Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,017,388	G→A	G19D (GGC→GAC)	rmuC →	putative recombination limiting protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,388	0	G	A	100.0%	289.3 / NA	81	G19D (GGC→GAC)	rmuC	putative recombination limiting protein RmuC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/37);  total (44/37)

GAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTT  >  NC_000913/4017257‑4017523                                                                                                                                    |                                                                                                                                        gAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGt                                                                                                                                 >  1:283737/1‑140 (MQ=255) gAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGt                                                                                                                                 >  2:901482/1‑140 (MQ=255)  aaaTGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGtt                                                                                                                                <  2:1371173/140‑1 (MQ=255)    aTGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTg                                                                                                                              >  1:613551/1‑140 (MQ=255)     tGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGc                                                                                                                             <  2:221298/140‑1 (MQ=255)       ccTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCa                                                                                                                           >  2:129276/1‑140 (MQ=255)        cTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAg                                                                                                                          <  1:478312/140‑1 (MQ=255)        cTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAg                                                                                                                          <  1:129276/140‑1 (MQ=255)             cAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATc                                                                                                                     <  2:626516/140‑1 (MQ=255)                    aCGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATgc                                                                                                              >  1:1335760/1‑140 (MQ=255)                     cGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATgcg                                                                                                             <  2:1335760/140‑1 (MQ=255)                     cGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATgcg                                                                                                             <  1:303143/140‑1 (MQ=255)                          gTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCgcagca                                                                                                        >  2:57248/1‑140 (MQ=255)                               gCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAg                                                                                                   <  2:1514531/140‑1 (MQ=255)                                cacaATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGc                                                                                                  >  2:20304/1‑140 (MQ=255)                                  caATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCg                                                                                                >  2:403361/1‑140 (MQ=255)                                  caATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCg                                                                                                >  2:927810/1‑140 (MQ=255)                                  caATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCg                                                                                                >  1:791139/1‑140 (MQ=255)                                   aaTTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGa                                                                                               <  1:955274/140‑1 (MQ=255)                                   aaTTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGa                                                                                               <  1:747921/140‑1 (MQ=255)                                   aaTTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGa                                                                                               <  1:371537/140‑1 (MQ=255)                                       cTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCaa                                                                                           <  1:403361/140‑1 (MQ=255)                                          ttttGTGAGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTa                                                                                        >  1:1043066/1‑140 (MQ=255)                                            ttGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGc                                                                                      <  1:708720/140‑1 (MQ=255)                                               tgCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa                                                                                   >  2:475129/1‑140 (MQ=255)                                               tgCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa                                                                                   >  1:15235/1‑140 (MQ=255)                                               tgCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa                                                                                   >  1:1237651/1‑140 (MQ=255)                                               tgCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa                                                                                   >  1:1232474/1‑140 (MQ=255)                                               tgCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa                                                                                   <  1:1147026/140‑1 (MQ=255)                                                    gTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTg                                                                              >  2:30881/1‑140 (MQ=255)                                                     tAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGa                                                                             <  1:1073967/140‑1 (MQ=255)                                                          tgttgCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAgaga                                                                        <  1:927810/140‑1 (MQ=255)                                                            ttgCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATg                                                                      <  1:57248/140‑1 (MQ=255)                                                               cGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTc                                                                   <  2:283737/140‑1 (MQ=255)                                                                 tCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTcgc                                                                 >  2:217817/1‑140 (MQ=255)                                                                 tCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTcgc                                                                 <  2:791139/140‑1 (MQ=255)                                                                 tCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTcgc                                                                 >  1:841039/1‑140 (MQ=255)                                                                  cAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTcgcg                                                                >  1:77213/1‑140 (MQ=255)                                                                      aggCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGt                                                            >  2:816597/1‑140 (MQ=255)                                                                         cGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTaa                                                         >  2:1047242/1‑140 (MQ=255)                                                                           ttGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAgc                                                       >  1:1494958/1‑140 (MQ=255)                                                                                 aTTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCa                                                 <  2:1043066/140‑1 (MQ=255)                                                                                  tttCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCaa                                                >  1:18630/1‑140 (MQ=255)                                                                                   ttCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCaaa                                               >  2:260867/1‑140 (MQ=255)                                                                                     ctcAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGCCGCGGAGTTAAGCGCGGCAAaac                                             >  2:231868/1‑140 (MQ=255)                                                                                          tCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGCCGCGGAGTTAAGCGCGGCAAAAcaacaa                                        <  1:231868/140‑1 (MQ=255)                                                                                             tGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAAtt                                     >  1:851821/1‑140 (MQ=255)                                                                                               gTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTAc                                   >  2:732821/1‑140 (MQ=255)                                                                                                    cGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCaaa                              <  1:732821/140‑1 (MQ=255)                                                                                                    cGCAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCaaa                              >  2:118962/1‑140 (MQ=255)                                                                                                      cAGTTATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGc                            <  1:260867/140‑1 (MQ=255)                                                                                                         ttATTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAg                         >  1:499882/1‑140 (MQ=255)                                                                                                           aTTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       <  1:20304/140‑1 (MQ=255)                                                                                                           aTTGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       <  2:1232474/140‑1 (MQ=255)                                                                                                             tGCGTTGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACt                     <  2:18630/140‑1 (MQ=255)                                                                                                                 ttGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       <  2:935774/134‑1 (MQ=255)                                                                                                                 ttGGTGGGTGTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       >  1:935774/1‑134 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACt                     >  2:83376/1‑134 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACt                     <  1:83376/134‑1 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  2:458892/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  2:986216/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  2:613813/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  2:246171/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  1:1249183/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  1:1437913/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  1:18555/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  1:385160/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  1:941187/1‑140 (MQ=255)                                                                                                                   ggtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTg               >  2:297809/1‑140 (MQ=255)                                                                                                                    gtgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGc              >  1:1464147/1‑140 (MQ=255)                                                                                                                     tgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCa             <  2:505367/140‑1 (MQ=255)                                                                                                                     tgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCa             <  1:613813/140‑1 (MQ=255)                                                                                                                     tgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCa             <  2:1365614/140‑1 (MQ=255)                                                                                                                     tgggtgTGGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCa             <  2:15235/140‑1 (MQ=255)                                                                                                                         tgtgGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       >  1:952515/1‑126 (MQ=255)                                                                                                                         tgtgGCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCa                       <  2:952515/126‑1 (MQ=255)                                                                                                                            ggCAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCg      >  2:1496604/1‑140 (MQ=255)                                                                                                                              cAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGGGCACTGGCGTGCAGAGTGCGAg    <  1:475129/140‑1 (MQ=255)                                                                                                                              cAATTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAg    <  1:30881/140‑1 (MQ=255)                                                                                                                                aTTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGtt  <  2:499882/140‑1 (MQ=255)                                                                                                                                aTTGACTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGtt  <  1:891220/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        GAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGAACGTGAAGAGATGGTCGCGGAGTTAAGCGCGGCAAAACAACAAATTACCCAAAGCGAGCACTGGCGTGCAGAGTGCGAGTT  >  NC_000913/4017257‑4017523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑