Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,112,221	T→C	intergenic (‑7/‑94)	yiiQ ← / → yiiR	DUF1454 domain‑containing protein YiiQ/DUF805 domain‑containing protein YiiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,112,221	0	T	C	100.0%	296.7 / NA	84	intergenic (‑7/‑94)	yiiQ/yiiR	DUF1454 domain‑containing protein YiiQ/DUF805 domain‑containing protein YiiR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/43);  total (41/43)

AGATCGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGT  >  NC_000913/4112084‑4112353                                                                                                                                          |                                                                                                                                     agaTCGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTGACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCAc                                                                                                                                    >  2:940953/1‑140 (MQ=255)    tCGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCtt                                                                                                                                 <  2:1179034/140‑1 (MQ=255)     cGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCttt                                                                                                                                >  2:1239887/1‑140 (MQ=255)     cGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCttt                                                                                                                                >  1:922551/1‑140 (MQ=255)     cGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCttt                                                                                                                                >  2:1158597/1‑140 (MQ=255)         aGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGtt                                                                                                                            <  2:304394/140‑1 (MQ=255)         aGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGtt                                                                                                                            <  2:408036/140‑1 (MQ=255)             ggggCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGa                                                                                                                        >  1:940062/1‑140 (MQ=255)              gggCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGAc                                                                                                                       >  2:877278/1‑140 (MQ=255)               ggCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACt                                                                                                                      >  1:1439287/1‑140 (MQ=255)                 cTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTgg                                                                                                                    >  2:250136/1‑140 (MQ=255)                   ccagccagCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCt                                                                                                                  <  2:1272230/140‑1 (MQ=255)                        cagcagATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGAt                                                                                                             <  1:701605/140‑1 (MQ=255)                          gcagATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTa                                                                                                           <  2:1477344/140‑1 (MQ=255)                                 aGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGat                                                                                                    >  2:1391515/1‑140 (MQ=255)                                 aGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGat                                                                                                    >  1:876452/1‑140 (MQ=255)                                    cgcgGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATc                                                                                                 >  2:660256/1‑140 (MQ=255)                                     gcgGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCt                                                                                                >  2:894975/1‑140 (MQ=255)                                      cgGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTg                                                                                               >  1:1390285/1‑140 (MQ=255)                                       ggTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGt                                                                                              >  2:1033272/1‑140 (MQ=255)                                         tCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGat                                                                                                    <  2:1166500/132‑1 (MQ=255)                                         tCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGat                                                                                                    >  1:1166500/1‑132 (MQ=255)                                         tCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCg                                                                                            <  1:660256/140‑1 (MQ=255)                                           gTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGcc                                                                                          <  1:508778/140‑1 (MQ=255)                                              gCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCtt                                                                                       <  1:1320975/140‑1 (MQ=255)                                                   aTCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaa                                                                                  <  2:291383/140‑1 (MQ=255)                                                   aTCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaa                                                                                  <  1:1033272/140‑1 (MQ=255)                                                   aTCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaa                                                                                  <  2:939038/140‑1 (MQ=255)                                                   aTCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaa                                                                                  <  2:1487266/140‑1 (MQ=255)                                                    tCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaaa                                                                                 >  1:508469/1‑140 (MQ=255)                                                     cTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCaaaa                                                                                <  2:1439287/140‑1 (MQ=255)                                                         tgtTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGt                                                                            >  2:571536/1‑140 (MQ=255)                                                         tgtTCGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCC‑GGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTa                                                                           <  1:899978/140‑1 (MQ=255)                                                          gtTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTa                                                                           <  1:1158597/140‑1 (MQ=255)                                                            ttGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGa                                                                         >  2:374253/1‑140 (MQ=255)                                                             tgtgCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGaa                                                                        <  2:940062/140‑1 (MQ=255)                                                                   tGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTgg                                                                  >  1:1406108/1‑140 (MQ=255)                                                                     cgcCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGaa                                                                >  2:175141/1‑140 (MQ=255)                                                                     cgcCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGaa                                                                <  1:1391515/140‑1 (MQ=255)                                                                       ccTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGaaaa                                                              >  2:1442792/1‑140 (MQ=255)                                                                         tctGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTa                                                                           <  1:707600/125‑1 (MQ=255)                                                                         tctGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTa                                                                           >  2:707600/1‑125 (MQ=255)                                                                                  aCGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAGGGGTAGAATCCTGGAAAAGACAACCATCa                                                   >  1:1152570/1‑140 (MQ=255)                                                                                  aCGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCa                                                   >  2:1488879/1‑140 (MQ=255)                                                                                    ggTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAg                                                 <  1:175141/140‑1 (MQ=255)                                                                                      ttAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGa                                               <  2:565808/140‑1 (MQ=255)                                                                                      ttAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGAGTAGAATCCTGGAAAAGACAACCATCAAGGa                                               <  2:618402/140‑1 (MQ=255)                                                                                       tAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGaa                                              <  1:1442792/140‑1 (MQ=255)                                                                                       tAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGa                                               <  1:370823/139‑1 (MQ=255)                                                                                       tAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGa                                               >  2:370823/1‑139 (MQ=255)                                                                                             aGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACa                                        <  1:940953/140‑1 (MQ=255)                                                                                             aGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACa                                        <  2:922551/140‑1 (MQ=255)                                                                                               aaCATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATg                                      >  2:282778/1‑140 (MQ=255)                                                                                                aCATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGa                                     <  1:1099727/140‑1 (MQ=255)                                                                                                   tAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCa                                  <  2:876452/140‑1 (MQ=255)                                                                                                    aaGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCAt                                 >  2:1516652/1‑140 (MQ=255)                                                                                                    aaGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTA‑GACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATa                                >  2:765324/1‑140 (MQ=255)                                                                                                     agagAAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATa                                <  1:282778/140‑1 (MQ=255)                                                                                                        gaAAAAACAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTA‑GACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            <  1:765324/140‑1 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGa                                     <  1:837751/131‑1 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGa                                     >  2:837751/1‑131 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            <  1:894975/140‑1 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            >  1:305118/1‑140 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            <  1:250136/140‑1 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            <  1:1516652/140‑1 (MQ=255)                                                                                                         aaaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGc                            >  2:1378947/1‑140 (MQ=255)                                                                                                          aaaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCa                           <  1:1239887/140‑1 (MQ=255)                                                                                                           aaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATATAGCaa                          >  1:400870/1‑140 (MQ=255)                                                                                                           aaaaCAGCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCaa                          >  1:1415007/1‑140 (MQ=255)                                                                                                                 gCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATa                                <  2:336855/128‑1 (MQ=255)                                                                                                                 gCGTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATa                                >  1:336855/1‑128 (MQ=255)                                                                                                                   gTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                  <  1:1075119/140‑1 (MQ=255)                                                                                                                   gTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                  <  2:30967/140‑1 (MQ=255)                                                                                                                   gTACACCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTAtt                  <  1:1450645/140‑1 (MQ=255)                                                                                                                       acCCTGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCa              >  2:836813/1‑140 (MQ=255)                                                                                                                           tGGCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTa          <  2:1415007/140‑1 (MQ=255)                                                                                                                            ggCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTGCATGACCATACAGCAATGGTTATTCTCATTTaa         >  2:1209890/1‑140 (MQ=255)                                                                                                                            ggCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTaa         >  2:764716/1‑140 (MQ=255)                                                                                                                            ggCTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTaa         <  2:508469/140‑1 (MQ=255)                                                                                                                              cTTCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAg       >  2:880104/1‑140 (MQ=255)                                                                                                                               ttCATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAgg      <  1:1378947/140‑1 (MQ=255)                                                                                                                                 cATTGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGc    >  1:275631/1‑140 (MQ=255)                                                                                                                                   ttGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGt  >  2:72273/1‑140 (MQ=255)                                                                                                                                   ttGACAGCACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGt  >  2:1062263/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AGATCGAAAGTAGGGGCTCCAGCCAGCAGATAAGGCGCGGTCGTTGCCGTATCTGGTGTTTGTGCATGCGCCTCTGTTGTAACGGTTAATGCAGAACATAAGAGAAAAAACAGCGTACACCCTGGCTTCATTGACAGTACCTTTTGTTATGACTGGCTCCGATTAAAACGATATCTGTCGCCCTTGTCAAAAGGGTAGAATCCTGGAAAAGACAACCATCAAGGAACTTACATGACCATACAGCAATGGTTATTCTCATTTAAAGGGCGT  >  NC_000913/4112084‑4112353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑