Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,124	A→G	V286A (GTC→GCC)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,124	0	A	G	100.0%	268.6 / NA	76	V286A (GTC→GCC)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/30);  total (46/30)

GAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGG  >  NC_000913/4309989‑4310252                                                                                                                                        |                                                                                                                                 gAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCt                                                                                                                              <  2:567793/140‑1 (MQ=255)  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTa                                                                                                                             >  2:477146/1‑140 (MQ=255)  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTa                                                                                                                             >  2:428201/1‑140 (MQ=255)  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTa                                                                                                                             >  2:1503436/1‑140 (MQ=255)  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTa                                                                                                                             >  2:764162/1‑140 (MQ=255)  aaCGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTa                                                                                                                             >  1:1513223/1‑140 (MQ=255)    cGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTaaa                                                                                                                           >  2:615443/1‑140 (MQ=255)        gagagaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATgag                                                                                                                       >  2:1473879/1‑140 (MQ=255)        gagagaTATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATgag                                                                                                                       >  1:395089/1‑140 (MQ=255)               atCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATccc                                                                                                                >  1:1194928/1‑140 (MQ=255)               atCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATccc                                                                                                                >  1:524755/1‑140 (MQ=255)               atCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGACAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATccc                                                                                                                >  2:486563/1‑140 (MQ=255)                 cTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCgg                                                                                                              <  2:239989/140‑1 (MQ=255)                         ttAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCttttt                                                                                                      <  1:612230/140‑1 (MQ=255)                         ttAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCttttt                                                                                                      <  1:616561/140‑1 (MQ=255)                          tAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCtttttt                                                                                                     <  2:1194928/140‑1 (MQ=255)                          tAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGACAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCtttttt                                                                                                     <  1:486563/140‑1 (MQ=255)                              aCGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGt                                                                                                 <  1:764162/140‑1 (MQ=255)                                 gATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCAc                                                                                              >  1:1222809/1‑140 (MQ=255)                                  aTTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACg                                                                                             <  1:58700/140‑1 (MQ=255)                                         cGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTg                                                                                      <  1:428201/140‑1 (MQ=255)                                           ccAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGAc                                                                                    >  1:232733/1‑140 (MQ=255)                                               cgcgTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT‑CCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTcc                                                                               >  2:1417936/1‑140 (MQ=255)                                                 cgTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCg                                                                              <  2:232733/140‑1 (MQ=255)                                                  gTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGc                                                                             <  1:530062/140‑1 (MQ=255)                                                        ccACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCT‑CCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCaa                                                                      <  1:1417936/140‑1 (MQ=255)                                                            gCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCaaaaa                                                                   >  1:807345/1‑140 (MQ=255)                                                                   aGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTc                                                            <  2:176635/140‑1 (MQ=255)                                                                      cAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTg                                                         >  2:843122/1‑140 (MQ=255)                                                                        aTTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTgcg                                                       >  2:1391614/1‑140 (MQ=255)                                                                          tcatcaGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCa                                                     <  1:1503436/140‑1 (MQ=255)                                                                                 ttcagtACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGc                                              >  2:1364707/1‑140 (MQ=255)                                                                                 ttcagtACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGc                                              <  2:578402/140‑1 (MQ=255)                                                                                 ttcagtACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGc                                              >  2:1489278/1‑140 (MQ=255)                                                                                    agtACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGa                                           >  2:419032/1‑140 (MQ=255)                                                                                      tACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACa                                         <  1:1391614/140‑1 (MQ=255)                                                                                      tACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACa                                         <  1:539708/140‑1 (MQ=255)                                                                                      tACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACa                                         <  1:477146/140‑1 (MQ=255)                                                                                        cGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACAtt                                       >  1:1054196/1‑140 (MQ=255)                                                                                           ccGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATtctc                                    >  1:1190788/1‑140 (MQ=255)                                                                                                aCCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGt                                                 >  1:30559/1‑122 (MQ=255)                                                                                                aCCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGt                                                 <  2:30559/122‑1 (MQ=255)                                                                                                aCCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCa                               >  1:833984/1‑140 (MQ=255)                                                                                                aCCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCa                               <  1:1364707/140‑1 (MQ=255)                                                                                                    aCCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATcgc                           >  2:1232431/1‑140 (MQ=255)                                                                                                    aCCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATcgc                           >  1:1491954/1‑140 (MQ=255)                                                                                                    aCCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATcgc                           >  2:1434872/1‑140 (MQ=255)                                                                                                    aCCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATcgc                           >  2:549218/1‑140 (MQ=255)                                                                                                     ccAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATcgcg                          >  1:493735/1‑140 (MQ=255)                                                                                                         tCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTaa                      >  2:1201972/1‑140 (MQ=255)                                                                                                         tCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTaa                      >  1:421859/1‑140 (MQ=255)                                                                                                         tCCGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTaa                      >  1:461818/1‑140 (MQ=255)                                                                                                           cGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTaaaa                    <  2:1513223/140‑1 (MQ=255)                                                                                                           cGGCAAAGCCTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTaaaa                    <  1:615443/140‑1 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTg            >  2:234718/1‑140 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTg            >  1:169475/1‑140 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTg            >  2:678271/1‑140 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTg            >  2:585299/1‑140 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGGTGCTGACATTCTCCTTCATCGCGTTAAAACGGttttt            >  2:1396931/1‑139 (MQ=255)                                                                                                                   ccTAATATTTCTCCCCGCCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTg            >  2:707238/1‑140 (MQ=255)                                                                                                                       atatTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGt        >  1:163848/1‑140 (MQ=255)                                                                                                                        tatTTCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGtt       <  2:807345/140‑1 (MQ=255)                                                                                                                          tttCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     <  1:419032/140‑1 (MQ=255)                                                                                                                          tttCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     <  2:694153/140‑1 (MQ=255)                                                                                                                           ttCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcgc    <  1:234718/140‑1 (MQ=255)                                                                                                                           ttCTCCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcgc    <  1:843122/140‑1 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTTCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCgg  >  1:1174101/1‑140 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     >  1:1093252/1‑137 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     >  2:459814/1‑137 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     <  2:1093252/137‑1 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     <  1:459814/137‑1 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCgg  >  2:517976/1‑140 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCgg  >  1:4678/1‑140 (MQ=255)                                                                                                                             ctcCCCGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCgg  >  2:879252/1‑140 (MQ=255)                                                                                                                                  cGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     <  2:73998/132‑1 (MQ=255)                                                                                                                                  cGGCAGGCGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTcg     >  1:73998/1‑132 (MQ=255)                                                                                                                                        |                                                                                                                                 GAACGTGGAGAGATATCTTTGCCATTAAGACGGATTTCTCCGCCAGCGCGTTTATCCACGCCAAACAGACAATTCATCAGTTCAGTACGTCCGGAACCGACCAGTCCGGCAAAGCCTAATATTTCTCCCCGGCAGACGCTAAATGAGATATCCCGGACCTTTTTTCTGTCACGACTGGTGACGTTCCGCACCTCAAAAACCGTTTCGTGCGCAAGGTTGCTGACATTCTCCTTCATCGCGTTAAAACGGTTTTGCAGTTCGCGG  >  NC_000913/4309989‑4310252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑