Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,048	A→G	intergenic (+51/+3)	thiI → / ← yajL	tRNA uridine 4‑sulfurtransferase/protein/nucleic acid deglycase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,048	0	A	G	100.0%	143.0 / NA	41	intergenic (+51/+3)	thiI/yajL	tRNA uridine 4‑sulfurtransferase/protein/nucleic acid deglycase 3
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/27);  total (14/27)

TGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTC  >  NC_000913/442911‑443183                                                                                                                                          |                                                                                                                                        tggtgTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGgca                                                                                                                                       <  2:1376826/140‑1 (MQ=255)      tgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCactact                                                                                                                                  <  2:1391855/140‑1 (MQ=255)        aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCg                                                                                                                                >  2:785419/1‑140 (MQ=255)        aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCg                                                                                                                                >  2:1267432/1‑140 (MQ=255)           gcgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGtaa                                                                                                                             <  2:1177039/140‑1 (MQ=255)            cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGtaat                                                                                                                            <  2:686751/140‑1 (MQ=255)            cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGtaat                                                                                                                            <  2:1063335/140‑1 (MQ=255)            cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGtaat                                                                                                                            <  1:785419/140‑1 (MQ=255)                           tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTg                                                                                                             <  2:170444/140‑1 (MQ=255)                           tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTg                                                                                                             <  1:85751/140‑1 (MQ=255)                           tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTg                                                                                                             <  1:51819/140‑1 (MQ=255)                                   cgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATc                                                                                                     >  2:959298/1‑140 (MQ=255)                                     ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                   <  2:735892/140‑1 (MQ=255)                                              cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                          >  2:514069/1‑140 (MQ=255)                                               gcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                                                                                         >  1:14176/1‑140 (MQ=255)                                                cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                                                                                            >  2:497514/1‑136 (MQ=255)                                                cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgt                                                                                            <  1:497514/136‑1 (MQ=255)                                                cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                        >  2:1115542/1‑140 (MQ=255)                                                   gCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCa                                                                                     <  2:14176/140‑1 (MQ=255)                                                    cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                                                                                    >  2:1283465/1‑140 (MQ=255)                                                             aaCAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGc                                                                           <  1:1506590/140‑1 (MQ=255)                                                                  tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                      >  2:188599/1‑140 (MQ=255)                                                                   gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                     <  1:188599/140‑1 (MQ=255)                                                                           atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaa                                                             <  1:959298/140‑1 (MQ=255)                                                                                    gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                    <  1:1509208/140‑1 (MQ=255)                                                                                         ttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                               <  2:236441/140‑1 (MQ=255)                                                                                              gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                          >  2:832100/1‑140 (MQ=255)                                                                                                     cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                   <  1:1010341/140‑1 (MQ=255)                                                                                                          cATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                              <  1:832100/140‑1 (MQ=255)                                                                                                          cATCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                              >  2:17406/1‑140 (MQ=255)                                                                                                            tCTGGTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                            <  1:16414/140‑1 (MQ=255)                                                                                                               ggTCAGATGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAg                         <  1:1516312/140‑1 (MQ=255)                                                                                                                      tGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  <  2:670477/140‑1 (MQ=255)                                                                                                                      tGCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  <  1:1115542/140‑1 (MQ=255)                                                                                                                       gCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                 <  1:1283465/140‑1 (MQ=255)                                                                                                                       gCGACGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                 <  1:1267432/140‑1 (MQ=255)                                                                                                                           cGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  <  2:145694/135‑1 (MQ=255)                                                                                                                           cGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  <  2:614280/135‑1 (MQ=255)                                                                                                                           cGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  >  1:145694/1‑135 (MQ=255)                                                                                                                           cGTTTGCCGCATCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                  >  1:614280/1‑135 (MQ=255)                                                                                                                                     aTCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt   <  2:29452/140‑1 (MQ=255)                                                                                                                                      tCCGGCACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTc  >  1:948490/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGTGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTC  >  NC_000913/442911‑443183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑