Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,122,081	A→G	F176S (TTT→TCT)	pyrC ←	dihydroorotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,122,081	0	A	G	100.0%	269.4 / NA	75	F176S (TTT→TCT)	pyrC	dihydroorotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/32);  total (43/32)

TACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTT  >  NC_000913/1121946‑1122216                                                                                                                                        |                                                                                                                                        tACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAAc                                                                                                                                     >  2:424411/1‑140 (MQ=255)   cAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGa                                                                                                                                   >  2:1406560/1‑140 (MQ=255)    aGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGAc                                                                                                                                  >  1:1337142/1‑140 (MQ=255)      gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACtt                                                                                                                                >  1:1336312/1‑140 (MQ=255)      gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACtt                                                                                                                                >  2:102262/1‑140 (MQ=255)      gTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACtt                                                                                                                                >  1:1492182/1‑140 (MQ=255)         cGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCa                                                                                                                             >  1:258995/1‑140 (MQ=255)           gACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAgc                                                                                                                           >  1:833667/1‑140 (MQ=255)                 cgcCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTc                                                                                                                     >  2:1227969/1‑140 (MQ=255)                    cTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAgg                                                                                                                  >  2:325812/1‑140 (MQ=255)                      ccAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGgcg                                                                                                                <  2:1492182/140‑1 (MQ=255)                       cAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGgcgc                                                                                                               <  2:50770/140‑1 (MQ=255)                        aaCCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCt                                                                                                              <  1:1227969/140‑1 (MQ=255)                           cAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGgc                                                                                                           <  2:1336312/140‑1 (MQ=255)                              cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGgcgc                                                                                                         <  2:540846/139‑1 (MQ=255)                              cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGgcgc                                                                                                         >  1:540846/1‑139 (MQ=255)                              cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCa                                                                                                        >  1:381906/1‑140 (MQ=255)                              cATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCa                                                                                                        >  2:146618/1‑140 (MQ=255)                                tatGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCaga                                                                                                      >  1:848718/1‑140 (MQ=255)                                 atGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCagag                                                                                                     >  2:809510/1‑140 (MQ=255)                                  tGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAgg                                                                                                    <  2:833667/140‑1 (MQ=255)                                   ggTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGt                                                                                                   >  1:1024216/1‑140 (MQ=255)                                   ggTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGt                                                                                                   >  1:1113604/1‑140 (MQ=255)                                        cGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCAt                                                                                              >  2:1128797/1‑140 (MQ=255)                                        cGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCAt                                                                                              >  1:538445/1‑140 (MQ=255)                                         ggTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATc                                                                                             <  2:381906/140‑1 (MQ=255)                                           ttAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCAc                                                                                           <  2:1024216/140‑1 (MQ=255)                                             aaaCATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGc                                                                                         <  2:227355/140‑1 (MQ=255)                                                        gctgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAg                                                                              >  2:491519/1‑140 (MQ=255)                                                        gctgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAg                                                                              >  2:889371/1‑140 (MQ=255)                                                         ctgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgc                                                                             >  1:518585/1‑140 (MQ=255)                                                         ctgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTCCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgc                                                                             <  2:1333621/140‑1 (MQ=255)                                                          tgcGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgcg                                                                            >  1:1254930/1‑140 (MQ=255)                                                             ggAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAgcgcgc                                                                         >  1:1427734/1‑140 (MQ=255)                                                                    tggtggCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGa                                                                  >  2:286704/1‑140 (MQ=255)                                                                         gCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCaaa                                                             >  1:1007172/1‑140 (MQ=255)                                                                         gCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCaaa                                                             >  1:1032249/1‑140 (MQ=255)                                                                              cagccGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGt                                                        <  1:889371/140‑1 (MQ=255)                                                                               agccGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTc                                                       <  2:518585/140‑1 (MQ=255)                                                                                gccGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCg                                                      >  2:330139/1‑140 (MQ=255)                                                                                  cGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGat                                                    <  1:491519/140‑1 (MQ=255)                                                                                      cATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCt                                                <  2:1427734/140‑1 (MQ=255)                                                                                        tttCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGc                                              <  2:1254930/140‑1 (MQ=255)                                                                                         ttCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCa                                             <  1:809510/140‑1 (MQ=255)                                                                                          tCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAt                                            <  1:325812/140‑1 (MQ=255)                                                                                           ccGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAtg                                           >  2:112697/1‑140 (MQ=255)                                                                                           ccGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAtg                                           <  2:258995/140‑1 (MQ=255)                                                                                             gTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCAtgtg                                         <  2:538445/140‑1 (MQ=255)                                                                                             gTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGCCGATATCTGCAtgtg                                         <  2:799232/140‑1 (MQ=255)                                                                                              tCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATgtgt                                        <  1:112697/140‑1 (MQ=255)                                                                                              tCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATgtgt                                        >  2:38498/1‑140 (MQ=255)                                                                                               cACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTc                                       >  2:133991/1‑140 (MQ=255)                                                                                                 cGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCAc                                     >  1:539152/1‑140 (MQ=255)                                                                                                  ggACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACt                                    <  2:280604/140‑1 (MQ=255)                                                                                                   gACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACtt                                   <  2:1337142/140‑1 (MQ=255)                                                                                                   gACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACtt                                   >  2:387143/1‑140 (MQ=255)                                                                                                      aTAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCAc                                <  2:1007172/140‑1 (MQ=255)                                                                                                        aGTCGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCa                              <  2:921706/140‑1 (MQ=255)                                                                                                          tCGGCAGCATCTTTGGCGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATg                            >  1:165353/1‑140 (MQ=255)                                                                                                           cGGCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGc                           <  1:133991/140‑1 (MQ=255)                                                                                                            ggCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCa                          >  2:930143/1‑140 (MQ=255)                                                                                                            ggCAGCATCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCa                          <  1:930143/140‑1 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCa                                      >  2:667469/1‑126 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCa                                      <  1:667469/126‑1 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCa                          >  1:34708/1‑138 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCa                          <  2:34708/138‑1 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCAcc                        >  2:838271/1‑140 (MQ=255)                                                                                                              cagcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCAcc                        >  1:1121232/1‑140 (MQ=255)                                                                                                                gcaTCTTTGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAg                      <  2:539152/140‑1 (MQ=255)                                                                                                                     tttGGTGGTGATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCg                 <  2:752446/140‑1 (MQ=255)                                                                                                                            gtgATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCGCCATGCACCAGt                     <  2:610284/129‑1 (MQ=255)                                                                                                                            gtgATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGt                     >  1:610284/1‑129 (MQ=255)                                                                                                                             tgATGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCg         >  2:242811/1‑140 (MQ=255)                                                                                                                               aTGTGCTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGAt       >  1:414684/1‑140 (MQ=255)                                                                                                                                    cTCAGAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGAtttttt  >  2:1485251/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTGGTGATGTGCTCAAAAACGACTTTCAGCGCAGTCAGGCGCTGGCGCAGAGGTTCCATCACGCTTTCTATAAAGCGCGCTTCACGATCAAAAATGTCGATATCTGCATGTGTCACTTCACCATGCACCAGTAGCGGCATACCGATTTTTT  >  NC_000913/1121946‑1122216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑