Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	763,438	A→G	H142R (CAC→CGC)	sucC →	succinyl‑CoA synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	763,438	0	A	G	100.0%	185.1 / NA	56	H142R (CAC→CGC)	sucC	succinyl‑CoA synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/36);  total (20/36)

TCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCA  >  NC_000913/763301‑763572                                                                                                                                          |                                                                                                                                       tCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGcc                                                                                                                                      <  1:947985/140‑1 (MQ=255)   tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTg                                                                                                                                    <  1:193753/140‑1 (MQ=255)   tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTg                                                                                                                                    <  1:839680/140‑1 (MQ=255)   tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTg                                                                                                                                    <  2:1296958/140‑1 (MQ=255)   tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTg                                                                                                                                    <  2:213067/140‑1 (MQ=255)   tGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTg                                                                                                                                    <  1:716126/140‑1 (MQ=255)     gTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGAt                                                                                                                                  >  1:443876/1‑140 (MQ=255)          agcagcGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATa                                                                                                                             >  2:635016/1‑140 (MQ=255)          agcagcGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTAGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATa                                                                                                                             >  2:1356243/1‑140 (MQ=255)               cGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGtt                                                                                                                        <  2:490016/140‑1 (MQ=255)               cGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGtt                                                                                                                        >  1:1907/1‑140 (MQ=255)                   cGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACTGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTgcgc                                                                                                                    <  2:1037188/140‑1 (MQ=255)                     atatCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCtt                                                                                                                  <  1:426451/140‑1 (MQ=255)                     atatCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCtt                                                                                                                  <  2:877919/140‑1 (MQ=255)                      tatCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTg                                                                                                                 <  2:530922/140‑1 (MQ=255)                           cTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCg                                                                                                            >  2:848387/1‑140 (MQ=255)                           cTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCg                                                                                                            <  2:419816/140‑1 (MQ=255)                            tAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGc                                                                                                           >  2:1114087/1‑140 (MQ=255)                                gagCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGAc                                                                                                       <  2:662357/140‑1 (MQ=255)                                   cTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTgg                                                                                                    >  2:103014/1‑140 (MQ=255)                                      tATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGccc                                                                                                 <  2:458709/140‑1 (MQ=255)                                      tATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGccc                                                                                                 <  2:1096695/140‑1 (MQ=255)                                        tctcGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGa                                                                                               >  1:1417964/1‑140 (MQ=255)                                          tcGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATg                                                                                             <  2:795054/140‑1 (MQ=255)                                          tcGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATg                                                                                             <  1:848387/140‑1 (MQ=255)                                          tcGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATg                                                                                             <  1:262173/140‑1 (MQ=255)                                           cGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGc                                                                                            <  2:971086/140‑1 (MQ=255)                                             gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCg                                                                                          <  1:103014/140‑1 (MQ=255)                                             gTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCg                                                                                          >  1:491066/1‑140 (MQ=255)                                                ccGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGc                                                                                            >  1:742701/1‑135 (MQ=255)                                                ccGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGc                                                                                            <  2:742701/135‑1 (MQ=255)                                                ccGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTAt                                                                                       >  2:695905/1‑140 (MQ=255)                                                      ttgACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGa                                                                                 >  1:1169007/1‑140 (MQ=255)                                                       tgACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGAc                                                                                <  1:781094/140‑1 (MQ=255)                                                        gACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGAcg                                                                               >  1:1168817/1‑140 (MQ=255)                                                        gACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGAcg                                                                               <  2:150145/140‑1 (MQ=255)                                                            gTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAg                                                                           <  2:1417964/140‑1 (MQ=255)                                                              aGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCt                                                                         <  2:443876/140‑1 (MQ=255)                                                                      tcgtGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCa                                                                 <  2:1305537/140‑1 (MQ=255)                                                                       cgtGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCaa                                                                >  1:412870/1‑140 (MQ=255)                                                                         tgtgGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAAc                                                              <  1:429501/140‑1 (MQ=255)                                                                         tgtgGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAAc                                                              >  2:429501/1‑140 (MQ=255)                                                                                tttATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCt                                                       <  2:109284/140‑1 (MQ=255)                                                                                 ttATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTg                                                      <  2:412870/140‑1 (MQ=255)                                                                                 ttATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTg                                                      <  2:1169007/140‑1 (MQ=255)                                                                                   aTGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGa                                                    <  2:818438/140‑1 (MQ=255)                                                                                           cACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTg                                                            >  2:261971/1‑124 (MQ=255)                                                                                           cACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTg                                                            <  1:261971/124‑1 (MQ=255)                                                                                                      gcgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTcagcag                                 <  1:1356243/140‑1 (MQ=255)                                                                                                      gcgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTcagcag                                 <  1:635016/140‑1 (MQ=255)                                                                                                       cgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGt                                >  2:1208953/1‑140 (MQ=255)                                                                                                       cgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGt                                >  2:150685/1‑140 (MQ=255)                                                                                                               tCGAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCaaa                        <  2:491066/140‑1 (MQ=255)                                                                                                                 gAAAAAGTGGCGGAAGAAACTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAAt                      >  1:1144113/1‑140 (MQ=255)                                                                                                                                    cTCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGAcc   >  2:76745/1‑140 (MQ=255)                                                                                                                                     tCCGCGCCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCa  <  2:1907/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCTGGTTGAAGCAGCGACCGATATCGCTAAAGAGCTGTATCTCGGTGCCGTTGTTGACCGTAGTTCCCGTCGTGTGGTCTTTATGGCCTCCACCGAAGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCA  >  NC_000913/763301‑763572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑