Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,772	A→G	F398L (TTC→CTC)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase DacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,772	0	A	G	100.0%	210.6 / NA	59	F398L (TTC→CTC)	dacA	D‑alanyl‑D‑alanine carboxypeptidase DacA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/24);  total (35/24)

TCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTT  >  NC_000913/662639‑662904                                                                                                                                      |                                                                                                                                     tCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCAttaa                                                                                                                                >  2:128922/1‑140 (MQ=255) tCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCAttaa                                                                                                                                >  2:158352/1‑140 (MQ=255)  cATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCAttaat                                                                                                                               <  2:911138/140‑1 (MQ=255)  cATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCAttaat                                                                                                                               <  1:158352/140‑1 (MQ=255)          cTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTa                                                                                                                       <  1:1116893/140‑1 (MQ=255)               gCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCaa                                                                                                                  >  2:1272448/1‑140 (MQ=255)                         aTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGcc                                                                                                        <  1:189165/140‑1 (MQ=255)                          ttAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCg                                                                                                       <  2:872023/140‑1 (MQ=255)                           tAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCga                                                                                                      <  2:1225338/140‑1 (MQ=255)                                  cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGtt                                                                                               >  2:806759/1‑140 (MQ=255)                                  cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGtt                                                                                               >  1:76053/1‑140 (MQ=255)                                          gCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg                                                                                       >  1:902944/1‑140 (MQ=255)                                               aaGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAtt                                                                                  <  1:83120/140‑1 (MQ=255)                                                     ggAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGc                                                                            <  1:966891/140‑1 (MQ=255)                                                      gAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCa                                                                           >  1:500068/1‑140 (MQ=255)                                                      gAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCa                                                                           >  2:572790/1‑140 (MQ=255)                                                            tttACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACaa                                                                     <  1:806759/140‑1 (MQ=255)                                                               aCTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCa                                                                  <  2:500068/140‑1 (MQ=255)                                                                cTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAg                                                                 <  2:1004052/140‑1 (MQ=255)                                                                   aTGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCgg                                                              >  2:656836/1‑140 (MQ=255)                                                                    tGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCggg                                                             <  2:902944/140‑1 (MQ=255)                                                                           tataTGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCt                                                      >  2:1025789/1‑140 (MQ=255)                                                                              atGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGa                                                   >  1:608117/1‑140 (MQ=255)                                                                               tGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGAt                                                  >  2:1121988/1‑140 (MQ=255)                                                                                ggggACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAg                                                                 <  1:169694/124‑1 (MQ=255)                                                                                ggggACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAg                                                                 >  2:169694/1‑124 (MQ=255)                                                                                  ggACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGt                                               >  1:1389989/1‑140 (MQ=255)                                                                                   gACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCggg                                                             <  2:1436693/125‑1 (MQ=255)                                                                                   gACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCggg                                                             >  1:1436693/1‑125 (MQ=255)                                                                                   gACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtt                                              >  2:1296597/1‑140 (MQ=255)                                                                                   gACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtt                                              >  1:1070387/1‑140 (MQ=255)                                                                                   gACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtt                                              >  1:336586/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  2:908024/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  2:263707/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:912536/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:1038028/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:227369/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:318733/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:788516/1‑140 (MQ=255)                                                                                     cGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGtttt                                            >  1:643650/1‑140 (MQ=255)                                                                                          aTTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCAt                                       <  1:1025789/140‑1 (MQ=255)                                                                                              cacTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGA‑TTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGc                                  >  2:303485/1‑140 (MQ=255)                                                                                               acTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGc                                  >  2:1473643/1‑140 (MQ=255)                                                                                                 tttCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTg                                >  2:1469159/1‑140 (MQ=255)                                                                                                  ttCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTgg                               <  1:572790/140‑1 (MQ=255)                                                                                                  ttCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTgg                               <  2:761404/140‑1 (MQ=255)                                                                                                  ttCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTgg                               >  2:204718/1‑140 (MQ=255)                                                                                                      aGTGTTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGt                           >  2:1002924/1‑140 (MQ=255)                                                                                                         gtTTAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGa                        <  1:1469159/140‑1 (MQ=255)                                                                                                           ttAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATa                      >  1:893716/1‑140 (MQ=255)                                                                                                            tAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAg                     <  1:656836/140‑1 (MQ=255)                                                                                                            tAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAg                     <  1:908024/140‑1 (MQ=255)                                                                                                            tAATTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAg                     <  1:128922/140‑1 (MQ=255)                                                                                                             aaTTTTTAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGt                    >  2:268926/1‑140 (MQ=255)                                                                                                                  ttAACCAAACCAGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCcga               <  1:268926/140‑1 (MQ=255)                                                                                                                    aaccaaaccaGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCcgacg             >  2:1335139/1‑140 (MQ=255)                                                                                                                      ccaaaccaGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCgacgac           <  1:488778/140‑1 (MQ=255)                                                                                                                          accaGTGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCACCAAGCTGGAAGTTGATAGTTCCGACGACCTGa       <  2:1070387/140‑1 (MQ=255)                                                                                                                               tGATGGAGCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCtt  >  1:1485863/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     TCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTT  >  NC_000913/662639‑662904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑