Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	39,897	A→G	Y164H (TAC→CAC)	caiA ←	crotonobetainyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	39,897	0	A	G	100.0%	276.0 / NA	78	Y164H (TAC→CAC)	caiA	crotonobetainyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/38);  total (40/38)

TCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTACGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTGAGTTCCAC  >  NC_000913/39761‑40030                                                                                                                                          |                                                                                                                                      tCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCgg                                                                                                                                    <  2:1486965/140‑1 (MQ=255)       cagcTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAGCCCCCGTCGCGCGCCATCACCACGATGTGCGGGgtgt                                                                                                                               <  1:163015/140‑1 (MQ=255)         gcTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAgg                                                                                                                            <  2:394547/140‑1 (MQ=255)            aTCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGgcgc                                                                                                                         >  1:1022143/1‑140 (MQ=255)                aTACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCgctgct                                                                                                                     <  1:195659/140‑1 (MQ=255)                aTACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCgctgct                                                                                                                     <  2:1022143/140‑1 (MQ=255)                       gACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaata                                                                                                              >  1:202911/1‑140 (MQ=255)                       gACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaata                                                                                                              >  2:492445/1‑140 (MQ=255)                       gACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaata                                                                                                              >  2:392149/1‑140 (MQ=255)                       gACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaata                                                                                                              >  1:150156/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGCTGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  2:551449/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  2:345166/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  2:1042696/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  2:214375/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  2:1388109/1‑140 (MQ=255)                        aCCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGtaataa                                                                                                             >  1:842493/1‑140 (MQ=255)                         ccGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATaaa                                                                                                            <  2:101141/140‑1 (MQ=255)                         ccGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATaaa                                                                                                            <  1:345166/140‑1 (MQ=255)                         ccGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATaaa                                                                                                            <  1:492445/140‑1 (MQ=255)                          cGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATaaaa                                                                                                           <  1:428714/140‑1 (MQ=255)                           gAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAac                                                                                                          >  1:556597/1‑140 (MQ=255)                            aGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAaca                                                                                                         <  1:214375/140‑1 (MQ=255)                            aGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAaca                                                                                                         <  1:283420/140‑1 (MQ=255)                            aGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAaca                                                                                                         <  1:1042696/140‑1 (MQ=255)                                  tCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtact                                                                                                   >  2:173660/1‑140 (MQ=255)                                  tCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtact                                                                                                   <  1:1388109/140‑1 (MQ=255)                                  tCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtact                                                                                                   <  1:26692/140‑1 (MQ=255)                                  tCAAGTTTGGGCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtact                                                                                                   >  2:752369/1‑140 (MQ=255)                                       tttGGTCGCTTTGATGCACGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGGCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCAt                                                                                              <  2:489894/140‑1 (MQ=255)                                       tttGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCAt                                                                                              <  2:150156/140‑1 (MQ=255)                                       tttGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCAt                                                                                              <  2:556597/140‑1 (MQ=255)                                       tttGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCAt                                                                                              <  1:1275572/140‑1 (MQ=255)                                           gTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGTGGTGTAGGCGCTGCTGGTAATaaaa                                                                                                           >  2:1412915/1‑123 (MQ=255)                                           gTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATaaaa                                                                                                           <  1:1412915/123‑1 (MQ=255)                                           gTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtac                                                                                                    <  1:485748/130‑1 (MQ=255)                                           gTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTtac                                                                                                    >  2:485748/1‑130 (MQ=255)                                           gTCACTTTGATGCCCGGTTTGATCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAg                                                                                          >  2:1078835/1‑140 (MQ=255)                                                     tGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTa                                                                                <  1:12115/140‑1 (MQ=255)                                                       cccGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTAcc                                                                              <  2:70980/140‑1 (MQ=255)                                                          ggTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCAtt                                                                           >  1:522106/1‑140 (MQ=255)                                                            tttGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTc                                                                         >  2:671919/1‑140 (MQ=255)                                                                cTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACg                                                                     >  2:225182/1‑140 (MQ=255)                                                                     atCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGtat                                                                >  2:780685/1‑140 (MQ=255)                                                                     atCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGtat                                                                >  2:131530/1‑140 (MQ=255)                                                                      tCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGtata                                                               >  1:778479/1‑140 (MQ=255)                                                                          caaACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTc                                                           >  2:955541/1‑140 (MQ=255)                                                                              ccATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGttt                                                       <  1:392149/140‑1 (MQ=255)                                                                                aTTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTc                                                     >  1:922902/1‑140 (MQ=255)                                                                                aTTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTc                                                     <  1:780685/140‑1 (MQ=255)                                                                                 ttCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCa                                                    >  2:832095/1‑140 (MQ=255)                                                                                 ttCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCa                                                    >  2:996291/1‑140 (MQ=255)                                                                                 ttCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCa                                                    >  1:555107/1‑140 (MQ=255)                                                                                               gTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCg                                      >  1:365525/1‑140 (MQ=255)                                                                                                tttGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCa                                                    >  2:890840/1‑125 (MQ=255)                                                                                                tttGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCa                                                    <  1:890840/125‑1 (MQ=255)                                                                                                tttGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCgg                                     >  1:170042/1‑140 (MQ=255)                                                                                                          gaAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGcc                           <  1:955541/140‑1 (MQ=255)                                                                                                          gaAG‑CCCCGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGcc                           <  2:170042/140‑1 (MQ=255)                                                                                                               ccccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGt                       >  2:1218403/1‑140 (MQ=255)                                                                                                               ccccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGt                       <  2:777239/140‑1 (MQ=255)                                                                                                               ccccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGt                       >  1:633416/1‑140 (MQ=255)                                                                                                               ccccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGt                       >  2:1023812/1‑140 (MQ=255)                                                                                                                 ccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTc                     <  1:832095/140‑1 (MQ=255)                                                                                                                 ccGTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTc                     <  2:922902/140‑1 (MQ=255)                                                                                                                   gTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCgg                   >  2:535954/1‑140 (MQ=255)                                                                                                                   gTCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCgg                   >  2:331944/1‑140 (MQ=255)                                                                                                                    tCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGt                  >  2:514896/1‑140 (MQ=255)                                                                                                                    tCGCGCGCCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGt                  >  2:270508/1‑140 (MQ=255)                                                                                                                         cgcCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCg             <  1:535954/140‑1 (MQ=255)                                                                                                                          gcCATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGc            <  1:1078835/140‑1 (MQ=255)                                                                                                                           ccATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCt           <  1:752369/140‑1 (MQ=255)                                                                                                                            cATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTg          <  1:671919/140‑1 (MQ=255)                                                                                                                            cATCACCACGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTg          <  2:633416/140‑1 (MQ=255)                                                                                                                                accacGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTGAGtt      <  1:893376/140‑1 (MQ=255)                                                                                                                                 ccacGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTGAGTTc     <  1:270508/140‑1 (MQ=255)                                                                                                                                    cGATGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTGAGTTCcac  >  2:580511/1‑140 (MQ=255)                                                                                                                                      aTGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCt           <  2:1032226/129‑1 (MQ=255)                                                                                                                                      aTGTGCGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCt           >  1:1032226/1‑129 (MQ=255)                                                                                                                                          |                                                                                                                                      TCACAGCAGCTATCCATACGCAGACCGAGCTTTTCAAGTTTGGTCACTTTGATGCCCGGTTTGCTCATATCAACAAACCATTCGGTGTAGACAGGTTTGTCCGGAGAAG‑CCCCGTCGCGCGCCATCACCACGATGTACGGGGTGTAGGCGCTGCTGGTAATAAAACACTTACTACCATTAAGATAAATCTTACCATTTCTACGGGTATAAGTCGTTTTCAGGCTACCCACGTCGGAGCCCGCGCCCGGTTCGGTAATCGCTGAGTTCCAC  >  NC_000913/39761‑40030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑