Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	493,593	T→C	L501P (CTG→CCG)	dnaX →	DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	493,593	0	T	C	100.0%	143.2 / NA	42	L501P (CTG→CCG)	dnaX	DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/23);  total (19/23)

CGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCT  >  NC_000913/493459‑493721                                                                                                                                       |                                                                                                                                 cgTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAAc                                                                                                                             >  1:208168/1‑140 (MQ=255)        gcgcGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGaaaa                                                                                                                      >  1:399811/1‑140 (MQ=255)                                     aGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCgg                                                                                         <  1:11723/140‑1 (MQ=255)                                      gcgcCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGc                                                                                        <  1:1255929/140‑1 (MQ=255)                                      gcgcCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGc                                                                                        <  2:105756/140‑1 (MQ=255)                                      gcgcCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGc                                                                                        <  1:1476298/140‑1 (MQ=255)                                                      aagCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAgcgc                                                                        <  2:566424/140‑1 (MQ=255)                                                           tATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGAccc                                                                   >  1:268300/1‑140 (MQ=255)                                                               gCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTgg                                                               >  1:818788/1‑140 (MQ=255)                                                                cTGGAAGGCGGCCACTCCGGTGAGGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTggg                                                              <  1:1360986/140‑1 (MQ=255)                                                                cTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTggg                                                              <  2:882313/140‑1 (MQ=255)                                                                cTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTggg                                                              <  2:1265219/140‑1 (MQ=255)                                                                   gAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGggcgg                                                           >  2:1306570/1‑140 (MQ=255)                                                                   gAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGggcgg                                                           >  1:455777/1‑140 (MQ=255)                                                                     aGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGggcggcc                                                         >  2:752378/1‑139 (MQ=255)                                                                        cGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAg                                                      >  1:353872/1‑140 (MQ=255)                                                                         gACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAgg                                                     >  1:1051584/1‑140 (MQ=255)                                                                                      aTGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTc                                        <  2:736875/140‑1 (MQ=255)                                                                                       tGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCg                                       >  1:1324735/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  2:471792/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  2:569313/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  1:271336/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  1:398872/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  1:1308734/1‑140 (MQ=255)                                                                                         cagcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACACCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCt                                     >  2:426378/1‑140 (MQ=255)                                                                                           gcaAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTAc                                   <  2:399811/140‑1 (MQ=255)                                                                                              aaaaGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCaa                                >  1:1028766/1‑140 (MQ=255)                                                                                                          cGCCACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAAc                    >  1:816837/1‑140 (MQ=255)                                                                                                            ccACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAg                  <  2:1028766/140‑1 (MQ=255)                                                                                                             cACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCTGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAgg                 <  1:1468965/140‑1 (MQ=255)                                                                                                             cACGCCGAAGGCGCTGAAAAAAGCGCCGGAACATGAAAAAACACCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAgg                 <  1:426378/140‑1 (MQ=255)                                                                                                                          cTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  2:816837/140‑1 (MQ=255)                                                                                                                          cTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  2:818788/140‑1 (MQ=255)                                                                                                                          cTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  1:569313/140‑1 (MQ=255)                                                                                                                          cTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  1:815519/140‑1 (MQ=255)                                                                                                                          cTGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  1:761255/140‑1 (MQ=255)                                                                                                                           tGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    >  2:1436587/1‑139 (MQ=255)                                                                                                                           tGAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGc    <  1:1436587/139‑1 (MQ=255)                                                                                                                            gAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCt  <  2:290172/140‑1 (MQ=255)                                                                                                                            gAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCt  <  2:1247169/140‑1 (MQ=255)                                                                                                                            gAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCt  <  2:1051584/140‑1 (MQ=255)                                                                                                                            gAAAAAAGCGCCGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCt  <  1:752378/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 CGTTCAGGCGCGTCCGGTGCCATCGGCGCTGGAAAAAGCGCCAGCCAAAAAAGAAGCGTATCGCTGGAAGGCGACCACTCCGGTGATGCAGCAAAAAGAAGTGGTCGCCACGCCGAAGGCGCTGAAAAAAGCGCTGGAACATGAAAAAACGCCGGAACTGGCGGCGAAGCTAGCGGCAGAAGCCATTGAGCGCGACCCGTGGGCGGCACAGGTGAGCCAACTTTCGCTACCAAAACTGGTCGAACAGGTGGCGTTAAATGCCT  >  NC_000913/493459‑493721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑