Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	530,868	A→G	L120P (CTG→CCG)	selU ←	tRNA 2‑selenouridine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	530,868	0	A	G	100.0%	303.0 / NA	85	L120P (CTG→CCG)	selU	tRNA 2‑selenouridine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/40);  total (45/40)

CACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGAC  >  NC_000913/530731‑531000                                                                                                                                          |                                                                                                                                     cacCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGc                                                                                                                                    >  2:398891/1‑140 (MQ=255) cacCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGc                                                                                                                                    >  2:1515472/1‑140 (MQ=255) cacCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGc                                                                                                                                    >  2:1456426/1‑140 (MQ=255) cacCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGc                                                                                                                                    >  1:959718/1‑140 (MQ=255)  acCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCg                                                                                                                                   <  1:1007158/140‑1 (MQ=255)    cGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGa                                                                                                                                 <  2:135451/140‑1 (MQ=255)      ttCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATa                                                                                                                               >  2:877885/1‑140 (MQ=255)      ttCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATa                                                                                                                               >  2:1357879/1‑140 (MQ=255)      ttCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATa                                                                                                                               >  2:1241313/1‑140 (MQ=255)      ttCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATa                                                                                                                               >  1:396109/1‑140 (MQ=255)      ttCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCCTATAACCGCCTTCCACCGGCGGATa                                                                                                                               >  2:93836/1‑140 (MQ=255)      tccGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGAACAAACGCCCATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGGCGCCTGAATCGCGGGCTGGCGCAGTGCCTTATAACAGCCTTCCACCGGCGGATa                                                                                                                               >  2:139338/3‑140 (MQ=255)        cGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATaat                                                                                                                             <  1:330752/140‑1 (MQ=255)           cTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATaatcaa                                                                                                                          >  2:436603/1‑140 (MQ=255)              ttGCTGCACTAACAGCTTTTTCCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATcc                                                                                                                       <  2:625683/140‑1 (MQ=255)               tgctgcACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATccc                                                                                                                      <  2:959718/140‑1 (MQ=255)                 ctgcACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCg                                                                                                                    <  2:558063/140‑1 (MQ=255)                  tgcACTAACAGCGTTTTCCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGc                                                                                                                   <  2:655365/140‑1 (MQ=255)                  tgcACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGc                                                                                                                   <  1:876538/140‑1 (MQ=255)                  tgcACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGc                                                                                                                   <  1:79008/140‑1 (MQ=255)                  tgcACAAACAGCGTTTTCCCGCTGCCGGTACAACCGCCAATCTGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGc                                                                                                                   <  1:247054/140‑1 (MQ=255)                     aCTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCtgc                                                                                                                >  1:898684/1‑140 (MQ=255)                           aGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCaa                                                                                                          >  1:745390/1‑140 (MQ=255)                              gTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCa                                                                                                       <  1:436603/140‑1 (MQ=255)                                 ttACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCt                                                                                                    >  2:1152622/1‑140 (MQ=255)                                  tACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCtt                                                                                                   <  2:396109/140‑1 (MQ=255)                                  tACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCtt                                                                                                   <  1:877885/140‑1 (MQ=255)                                    ccGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTg                                                                                                 <  1:1098842/140‑1 (MQ=255)                                    ccGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTg                                                                                                 <  1:1515472/140‑1 (MQ=255)                                    ccGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTg                                                                                                 <  1:1456426/140‑1 (MQ=255)                                      gCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  1:535556/140‑1 (MQ=255)                                      gCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  2:745390/140‑1 (MQ=255)                                      gCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  1:1357879/140‑1 (MQ=255)                                      gCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  1:1002131/140‑1 (MQ=255)                                       cTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               >  2:238794/1‑139 (MQ=255)                                       cTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  1:238794/139‑1 (MQ=255)                                        tGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGcaca                                                                                             <  1:1152622/140‑1 (MQ=255)                                         gCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACaa                                                                                            >  2:482925/1‑140 (MQ=255)                                         gCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACaa                                                                                            <  2:1460499/140‑1 (MQ=255)                                         gCCGGAACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCGATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACaa                                                                                            <  1:1108226/140‑1 (MQ=255)                                            ggTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCttt                                                                                                  <  1:1176045/131‑1 (MQ=255)                                            ggTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCttt                                                                                                  >  2:1176045/1‑131 (MQ=255)                                                    cGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               <  1:165642/126‑1 (MQ=255)                                                    cGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                                               >  2:165642/1‑126 (MQ=255)                                                    cGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAgcgc                                                                                 >  2:290837/1‑140 (MQ=255)                                                      ccAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg                                                                               >  2:1215159/1‑140 (MQ=255)                                                        aaTCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGAc                                                                             >  1:1444426/1‑140 (MQ=255)                                                           cAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGc                                                                          >  2:944467/1‑140 (MQ=255)                                                              cACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCAc                                                                       <  2:1444426/140‑1 (MQ=255)                                                                 tATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACggg                                                                    <  1:1144439/140‑1 (MQ=255)                                                                   tCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGgcg                                                                  >  2:54310/1‑140 (MQ=255)                                                                       tttttGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgcagc                                                              <  1:944467/140‑1 (MQ=255)                                                                           tgtgCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCagag                                                          >  2:1514338/1‑140 (MQ=255)                                                                             tgCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGgcg                                                                  >  1:884688/1‑130 (MQ=255)                                                                             tgCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGgcg                                                                  <  2:884688/130‑1 (MQ=255)                                                                             tgCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCCTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGaa                                                        <  1:93836/140‑1 (MQ=255)                                                                              gCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAAt                                                       <  1:1215159/140‑1 (MQ=255)                                                                               ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGGCCGCCACGGGCgca                                                                <  2:1074655/130‑1 (MQ=255)                                                                               ccAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgca                                                                >  1:1074655/1‑130 (MQ=255)                                                                                 aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCGGAGAATAcc                                                    >  2:728003/1‑140 (MQ=255)                                                                                 aGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATAcc                                                    >  2:477223/1‑140 (MQ=255)                                                                                            cGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGAtttt                                         >  2:1089985/1‑140 (MQ=255)                                                                                             gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCTACGGGCGCAGCAGAGAATACCTTGCGGATTTTg                                        >  1:69102/1‑140 (MQ=255)                                                                                             gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTg                                        >  2:811720/1‑140 (MQ=255)                                                                                             gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTg                                        >  1:59884/1‑140 (MQ=255)                                                                                             gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTg                                        >  2:1056668/1‑140 (MQ=255)                                                                                             gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTg                                        >  1:1432901/1‑140 (MQ=255)                                                                                                tGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAg                                     >  1:815372/1‑140 (MQ=255)                                                                                                   aTCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCa                                  <  2:765834/140‑1 (MQ=255)                                                                                                       cgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGGTAATCAATCCCCGCCGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCCCGGGCGCCGCAGAGAATACCTTGCGGGTTTTTCAGGCACGcc                              >  2:1242129/1‑139 (MQ=255)                                                                                                            tGGCGCAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCg                         >  2:1470129/1‑140 (MQ=255)                                                                                                              gcgcAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCACCCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGcc                       <  2:567150/140‑1 (MQ=255)                                                                                                                 cAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCGGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAgg                    <  1:728003/140‑1 (MQ=255)                                                                                                                 cAGTGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAgg                    <  1:1514338/140‑1 (MQ=255)                                                                                                                    tGCCTTATAACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGt                 >  2:1461253/1‑140 (MQ=255)                                                                                                                         tataACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGcc                       >  1:944642/1‑129 (MQ=255)                                                                                                                         tataACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGcc                       <  2:944642/129‑1 (MQ=255)                                                                                                                           taACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATgcg          <  2:815372/140‑1 (MQ=255)                                                                                                                           taACCGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATgcg          <  1:1089985/140‑1 (MQ=255)                                                                                                                              ccGCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCgctg       >  1:231117/1‑140 (MQ=255)                                                                                                                                gCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCgctgct     >  2:519039/1‑140 (MQ=255)                                                                                                                                gCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCgctgct     >  1:113878/1‑140 (MQ=255)                                                                                                                                gCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCgctgct     >  1:1143691/1‑140 (MQ=255)                                                                                                                                gCCTTCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCgctgct     >  1:1400724/1‑140 (MQ=255)                                                                                                                                   ttCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGAc  >  1:589508/1‑140 (MQ=255)                                                                                                                                   ttCCACCGGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGAc  >  2:935101/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CACCGTTCGGCTGTTGCTGCACTAACAGCGTTTTACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCACGCTGCCCGCCAGGCGTCCATGCGCTGCTGAC  >  NC_000913/530731‑531000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑