Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,101,899	A→G	V70V (GTA→GTG)	kdgT →	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,899	0	A	G	100.0%	264.8 / NA	76	V70V (GTA→GTG)	kdgT	2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/37);  total (39/37)

CACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGG  >  NC_000913/4101765‑4102035                                                                                                                                       |                                                                                                                                         cacaCCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCg                                                                                                                                     <  1:106888/140‑1 (MQ=255)      cTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAAt                                                                                                                                >  1:636520/1‑140 (MQ=255)        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTTAGCGCGACGGGAACGATGCTGCGTAAAGcc                                                                                                                              >  2:932319/1‑140 (MQ=255)        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATcc                                                                                                                              >  1:611585/1‑140 (MQ=255)        tctcGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATcc                                                                                                                              >  1:611109/1‑140 (MQ=255)           cGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGt                                                                                                                           <  2:171674/140‑1 (MQ=255)            gCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTa                                                                                                                          <  1:161395/140‑1 (MQ=255)              cGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTACCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACg                                                                                                                        <  2:581547/140‑1 (MQ=255)                ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCt                                                                                                                      >  2:968425/1‑140 (MQ=255)                ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCt                                                                                                                      <  2:1239802/140‑1 (MQ=255)                ggggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGGACGCt                                                                                                                      >  2:844385/1‑140 (MQ=255)                 gggCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtg                                                                                                                     <  1:1035732/140‑1 (MQ=255)                   gCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggt                                                                                                                   <  1:844385/140‑1 (MQ=255)                   gCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggt                                                                                                                   <  2:189766/140‑1 (MQ=255)                   gCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggt                                                                                                                   <  2:611585/140‑1 (MQ=255)                    cGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggtg                                                                                                                  <  1:1022308/140‑1 (MQ=255)                              ttttGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTaaaa                                                                                                        <  1:1475166/140‑1 (MQ=255)                              ttttGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTaaaa                                                                                                        <  1:28586/140‑1 (MQ=255)                                 tGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggtg                                                                                                                  <  2:1252889/127‑1 (MQ=255)                                 tGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCtggtg                                                                                                                  >  1:1252889/1‑127 (MQ=255)                                  ggATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGc                                                                                                    <  1:1434794/140‑1 (MQ=255)                                      cATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTc                                                                                                <  1:656113/140‑1 (MQ=255)                                        ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                              <  2:523121/140‑1 (MQ=255)                                        ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                              >  2:902004/1‑140 (MQ=255)                                        ttCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTcgc                                                                                              >  2:1029461/1‑140 (MQ=255)                                             cAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtggg                                                                                         <  1:1138005/140‑1 (MQ=255)                                              aaCGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgggt                                                                                        <  1:1151829/140‑1 (MQ=255)                                                cGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCgtgggtgg                                                                                      >  1:1311680/1‑140 (MQ=255)                                                  ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                                                                                                  >  2:1019674/1‑126 (MQ=255)                                                  ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCg                                                                                                  <  1:1019674/126‑1 (MQ=255)                                                  ggATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGtt                                                                                    >  1:1182789/1‑140 (MQ=255)                                                   gatgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTg                                                                                   >  2:933946/1‑140 (MQ=255)                                                     tgatTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCg                                                                                 >  1:1515445/1‑140 (MQ=255)                                                       atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGc                                                                               >  2:852895/1‑140 (MQ=255)                                                       atTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGc                                                                               >  1:976037/1‑140 (MQ=255)                                                        ttACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCa                                                                              <  2:1311680/140‑1 (MQ=255)                                                           ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAtt                                                                           >  2:679691/1‑140 (MQ=255)                                                           ccGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAAtt                                                                           >  1:998892/1‑140 (MQ=255)                                                              gTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGcc                                                                        >  2:735424/1‑140 (MQ=255)                                                                aCGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                                      <  1:852895/140‑1 (MQ=255)                                                                aCGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTc                                                                      <  2:636520/140‑1 (MQ=255)                                                                 cGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTcg                                                                     <  2:1515445/140‑1 (MQ=255)                                                                    tGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGcc                                                                        >  2:1135976/1‑134 (MQ=255)                                                                    tGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGcc                                                                        <  1:1135976/134‑1 (MQ=255)                                                                    tGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGt                                                                  >  2:278470/1‑140 (MQ=255)                                                                      cccATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtat                                                                <  1:34784/140‑1 (MQ=255)                                                                        cATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtatta                                                              <  1:1029461/140‑1 (MQ=255)                                                                        cATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGtatta                                                              >  2:682760/1‑140 (MQ=255)                                                                              ggCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCgg                                                        >  2:970773/1‑140 (MQ=255)                                                                                cGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGaa                                                      >  2:395988/1‑140 (MQ=255)                                                                                  gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                                    <  1:932319/140‑1 (MQ=255)                                                                                  gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                                    >  2:391444/1‑140 (MQ=255)                                                                                  gtgtGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGGAACTAAAATTGCCGTCGCGTGGGAGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                                    >  1:662739/1‑140 (MQ=255)                                                                                     tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATgg                                                 >  2:26610/1‑140 (MQ=255)                                                                                     tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATgg                                                 >  2:814411/1‑140 (MQ=255)                                                                                     tgGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATgg                                                 >  1:448453/1‑140 (MQ=255)                                                                                               gCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                                    >  2:342124/1‑127 (MQ=255)                                                                                               gCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACa                                                    <  1:342124/127‑1 (MQ=255)                                                                                               gCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGtt                                       >  2:483915/1‑140 (MQ=255)                                                                                                 aTGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTgg                                     <  1:391444/140‑1 (MQ=255)                                                                                                  tGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATg                                                  >  1:532050/1‑126 (MQ=255)                                                                                                  tGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATg                                                  <  2:532050/126‑1 (MQ=255)                                                                                                  tGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGa                                    >  2:1156381/1‑140 (MQ=255)                                                                                                  tGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGa                                    >  1:73719/1‑140 (MQ=255)                                                                                                   gggggCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAt                                   <  1:814411/140‑1 (MQ=255)                                                                                                    ggggCGTCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                                  >  2:514171/1‑140 (MQ=255)                                                                                                          tCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGc                            >  1:1441364/1‑140 (MQ=255)                                                                                                          tCAATAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGc                            >  2:812753/1‑140 (MQ=255)                                                                                                             aTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                                  <  1:71782/131‑1 (MQ=255)                                                                                                             aTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGAtt                                  >  2:71782/1‑131 (MQ=255)                                                                                                             aTAAAATTAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCgg                         <  1:933946/140‑1 (MQ=255)                                                                                                                   ttAAGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTc                   <  1:1103720/140‑1 (MQ=255)                                                                                                                     aaGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCaa                 <  2:770065/140‑1 (MQ=255)                                                                                                                      aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGc                            <  1:1152336/128‑1 (MQ=255)                                                                                                                      aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGc                            >  2:1152336/1‑128 (MQ=255)                                                                                                                      aGCGCGACGGGAACGGTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAAc                >  2:375643/1‑140 (MQ=255)                                                                                                                                    ggTGCTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCtggtgg  <  1:679691/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CACACCTTCTCGCCGGGGGCGGGGAAATATTTTGGATCATTCACCAACGGGATGATTACCGGTACGGTGCCCATTCTGGCGGTGTGGTTTTTTTGCATGGGGGCGTCAATAAAATTAAGCGCGACGGGAACGGTACTGCGTAAATCCGGTACGCTGGTGGTAACTAAAATTGCCGTCGCGTGGGTGGTTGCGGCAATTGCCTCGCGTATTATTCCGGAACATGGTGTTGAAGTTGGATTCTTTGCCGGACTTTCAACGCTGGCGCTGGTGG  >  NC_000913/4101765‑4102035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑