Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	621,652	A→G	L180L (TTG→CTG)	fepD ←	ferric enterobactin ABC transporter membrane subunit FebD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	621,652	0	A	G	100.0%	218.4 / NA	62	L180L (TTG→CTG)	fepD	ferric enterobactin ABC transporter membrane subunit FebD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/32);  total (30/32)

TCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACA  >  NC_000913/621516‑621788                                                                                                                                         |                                                                                                                                         tCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTg                                                                                                                                       <  2:199129/140‑1 (MQ=255)   gCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGAt                                                                                                                                     >  2:1010131/1‑140 (MQ=255)       ccGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTa                                                                                                                                 >  2:410638/1‑140 (MQ=255)       ccGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTa                                                                                                                                 <  1:767914/140‑1 (MQ=255)       ccGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTa                                                                                                                                 <  1:444132/140‑1 (MQ=255)       ccGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTa                                                                                                                                 >  2:767914/1‑140 (MQ=255)            gCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGt                                                                                                                            <  2:310370/140‑1 (MQ=255)            gCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGt                                                                                                                            <  1:713585/140‑1 (MQ=255)            gCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGt                                                                                                                            <  2:97263/140‑1 (MQ=255)                 aaCTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGa                                                                                                                       <  2:244402/140‑1 (MQ=255)                      ttCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGt                                                                                                                            <  2:439503/130‑1 (MQ=255)                      ttCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGt                                                                                                                            >  1:439503/1‑130 (MQ=255)                      ttCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAg                                                                                                                  >  2:1483487/1‑140 (MQ=255)                      ttCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAg                                                                                                                  >  1:670049/1‑140 (MQ=255)                                       tAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTgg                                                                                                 <  2:1060526/140‑1 (MQ=255)                                        aaTAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGt                                                                                                <  2:887764/140‑1 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCg                                                                                                     <  2:693352/131‑1 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCg                                                                                                     >  1:693352/1‑131 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt                                                                                            <  1:1010131/140‑1 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt                                                                                            <  1:1392136/140‑1 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt                                                                                            <  2:476701/140‑1 (MQ=255)                                            gcgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGt                                                                                            >  1:1249955/1‑140 (MQ=255)                                             cgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTc                                                                                           >  2:972386/1‑140 (MQ=255)                                             cgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTc                                                                                           >  1:532247/1‑140 (MQ=255)                                             cgcAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTc                                                                                           >  2:272423/1‑140 (MQ=255)                                                 gTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTc                                                                                       >  2:193851/1‑140 (MQ=255)                                                 gTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTc                                                                                       >  2:575954/1‑140 (MQ=255)                                                                  cACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAgcg                                                                      >  1:165399/1‑140 (MQ=255)                                                                    ccGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACcgccgc                                                                           <  1:925187/133‑1 (MQ=255)                                                                    ccGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACcgccgc                                                                           >  2:925187/1‑133 (MQ=255)                                                                       ggATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg                                                                 >  2:410322/1‑140 (MQ=255)                                                                       ggATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg                                                                 >  2:802007/1‑140 (MQ=255)                                                                            aGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGTGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgcccgc                                                            <  1:802007/140‑1 (MQ=255)                                                                            aGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgcccgc                                                            >  2:344103/1‑140 (MQ=255)                                                                            aGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcgcccgc                                                            >  1:259039/1‑140 (MQ=255)                                                                            aGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg                                                                 >  1:753184/1‑135 (MQ=255)                                                                            aGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCAcg                                                                 <  2:753184/135‑1 (MQ=255)                                                                                 cacTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAggg                                                       >  2:1228941/1‑140 (MQ=255)                                                                                      ttAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTaaa                                                  <  1:908798/140‑1 (MQ=255)                                                                                          ggTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCa                                              <  2:567038/140‑1 (MQ=255)                                                                                          ggTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCa                                              <  1:972386/140‑1 (MQ=255)                                                                                           gTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCAc                                             <  1:1232688/140‑1 (MQ=255)                                                                                             aTGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGTGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCg                                           <  1:581550/140‑1 (MQ=255)                                                                                              tGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCgg                                          >  2:113468/1‑140 (MQ=255)                                                                                              tGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCgg                                          >  2:1175546/1‑140 (MQ=255)                                                                                              tGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTAAAACGCACCgg                                          >  2:1162872/1‑140 (MQ=255)                                                                                               gTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGa                                         <  2:670049/140‑1 (MQ=255)                                                                                                    ttGCGAATGTCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTTGACGCCGGGATTAAGCAGCGCGATGCCGCGGGGAAGTCCTTCCAGCACCGCCGCCACCGCCACGCCTGCCAGGGTTAATCTTACCCGACTTaa                                    >  2:1058960/1‑140 (MQ=255)                                                                                                      gCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACt                                  <  2:165399/140‑1 (MQ=255)                                                                                                       cGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTaaa                                                  >  2:329544/1‑123 (MQ=255)                                                                                                       cGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTaaa                                                  <  1:329544/123‑1 (MQ=255)                                                                                                       cGAATATCCAGCGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTg                                 >  2:350384/1‑140 (MQ=255)                                                                                                                  cGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCt                      <  2:259039/140‑1 (MQ=255)                                                                                                                  cGAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCt                      <  1:925870/140‑1 (MQ=255)                                                                                                                   gAACCGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTg                     <  2:532247/140‑1 (MQ=255)                                                                                                                      ccGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTGACTGCCCGCCGCCCTGACt                  <  1:1175546/140‑1 (MQ=255)                                                                                                                      ccGGCTTGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACt                  >  1:1014640/1‑140 (MQ=255)                                                                                                                            tGCCAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGt            >  1:552860/1‑140 (MQ=255)                                                                                                                            tGCCAGAAACGCAGCTGATCGCAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGt            >  2:1068982/1‑140 (MQ=255)                                                                                                                              ccAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTaa          <  1:350384/140‑1 (MQ=255)                                                                                                                              ccAGAAACGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTaa          <  1:1228941/140‑1 (MQ=255)                                                                                                                                  aaaCGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTAAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGc      <  1:1162872/140‑1 (MQ=255)                                                                                                                                     cGCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGcaac   <  1:410638/140‑1 (MQ=255)                                                                                                                                      gCAGCTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGcaaca  <  2:742754/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TCGCTGCCGAGGCTCAAACTGTTCAGCGCGCGACTCAGTAATAGCGCAGTTGCTCCGGCGATCAGCACCGGGATCAGCACCACTTTTAAGGTATGTAGATTGCGAATATCCAGCGAACCGGCTTGCCAGAAACGCAACTGATCGTAGACGTCAGGATTAAGCAGGGCGATGCCGCTGGTCAGTCCTTCCAGCACCGCCGCCAGCGCCACGCCCGCCAGGGTTAAACGCACCGGACTTAACTGCCCGCCGCCCTGACTGCCGGTAAAGGCAACA  >  NC_000913/621516‑621788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑