Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,954,748	T→C	V424A (GTG→GCG)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,954,748	0	T	C	100.0%	256.4 / NA	72	V424A (GTG→GCG)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/36);  total (36/36)

CCGCGCAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATTCTCG  >  NC_000913/3954611‑3954876                                                                                                                                          |                                                                                                                                 ccGCGCAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGgcgc                                                                                                                                >  2:249275/1‑140 (MQ=255)  cgcgCAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGct                                                                                                                               <  2:856669/140‑1 (MQ=255)   gcgcAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGctc                                                                                                                              <  1:1367728/140‑1 (MQ=255)     gcAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTa                                                                                                                            <  2:931862/140‑1 (MQ=255)        ggTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACgg                                                                                                                         <  1:215483/140‑1 (MQ=255)             tGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACt                                                                                                                    <  1:249275/140‑1 (MQ=255)               cAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACttt                                                                                                                  <  2:1407094/140‑1 (MQ=255)                   cATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGct                                                                                                                               <  1:498499/123‑1 (MQ=255)                   cATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGct                                                                                                                               >  2:498499/1‑123 (MQ=255)                    aTTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGa                                                                                                             >  1:60999/1‑140 (MQ=255)                     ttCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGaa                                                                                                            <  1:769780/140‑1 (MQ=255)                       cGTACCACACAGGCATTCTTGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGaaaa                                                                                                          <  1:811704/140‑1 (MQ=255)                         tACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACg                                                                                                        >  2:347067/1‑140 (MQ=255)                         tACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACg                                                                                                        <  1:576601/140‑1 (MQ=255)                            cacacaGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTg                                                                                                                 <  2:1219522/128‑1 (MQ=255)                            cacacaGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTg                                                                                                                 >  1:1219522/1‑128 (MQ=255)                              cacaGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCg                                                                                                               >  2:156385/1‑128 (MQ=255)                              cacaGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCg                                                                                                               <  1:156385/128‑1 (MQ=255)                              cacaGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGc                                                                                                   >  1:177332/1‑140 (MQ=255)                                   gCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGt                                                                                              >  1:94735/1‑140 (MQ=255)                                   gCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGt                                                                                              >  1:663252/1‑140 (MQ=255)                                              aGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAg                                                                                   <  2:177332/140‑1 (MQ=255)                                              aGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAg                                                                                   <  2:60999/140‑1 (MQ=255)                                                 ttGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCg                                                                                <  2:94735/140‑1 (MQ=255)                                                 ttGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCg                                                                                <  1:119605/140‑1 (MQ=255)                                                    ccGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCg                                                                             >  2:21803/1‑140 (MQ=255)                                                             tACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCa                                                                    <  2:663252/140‑1 (MQ=255)                                                              aCGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCAt                                                                   >  2:1488127/1‑140 (MQ=255)                                                               cGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATc                                                                  >  2:1322980/1‑140 (MQ=255)                                                               cGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATc                                                                  <  1:1488127/140‑1 (MQ=255)                                                                    gacgacGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCaa                                                             >  2:479802/1‑140 (MQ=255)                                                                     acgacgATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCaaa                                                            >  1:99317/1‑140 (MQ=255)                                                                         cgaTCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCa                                                        >  2:577813/1‑140 (MQ=255)                                                                          gaTCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAc                                                       <  1:1292950/140‑1 (MQ=255)                                                                           aTCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAcc                                                      <  2:37916/140‑1 (MQ=255)                                                                             cgcgCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccgg                                                    <  1:479802/140‑1 (MQ=255)                                                                              gcgcCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggc                                                   >  1:1277489/1‑140 (MQ=255)                                                                              gcgcCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggc                                                   >  2:17767/1‑140 (MQ=255)                                                                              gcgcCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggc                                                   >  1:1370915/1‑140 (MQ=255)                                                                              gcgcCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggc                                                   >  2:149199/1‑140 (MQ=255)                                                                               cgcCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggcc                                                  >  2:122905/1‑140 (MQ=255)                                                                                  cAATGGCTGTATCCGCTCGCTGGAACATGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggcccgg                                               <  1:964896/140‑1 (MQ=255)                                                                                  cAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCAccggcccgg                                               <  1:17767/140‑1 (MQ=255)                                                                                    aTGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCg                                             <  1:524500/140‑1 (MQ=255)                                                                                     tGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGa                                            <  1:1216032/140‑1 (MQ=255)                                                                                          gTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAgtg                                       >  1:1327164/1‑140 (MQ=255)                                                                                             tCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTAc                                    >  2:454835/1‑140 (MQ=255)                                                                                              ccGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  1:15417/140‑1 (MQ=255)                                                                                              ccGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  2:359700/140‑1 (MQ=255)                                                                                              ccGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  1:1443908/140‑1 (MQ=255)                                                                                              ccGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  2:290697/140‑1 (MQ=255)                                                                                              ccGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  2:266720/140‑1 (MQ=255)                                                                                                gctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGaa                                 >  2:1067202/1‑140 (MQ=255)                                                                                                gctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGaa                                 >  1:1303492/1‑140 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCCTCCTCAAATTCACCGGCCCGGCGAAAGTGGACGaaa                                >  2:204249/1‑140 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   >  1:1477929/1‑137 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  1:1235414/137‑1 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   >  2:1235414/1‑137 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACg                                   <  2:1477929/137‑1 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGaaa                                >  2:242971/1‑140 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGaaa                                >  1:1244475/1‑140 (MQ=255)                                                                                                 ctcgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGaaa                                >  1:1379153/1‑140 (MQ=255)                                                                                                  acgctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAg                               >  1:707810/2‑140 (MQ=255)                                                                                                     ctGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCa                            >  2:538282/1‑140 (MQ=255)                                                                                                        gAACACGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGa                         <  2:1327164/140‑1 (MQ=255)                                                                                                          acacGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACg                       >  1:1098631/1‑140 (MQ=255)                                                                                                           cacGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGa                      <  1:604735/140‑1 (MQ=255)                                                                                                           cacGCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGa                      <  2:1244475/140‑1 (MQ=255)                                                                                                              gCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGATGATGc                   >  1:852505/1‑140 (MQ=255)                                                                                                              gCCTACAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATgg                   >  2:258259/1‑139 (MQ=255)                                                                                                                  aCAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACAGCTGCATCGTGAAATCGGCAGGCGTCTACTACAGCATCCTCAAATTTACTGGCCCGGCCAGAGTTTACGAAAACCACGGCGAGGCggga               >  2:482376/1‑138 (MQ=255)                                                                                                                   cAGCAAAGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAg              >  1:497772/1‑140 (MQ=255)                                                                                                                         aGACGGCGGCCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGa        <  2:1098631/140‑1 (MQ=255)                                                                                                                              gcggcCTGGCGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATtctc   >  2:934681/1‑140 (MQ=255)                                                                                                                               cggcCTGGTGGCGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATTCTcg  <  1:482376/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 CCGCGCAGGTCCTGCAGGCATTCGTACCACACAGGCATTCTCGCAAGATTGCCGTTGGGATACGCTGGACGACGATCGCGCCAATGGCTGTATCCGCTCGCTGGAACACGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATTCTCG  >  NC_000913/3954611‑3954876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑