Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,483	T→C	intergenic (+350/+207)	yjhV → / ← fecE	KpLE2 phage‑like element; putative uncharacterized protein YjhV/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,483	0	T	C	100.0%	258.8 / NA	73	intergenic (+350/+207)	yjhV/fecE	KpLE2 phage‑like element; putative uncharacterized protein YjhV/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/39);  total (34/39)

TGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCC  >  NC_000913/4510352‑4510617                                                                                                                                    |                                                                                                                                       tGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGa                                                                                                                                <  1:1365359/140‑1 (MQ=255)  gATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGAc                                                                                                                               <  2:153704/140‑1 (MQ=255)   aTCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACt                                                                                                                              <  1:16741/140‑1 (MQ=255)               aaaTCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGcc                                                                                                                  <  1:560297/140‑1 (MQ=255)               aaaTCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGcc                                                                                                                  <  1:673970/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCCCGACTGGTTTGAAAGCCGCGcc                                                                                                             <  2:1076105/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  2:237975/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  1:983130/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  1:588765/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  2:517977/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  1:674850/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  1:1049557/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACCGGTTTGAAAGCCGCGcc                                                                                                             <  2:573926/140‑1 (MQ=255)                    tAAACTTAGTCATGTCACGTTCTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGcc                                                                                                             <  1:1129072/140‑1 (MQ=255)                       aCTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCaa                                                                                                          >  2:60614/1‑140 (MQ=255)                       aCTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCaa                                                                                                          <  1:60614/140‑1 (MQ=255)                         ttAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGcc                                                                                                                  >  1:523830/1‑130 (MQ=255)                         ttAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGcc                                                                                                                  <  2:523830/130‑1 (MQ=255)                                tGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACa                                                                                                 >  2:119622/1‑140 (MQ=255)                                   cACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGct                                                                                              <  1:1015322/140‑1 (MQ=255)                                           tGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTg                                                                                      <  1:1219743/140‑1 (MQ=255)                                               tttCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGtt                                                                                  <  2:548323/140‑1 (MQ=255)                                               tttCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGtt                                                                                  <  1:561700/140‑1 (MQ=255)                                                  cTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGAt                                                                               >  1:1307436/1‑140 (MQ=255)                                                          ttAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAAcc                                                                       <  2:643977/140‑1 (MQ=255)                                                            aaCTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTg                                                                     >  1:574724/1‑140 (MQ=255)                                                             aCTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGt                                                                    <  2:574724/140‑1 (MQ=255)                                                             aCTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGt                                                                    <  2:1307436/140‑1 (MQ=255)                                                                 cgcgTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGc                                                                >  1:622504/1‑140 (MQ=255)                                                                             tGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCg                                                    <  1:998459/140‑1 (MQ=255)                                                                              gCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCgg                                                   >  2:1199510/1‑140 (MQ=255)                                                                                  tAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATgg                                               >  1:1214767/1‑140 (MQ=255)                                                                                      ggTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCAGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGa                                           <  2:1214767/140‑1 (MQ=255)                                                                                       gTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGAt                                          <  1:427378/140‑1 (MQ=255)                                                                                       gTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGAt                                          <  1:119622/140‑1 (MQ=255)                                                                                         tataCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGa                                           <  1:1356639/137‑1 (MQ=255)                                                                                         tataCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGa                                           >  2:1356639/1‑137 (MQ=255)                                                                                         tataCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGa                                        >  2:114131/1‑140 (MQ=255)                                                                                           taCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGt                                      >  1:1071281/1‑140 (MQ=255)                                                                                            aCAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTa                                     >  1:117102/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGAc                                             >  1:1157296/1‑128 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGAc                                             <  2:1157296/128‑1 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGAt                                          <  2:1339256/131‑1 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGAt                                          >  1:1339256/1‑131 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAga                                      >  1:978275/1‑134 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAga                                      <  2:978275/135‑2 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  2:488744/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  2:131498/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 <  2:622504/140‑1 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  2:564892/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  1:1034032/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  2:1106158/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  1:1337443/1‑140 (MQ=255)                                                                                                aaaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTgg                                 >  2:1154174/1‑140 (MQ=255)                                                                                                 aaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGt                                >  1:1309205/1‑140 (MQ=255)                                                                                                 aaTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGt                                >  1:1357174/1‑140 (MQ=255)                                                                                                  aTGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTa                               <  1:131498/140‑1 (MQ=255)                                                                                                     tCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGGAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATc                            >  1:396528/1‑140 (MQ=255)                                                                                                     tCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAAta                            >  1:348419/1‑139 (MQ=255)                                                                                                     tCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATc                            >  2:1134706/1‑140 (MQ=255)                                                                                                     tCCGTTAAGCAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATc                            >  2:729605/1‑140 (MQ=255)                                                                                                              cAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCaga                     <  2:701539/138‑1 (MQ=255)                                                                                                              cAGAGTTCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCaga                     >  1:701539/1‑138 (MQ=255)                                                                                                                   ttCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                       >  1:404615/1‑131 (MQ=255)                                                                                                                   ttCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCa                       <  2:404615/131‑1 (MQ=255)                                                                                                                   ttCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGa              >  2:520034/1‑140 (MQ=255)                                                                                                                    tCAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaa             <  1:425234/140‑1 (MQ=255)                                                                                                                     cAAAATTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaaa            <  2:1357174/140‑1 (MQ=255)                                                                                                                      aaaaTTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaaaa           >  1:1075169/1‑140 (MQ=255)                                                                                                                      aaaaTTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaa             <  1:964459/138‑1 (MQ=255)                                                                                                                      aaaaTTGATTGCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGaa             >  2:964459/1‑138 (MQ=255)                                                                                                                           tgattgCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCa      >  1:309705/1‑140 (MQ=255)                                                                                                                              ttgCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGcc   <  1:520034/140‑1 (MQ=255)                                                                                                                              ttgCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGcc   <  2:1033788/140‑1 (MQ=255)                                                                                                                               tgCCGCGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGccc  <  1:488744/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTGATCACGACTGGTTTGAAAGCCGCGCCCAAGCCTGTACAGCTCTGGTTTGCGTTGATTATGAACCTGTCAGCCTAAAGCAAGCGGATGGACGATGAGTATTGGTAATCTTTCAGAGTCCGGAAAAGTTCAGCCC  >  NC_000913/4510352‑4510617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑