Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,867	C→T	D26D (GAC→GAT)	chiQ →	lipoprotein ChiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,867	0	C	T	97.7%	300.1 / ‑3.6	87	D26D (GAC→GAT)	chiQ	lipoprotein ChiQ
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (35/50);  total (36/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGA  >  NC_000913/709730‑709998                                                                                                                                          |                                                                                                                                      ccATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAg                                                                                                                                     <  2:217447/140‑1 (MQ=255)  cATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGACATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAgt                                                                                                                                    >  2:1330504/1‑140 (MQ=255)       tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCt                                                                                                                               >  2:667712/1‑140 (MQ=255)       tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCt                                                                                                                               >  1:738443/1‑140 (MQ=255)       tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCt                                                                                                                               >  1:206882/1‑140 (MQ=255)       tctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCt                                                                                                                               >  2:1196252/1‑140 (MQ=255)        ctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTg                                                                                                                              >  1:1109748/1‑140 (MQ=255)        ctGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTg                                                                                                                              >  2:1011460/1‑140 (MQ=255)         tGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  2:1335461/1‑140 (MQ=255)         tGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  2:1504996/140‑1 (MQ=255)         tGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  1:786080/140‑1 (MQ=255)            tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  1:1235735/137‑1 (MQ=255)            tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  2:1235735/1‑137 (MQ=255)            tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  2:158/1‑137 (MQ=255)            tGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  1:158/137‑1 (MQ=255)              cccGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAagag                                                                                                                        >  1:606010/1‑140 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  2:907356/133‑1 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  1:907356/1‑133 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  2:1037925/1‑133 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  1:1037925/133‑1 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGc                                                                                                                      >  1:1454411/1‑140 (MQ=255)                cGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGc                                                                                                                      >  1:151880/1‑140 (MQ=255)                  aCGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             >  2:521487/1‑131 (MQ=255)                  aCGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGa                                                                                                                             <  1:521487/131‑1 (MQ=255)                   cgcgGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATa                                                                                                                   >  2:122620/1‑140 (MQ=255)                    gcgGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATAc                                                                                                                  <  1:1011460/140‑1 (MQ=255)                        cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAgcg                                                                                                              <  1:441396/140‑1 (MQ=255)                        cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAgcg                                                                                                              <  1:570950/140‑1 (MQ=255)                        cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAgcg                                                                                                              <  2:1224901/140‑1 (MQ=255)                        cAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAgcg                                                                                                              <  1:699134/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:284817/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:658076/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  1:1411493/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:709352/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  1:1196252/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:738443/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:135642/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  1:1193174/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:504405/140‑1 (MQ=255)                          ggTTTTGTGCCTGCCACACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGcc                                                                                                            <  2:1170162/140‑1 (MQ=255)                             tttGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGt                                                                                                         <  1:558043/140‑1 (MQ=255)                             tttGCGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGGTTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGCGCGCAATCAACCGCGCCCCAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGt                                                                                                         >  2:1473977/1‑140 (MQ=255)                               tgtgCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTAt                                                                                                       <  2:606010/140‑1 (MQ=255)                                gtgCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATc                                                                                                      <  2:1454411/140‑1 (MQ=255)                                  gcctgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCa‑‑a                                                                                                  <  1:703349/140‑1 (MQ=255)                                     tgccGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑acac                                                                                               >  1:1258259/1‑140 (MQ=255)                                               ttGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGt                                                                                     >  1:1032929/1‑140 (MQ=255)                                                  aGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCg                                                                                  >  1:721317/1‑140 (MQ=255)                                                       tttGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGa                                                                             >  1:1029787/1‑140 (MQ=255)                                                       tttGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGa                                                                             <  1:239926/140‑1 (MQ=255)                                                              tGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAAtt                                                                      >  1:1358629/1‑140 (MQ=255)                                                               gAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTg                                                                     >  1:131077/1‑140 (MQ=255)                                                               gAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTg                                                                     >  1:820868/1‑140 (MQ=255)                                                                       tGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGc                                                             <  1:1330504/140‑1 (MQ=255)                                                                          ttCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCTCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCag                                                          >  1:64090/1‑140 (MQ=255)                                                                           tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                         <  1:1335461/140‑1 (MQ=255)                                                                           tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                         <  2:174347/140‑1 (MQ=255)                                                                           tctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCaga                                                         <  2:1258259/140‑1 (MQ=255)                                                                            ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  2:344207/140‑1 (MQ=255)                                                                            ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  2:100744/140‑1 (MQ=255)                                                                            ctcATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  1:1081890/140‑1 (MQ=255)                                                                              cATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCagag                                                        <  1:1244491/140‑1 (MQ=255)                                                                                tCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCgtg                                                    <  2:1032929/140‑1 (MQ=255)                                                                                       aaTGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACg                                             >  1:275629/1‑140 (MQ=255)                                                                                         tGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTg                                           <  2:190644/140‑1 (MQ=255)                                                                                          ggCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGc                                          <  1:667712/140‑1 (MQ=255)                                                                                           gCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCt                                         >  2:214181/1‑140 (MQ=255)                                                                                            cATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTg                                        <  2:151880/140‑1 (MQ=255)                                                                                            cATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTg                                        >  1:1193801/1‑140 (MQ=255)                                                                                            cATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTg                                        <  2:1029787/140‑1 (MQ=255)                                                                                              tCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaa                                      >  2:1204831/1‑140 (MQ=255)                                                                                                 gggCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTgaagaa                                   >  2:790941/1‑140 (MQ=255)                                                                                                    cTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGaagaaaga                                <  1:703660/140‑1 (MQ=255)                                                                                                     tGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAagag                               >  1:950933/1‑140 (MQ=255)                                                                                                      ggTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAgaga                              >  2:1474360/1‑140 (MQ=255)                                                                                                       gTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGaa                             <  2:206882/140‑1 (MQ=255)                                                                                                       gTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGaa                             <  2:721317/140‑1 (MQ=255)                                                                                                         aGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACCCCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAaac                           <  1:214181/140‑1 (MQ=255)                                                                                                           cTTGTGCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAaacaa                         >  1:358261/1‑140 (MQ=255)                                                                                                              gtgCGCAATCAACCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACAt                      <  2:529257/140‑1 (MQ=255)                                                                                                                        aaCCGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTg            <  2:358261/140‑1 (MQ=255)                                                                                                                          ccGCGCCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGcc          <  2:1193801/140‑1 (MQ=255)                                                                                                                              gcCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGAcc      >  1:102347/1‑140 (MQ=255)                                                                                                                              gcCACAGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGAcc      >  2:1402444/1‑140 (MQ=255)                                                                                                                                 acaGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAgg   <  2:820868/140‑1 (MQ=255)                                                                                                                                  caGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGa  >  2:384062/1‑140 (MQ=255)                                                                                                                                  caGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGa  <  2:363676/140‑1 (MQ=255)                                                                                                                                  caGGAAGATAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGa  <  2:993311/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CCATCTTCTGATGCCCGACGCGGCAGGTTTTGTGCCTGCCGCACGTTTGAGGAATTTGCTATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCAACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCA‑‑ACACCGCACAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAGAAACAACATCAGCAGTTTGCCGACCAGGA  >  NC_000913/709730‑709998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑