gCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTcccc                                                                                                                     <  2:973731/140‑1 (MQ=255)                     ccGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCa                                                                                                                    <  1:487879/140‑1 (MQ=255)                                  agcgccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCACGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGgcgc                                                                                                       <  2:1086314/140‑1 (MQ=255)                                    cgccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCTTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCgg                                                                                                     >  2:1168787/1‑140 (MQ=255)                                    cgccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCgg                                                                                                     >  1:324810/1‑140 (MQ=255)                                    cgccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCgg                                                                                                     >  1:1070461/1‑140 (MQ=255)                                    cgccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCgg                                                                                                     >  1:1056637/1‑140 (MQ=255)                                       cagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTaa                                                                                                  <  2:498833/140‑1 (MQ=255)                                               tatTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTcgcg                                                                                          <  1:901434/140‑1 (MQ=255)                                               tatTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTcgcg                                                                                          <  2:143512/140‑1 (MQ=255)                                               tatTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTcgcg                                                                                          >  1:1489354/1‑140 (MQ=255)                                                    aaccgaaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACtt                                                                                     >  2:202228/1‑140 (MQ=255)                                                    aaccgaaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACtt                                                                                     >  1:7104/1‑140 (MQ=255)                                                    aaccgaaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACtt                                                                                     >  2:1268596/1‑140 (MQ=255)                                                      ccgaaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTc                                                                                   >  2:349398/1‑140 (MQ=255)                                                        gaaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTg                                                                                 >  2:920435/1‑140 (MQ=255)                                                         aaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGc                                                                                <  1:484221/140‑1 (MQ=255)                                                         aaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGc                                                                                <  2:1056637/140‑1 (MQ=255)                                                         aaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGc                                                                                >  1:696591/1‑140 (MQ=255)                                                         aaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGc                                                                                <  1:1391180/140‑1 (MQ=255)                                                         aaccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGc                                                                                >  2:181331/1‑140 (MQ=255)                                                           ccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCa                                                                              <  1:202228/140‑1 (MQ=255)                                                           ccTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCa                                                                              >  2:243781/1‑140 (MQ=255)                                                              ggATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCg                                                                           >  1:1273757/1‑140 (MQ=255)                                                                    tGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCAcgc                                                                     <  2:380626/140‑1 (MQ=255)                                                                    tGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCAcgc                                                                     >  1:930934/1‑140 (MQ=255)                                                                    tGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCAcgc                                                                     <  2:984755/140‑1 (MQ=255)                                                                         tGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATg                                                                >  1:171680/1‑140 (MQ=255)                                                                         tGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATg                                                                >  2:1438628/1‑140 (MQ=255)                                                                            aGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGcc                                                             >  1:803796/1‑140 (MQ=255)                                                                              cgcCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCAc                                                           >  1:966082/1‑140 (MQ=255)                                                                              cgcCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCAc                                                           >  1:296644/1‑140 (MQ=255)                                                                                ccACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCt                                                         <  2:796309/140‑1 (MQ=255)                                                                                 cACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCtt                                                        <  1:1446824/140‑1 (MQ=255)                                                                                   cGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCtccg                                                      >  2:562159/1‑137 (MQ=255)                                                                                   cGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTg                                                      >  2:1070982/1‑140 (MQ=255)                                                                                   cGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTg                                                      >  2:176762/1‑140 (MQ=255)                                                                                        cactcactGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGTGGATGGCCACCTTTGCCACt                                                 >  2:1133910/1‑140 (MQ=255)                                                                                           tcactGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGc                                              <  2:324810/140‑1 (MQ=255)                                                                                            cactGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCg                                             <  1:72207/140‑1 (MQ=255)                                                                                             actGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCgg                                            <  1:176762/140‑1 (MQ=255)                                                                                             actGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCgg                                            <  1:1070982/140‑1 (MQ=255)                                                                                             actGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCgg                                            <  1:88656/140‑1 (MQ=255)                                                                                               tGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGc                                              >  2:1292843/1‑136 (MQ=255)                                                                                               tGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGc                                              <  1:1292843/136‑1 (MQ=255)                                                                                               tGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGAc                                          >  2:472307/1‑140 (MQ=255)                                                                                               tGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGAc                                          >  2:834701/1‑140 (MQ=255)                                                                                                ggCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCgg                                            <  2:522442/137‑1 (MQ=255)                                                                                                ggCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCgg                                            >  1:522442/1‑137 (MQ=255)                                                                                                ggCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACg                                         >  2:578161/1‑140 (MQ=255)                                                                                                  cGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCa                                       <  2:1070461/140‑1 (MQ=255)                                                                                                  cGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCa                                       >  1:1128870/1‑140 (MQ=255)                                                                                                   gACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCAt                                      <  1:243781/140‑1 (MQ=255)                                                                                                   gACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCAt                                      <  1:578161/140‑1 (MQ=255)                                                                                                   gACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCAt                                      <  1:1268596/140‑1 (MQ=255)                                                                                                     cAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCa                                    >  1:71347/1‑140 (MQ=255)                                                                                                     cAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCa                                    >  1:442602/1‑140 (MQ=255)                                                                                                       gcgtgcgtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGTGGATGGCCACCTTTGCCACTAGCGGACGCATCAct                                  <  1:1133910/140‑1 (MQ=255)                                                                                                       gcgtgcgtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCAct                                  <  2:71347/140‑1 (MQ=255)                                                                                                        cgtgcgtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCActc                                 >  1:833723/1‑140 (MQ=255)                                                                                                           gcgtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTgg                              <  2:930934/140‑1 (MQ=255)                                                                                                             gtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGAc                            <  1:1269107/140‑1 (MQ=255)                                                                                                             gtTTTAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGACCACCTTTGCCACTAGCGGACGCATCACTCTGGAc                            <  1:293726/140‑1 (MQ=255)                                                                                                              ttttAACACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACg                           <  1:170051/140‑1 (MQ=255)                                                                                                                  aaCACCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGt                       >  2:1281869/1‑140 (MQ=255)                                                                                                                   acacCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGtt                      <  1:1281869/140‑1 (MQ=255)                                                                                                                    cacCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTg                     <  2:1128870/140‑1 (MQ=255)                                                                                                                     acCGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGa                    >  1:933704/1‑140 (MQ=255)                                                                                                                       cGAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAg                  <  1:181331/140‑1 (MQ=255)                                                                                                                        gAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGtt                      >  2:532049/1‑135 (MQ=255)                                                                                                                        gAAGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGtt                      <  1:532049/135‑1 (MQ=255)                                                                                                                         aaGCGCGGCGCGCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGAt                >  2:725532/1‑140 (MQ=255)                                                                                                                              cggcgcgCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGAt           >  2:473900/1‑140 (MQ=255)                                                                                                                              cggcgcgCCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGAt           >  2:189579/1‑140 (MQ=255)                                                                                                                                gcgcgcCTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATaa         <  2:7104/140‑1 (MQ=255)                                                                                                                                     cttggctggcTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGt    <  1:1168787/138‑1 (MQ=255)                                                                                                                                     ccTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGt    <  1:931642/140‑1 (MQ=255)                                                                                                                                     ccTGGCTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGt    <  1:472307/140‑1 (MQ=255)                                                                                                                                      ctggctggCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGTc   <  2:933704/140‑1 (MQ=255)                                                                                                                                       tggctggcTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGTCt  <  2:803796/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCGCGCCTGGTTGGCTTTTGCGACCTCTCCCCAGGCAACATGGCGCGGTAACTTTCGCGAACTTTCTGCCAGCGTCACGCGGATGGCCACCTTTGCCACTAGCGGACGCATCACTCTGGACGTGGTTGAAGATGAGATAAACCGTCT  >  NC_000913/3559322‑3559595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑