Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	955,270	A→G	S455P (TCG→CCG)	ycaO ←	ribosomal protein S12 methylthiotransferase accessory factor YcaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	955,270	0	A	G	100.0%	332.6 / NA	93	S455P (TCG→CCG)	ycaO	ribosomal protein S12 methylthiotransferase accessory factor YcaO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/38);  total (55/38)

GTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTA  >  NC_000913/955135‑955404                                                                                                                                        |                                                                                                                                       gTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCg                                                                                                                                    >  2:214449/1‑140 (MQ=255)     cGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCg                                                                                                                                <  2:789520/140‑1 (MQ=255)            aaTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGAcc                                                                                                                         >  2:124748/1‑140 (MQ=255)             aTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGcc                                                                                                                              >  2:94154/1‑134 (MQ=255)             aTACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGcc                                                                                                                              <  1:94154/134‑1 (MQ=255)               aCTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCaa                                                                                                                      <  1:584392/140‑1 (MQ=255)               aCTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCaa                                                                                                                      <  1:842425/140‑1 (MQ=255)                 tgatgaGTTAAACTCCATCGTCCATTCGGTCCTGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACa                                                                                                                    >  2:1216336/1‑140 (MQ=255)                 tgatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACa                                                                                                                    <  2:762317/140‑1 (MQ=255)                 tgatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACa                                                                                                                    >  1:1364812/1‑140 (MQ=255)                 tgatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACa                                                                                                                    >  1:827667/1‑140 (MQ=255)                  gatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAg                                                                                                                   <  1:430598/140‑1 (MQ=255)                  gatgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAg                                                                                                                   >  2:1146873/1‑140 (MQ=255)                    tgaGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAgat                                                                                                                 >  1:557997/1‑138 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTGTCCGGCCCGGTCGCCAGACCCAACAGCGca                                                                                                               >  2:930278/1‑140 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTca                                                                                                               >  1:118487/1‑140 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTca                                                                                                               >  1:454310/1‑140 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTca                                                                                                               >  1:178090/1‑140 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTca                                                                                                               >  2:880845/1‑140 (MQ=255)                      aGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTca                                                                                                               >  1:134934/1‑140 (MQ=255)                        ttAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacg                                                                                                             >  1:924535/1‑140 (MQ=255)                          aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgca                                                                                                           <  2:118487/140‑1 (MQ=255)                          aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgca                                                                                                           <  2:178090/140‑1 (MQ=255)                          aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgca                                                                                                           <  1:740134/140‑1 (MQ=255)                          aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgca                                                                                                           <  1:67624/140‑1 (MQ=255)                          aaaCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgca                                                                                                           <  2:827667/140‑1 (MQ=255)                            aCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgcacg                                                                                                         <  1:1054651/140‑1 (MQ=255)                            aCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgcacg                                                                                                         <  2:454310/140‑1 (MQ=255)                            aCTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTcacgcacg                                                                                                         <  2:671349/140‑1 (MQ=255)                             cTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCacgcacgc                                                                                                        >  2:1363330/1‑140 (MQ=255)                                cATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCggg                                                                                                     >  1:25226/1‑140 (MQ=255)                                cATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCggg                                                                                                     >  1:96385/1‑140 (MQ=255)                                  tCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTa                                                                                                   >  2:458269/1‑140 (MQ=255)                                  tCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTa                                                                                                   >  2:637666/1‑140 (MQ=255)                                  tCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTa                                                                                                   >  2:1285223/1‑140 (MQ=255)                                       cATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGtc                                                                                              >  1:1508566/1‑140 (MQ=255)                                           cGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGtcatca                                                                                          <  1:1232132/140‑1 (MQ=255)                                               ccagaccagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAAc                                                                                      >  1:1101052/1‑140 (MQ=255)                                                   accagaGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACcttc                                                                                  >  2:987019/1‑140 (MQ=255)                                                        agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCAt                                                                             >  2:290066/1‑140 (MQ=255)                                                        agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCAt                                                                             >  2:340236/1‑140 (MQ=255)                                                        agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCAt                                                                             >  1:95043/1‑140 (MQ=255)                                                        agCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACC‑ACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATc                                                                            >  2:874603/1‑140 (MQ=255)                                                            tGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAg                                                                         >  1:208116/1‑140 (MQ=255)                                                            tGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGATCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAg                                                                         <  2:557997/140‑1 (MQ=255)                                                              ttCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGtt                                                                       >  1:1349932/1‑140 (MQ=255)                                                               tCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTg                                                                      >  1:520799/1‑140 (MQ=255)                                                                     tCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGAt                                                                <  1:1442231/140‑1 (MQ=255)                                                                      cGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                                               <  2:1364812/140‑1 (MQ=255)                                                                        ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCt                                                                    <  2:853735/133‑1 (MQ=255)                                                                        ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCt                                                                    >  1:853735/1‑133 (MQ=255)                                                                        ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAg                                                             >  2:1459161/1‑140 (MQ=255)                                                                        ccaccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAg                                                             >  2:623614/1‑140 (MQ=255)                                                                         caccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                                               <  1:522881/137‑1 (MQ=255)                                                                         caccaGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATg                                                               >  2:522881/1‑137 (MQ=255)                                                                            cagccagcGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTc                                                         <  2:1101052/140‑1 (MQ=255)                                                                            cagccagcGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTc                                                         <  1:1036932/140‑1 (MQ=255)                                                                                   cgccagcATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAAt                                                  >  1:118086/1‑140 (MQ=255)                                                                                         cATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATcttctt                                            >  1:233943/1‑140 (MQ=255)                                                                                              cTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTcc                                       <  2:1508566/140‑1 (MQ=255)                                                                                              cTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTcc                                       <  2:208116/140‑1 (MQ=255)                                                                                              cTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTcc                                       >  2:635155/1‑140 (MQ=255)                                                                                                    aTTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCg                                 <  2:924535/140‑1 (MQ=255)                                                                                                    aTTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCg                                 <  1:1459161/140‑1 (MQ=255)                                                                                                    aTTCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCg                                 <  1:458269/140‑1 (MQ=255)                                                                                                      tCACCGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCt                               <  2:96385/140‑1 (MQ=255)                                                                                                         ccGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGcc                            <  2:25226/140‑1 (MQ=255)                                                                                                         ccGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGcc                            <  2:28762/140‑1 (MQ=255)                                                                                                          cGATACGCAGGGTGTACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCt                           <  2:1349932/140‑1 (MQ=255)                                                                                                                      tgtACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGt                        <  1:84491/131‑1 (MQ=255)                                                                                                                      tgtACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGt                        >  2:84491/1‑131 (MQ=255)                                                                                                                       gtACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGaa              >  1:424986/1‑140 (MQ=255)                                                                                                                        tACCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAAt             >  1:55215/1‑140 (MQ=255)                                                                                                                         aCCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGaaa                 <  2:174319/135‑1 (MQ=255)                                                                                                                         aCCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGaaa                 >  1:174319/1‑135 (MQ=255)                                                                                                                         aCCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATc            >  2:50931/1‑140 (MQ=255)                                                                                                                         aCCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATc            >  1:419861/1‑140 (MQ=255)                                                                                                                         aCCAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATc            >  2:82528/1‑140 (MQ=255)                                                                                                                           cAACCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGt          <  1:1309733/140‑1 (MQ=255)                                                                                                                            aaCCGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGtt         <  1:82528/140‑1 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc       >  2:816562/1‑140 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc       >  2:1246369/1‑140 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc       <  1:314910/140‑1 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc       >  2:551311/1‑140 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTc       <  2:397852/140‑1 (MQ=255)                                                                                                                              ccGTTATCCGGCCCGGTCGACAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCTAGTTGCTCGATGAGGATCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAAACGTTTc       >  2:1052300/1‑140 (MQ=255)                                                                                                                                gTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc     >  1:1246700/1‑140 (MQ=255)                                                                                                                                gTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc     >  2:686521/1‑140 (MQ=255)                                                                                                                                gTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc     >  2:957073/1‑140 (MQ=255)                                                                                                                                gTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc     >  1:708071/1‑140 (MQ=255)                                                                                                                                gTTATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCAc     >  2:988396/1‑140 (MQ=255)                                                                                                                                  tATCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGt   >  2:1253828/1‑140 (MQ=255)                                                                                                                                   aTCCGGCCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTa  <  1:340236/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GTTCCGGACTAAATACTGATGAGTTAAACTCCATCGTCCATTCGGTCCAGACCAGAGCCTGTTCCAGATCGCCACCAGCCAGCGCCAGCATGGCTTTTAATTCACCGATACGCAGGGTGTACCAACCGTTATCCGACCCGGTCGCCAGACCCAACAGCTCACGCACGCGGGTAAAGTCATCAAAACCTTCTTCATCCAGTTGCTCGATGAGGTTCAGGTAATCTTCTTTTTCCCACTCGCTGCCTGGTAGCGAAAGAATCGTTTCACGTA  >  NC_000913/955135‑955404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑