Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,073,736	C→T	G92G (GGG→GGA)	rutA ←	pyrimidine oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,073,736	0	C	T	100.0%	179.0 / NA	52	G92G (GGG→GGA)	rutA	pyrimidine oxygenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/32);  total (20/32)

CCAGTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGT  >  NC_000913/1073599‑1073871                                                                                                                                          |                                                                                                                                        ccagTCACGAGGTTGCCGCAAAAACGCCGGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGCCGATTGCCGGAGGAAACGTTAGGGTGGCAGCAGTGGCTTAAATGTGAATGCGGGAGGCCACGGCCGCCAGtcc                                                                                                                                       <  2:255473/140‑1 (MQ=255) ccagTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGtcc                                                                                                                                       <  2:1443897/140‑1 (MQ=255)     tCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGcc                                                                                                                                   >  2:1302632/1‑140 (MQ=255)       aCGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCAt                                                                                                                                 <  2:831685/140‑1 (MQ=255)         gAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCa                                                                                                                               <  1:173177/140‑1 (MQ=255)         gAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCa                                                                                                                               <  2:1465738/140‑1 (MQ=255)                ccGCCAAACCCCCCGCCAGAGATGGAGTCGATGGTTGCGGCCAAACGGGGGACGATTGCCGGAGTTAACGTTAAGGTGGCAGCAGTGGCATAAATCTGAAGGCGCGAGGCCACGGCCGCCAGTCCCGCCATCAAGGTGaa                                                                                                                        <  2:1519851/139‑1 (MQ=255)                      aaaCGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTc                                                                                                                  <  2:1057308/140‑1 (MQ=255)                       aaCGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCa                                                                                                                 <  2:1477787/140‑1 (MQ=255)                       aaCGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCa                                                                                                                 >  2:1041189/1‑140 (MQ=255)                                     aTGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCa                                                                                                   <  1:1494160/140‑1 (MQ=255)                                          gTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGATGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACt                                                                                              >  1:310881/1‑140 (MQ=255)                                          gTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACt                                                                                              >  1:166356/1‑140 (MQ=255)                                                      ggCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTgccgc                                                                                  <  2:829116/140‑1 (MQ=255)                                                           caCGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAg                                                                             >  2:1144249/2‑140 (MQ=255)                                                              gggCGACGATTGCCGGATGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                                          <  2:310881/140‑1 (MQ=255)                                                              gggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCa                                                                          <  1:1441461/140‑1 (MQ=255)                                                               ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCa                                                                                <  1:284668/133‑1 (MQ=255)                                                               ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCa                                                                                >  2:284668/1‑133 (MQ=255)                                                               ggCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCAc                                                                         <  1:846978/140‑1 (MQ=255)                                                                 cgacgaTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGc                                                                       >  2:358901/1‑140 (MQ=255)                                                                     gaTTGCCGGAGTTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                                   <  1:270735/140‑1 (MQ=255)                                                                     gaTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                                   <  1:966752/140‑1 (MQ=255)                                                                     gaTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                                   <  2:166356/140‑1 (MQ=255)                                                                     gaTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGtt                                                                   <  2:444016/140‑1 (MQ=255)                                                                      aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAgct                                                                   <  2:431539/139‑3 (MQ=255)                                                                      aTTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAgct                                                                   >  1:431539/1‑137 (MQ=255)                                                                                gTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGAc                                                        <  2:13955/140‑1 (MQ=255)                                                                                 tAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACa                                                       >  1:161902/1‑140 (MQ=255)                                                                                    cGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGAGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAggg                                                    >  2:1346391/1‑140 (MQ=255)                                                                                      ttAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcg                                                  <  1:928564/140‑1 (MQ=255)                                                                                           gTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaatc                                             >  1:553373/1‑140 (MQ=255)                                                                                             ggCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaatcga                                           <  2:225271/140‑1 (MQ=255)                                                                                              gcagcaGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat                                              <  1:101094/136‑1 (MQ=255)                                                                                              gcagcaGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaat                                              >  2:101094/1‑136 (MQ=255)                                                                                               cagcagTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGcgaaatcgaaa                                         >  1:745209/1‑140 (MQ=255)                                                                                                     tGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAg                                   >  1:556477/1‑140 (MQ=255)                                                                                                     tGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAg                                   >  2:836445/1‑140 (MQ=255)                                                                                                       gCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTg                                 >  1:1184709/1‑140 (MQ=255)                                                                                                         gTAAATCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCt                               <  1:442351/140‑1 (MQ=255)                                                                                                              tCTGAATGCGCGAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCt                          <  2:1184709/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  2:556477/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  1:1041189/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  2:198713/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  2:161902/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  1:1302632/140‑1 (MQ=255)                                                                                                                        cgAGGTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACaa                <  1:1144249/140‑1 (MQ=255)                                                                                                                            gTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGc            >  1:559902/1‑140 (MQ=255)                                                                                                                            gTCACGGCCGCCAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGc            <  1:1346391/140‑1 (MQ=255)                                                                                                                                     gccAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAg   <  2:745209/140‑1 (MQ=255)                                                                                                                                      ccAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCt           <  1:849183/131‑1 (MQ=255)                                                                                                                                      ccAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCt           >  2:849183/1‑131 (MQ=255)                                                                                                                                      ccAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGt  >  1:913990/1‑140 (MQ=255)                                                                                                                                      ccAGTCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGt  >  1:703192/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CCAGTCACGAGGTTGACGCCAAAACGCCCGCCAGAGATGGAGTCGATGGTTGCGGCCATACGGGCGACGATTGCCGGAGGTAACGTTAAGGTGGCAGCAGTGGCGTAAATCTGAATGCGCGAGGTCACGGCCGCCAGCCCCGCCATCAAGGTGAACGACTCAAGGTTGTGATCCCAGAACTCAGTTTTGCCGCCAAAGCCACGCAGTTTGATCATCGACAGGGCGAAATCGAAATGGTAGTGCTCCGCTTTTTGCACAATGGCTTTATTCAGT  >  NC_000913/1073599‑1073871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑