Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,992	A→G	R157R (CGT→CGC)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,992	0	A	G	100.0%	247.9 / NA	70	R157R (CGT→CGC)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/40);  total (30/40)

GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTT  >  NC_000913/177856‑178122                                                                                                                                         |                                                                                                                                   gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAGTTGCAGAAAAACGCGt                                                                                                                                 >  1:1477875/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                 >  2:1114790/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                 >  1:1473083/1‑140 (MQ=255)     gcgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGttttt                                                                                                                             >  1:145771/1‑140 (MQ=255)       ggCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTa                                                                                                                           >  2:531787/1‑140 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCTCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                         <  1:973404/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                         <  1:556569/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                         <  2:870241/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                         <  1:1346233/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                         <  2:300195/140‑1 (MQ=255)          tAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGt                                                                                                                        <  1:531787/140‑1 (MQ=255)              ttGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTAt                                                                                                                    <  1:1206443/140‑1 (MQ=255)               tGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATc                                                                                                                   <  1:204923/140‑1 (MQ=255)                gCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCa                                                                                                                  <  1:804029/140‑1 (MQ=255)                     cAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCata                                                                                                             <  2:507628/140‑1 (MQ=255)                       gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                           <  1:937931/140‑1 (MQ=255)                         gAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTgc                                                                                                         <  2:1209982/140‑1 (MQ=255)                              cGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCttt                                                                                                    >  2:1450741/1‑140 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAGTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                            <  2:1477875/140‑1 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                            >  2:1006620/1‑140 (MQ=255)                                        tAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGcgcg                                                                                          <  2:1000693/140‑1 (MQ=255)                                         aaaGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                                                                                         <  2:1138251/140‑1 (MQ=255)                                         aaaGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                                                                                         >  2:90590/1‑140 (MQ=255)                                           aGATTTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTAc                                                                                       <  2:818998/140‑1 (MQ=255)                                           aGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTAc                                                                                       <  2:1278827/140‑1 (MQ=255)                                           aGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTAc                                                                                       <  1:1006620/140‑1 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCa                                                                                                  <  2:263869/126‑1 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCa                                                                                                  >  1:263869/1‑126 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                    >  2:389177/1‑140 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                    >  1:671380/1‑140 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                    >  1:281425/1‑140 (MQ=255)                                               gTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGcgcg                                                                                          <  1:872055/133‑1 (MQ=255)                                               gTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGcgcg                                                                                          >  2:872055/1‑133 (MQ=255)                                               gTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACACTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGTGCGTACTGAt                                                                                   >  1:212264/1‑140 (MQ=255)                                                ttttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTg                                                                                     <  2:693056/137‑1 (MQ=255)                                                ttttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTg                                                                                     >  1:693056/1‑137 (MQ=255)                                                  ttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                                                                                         >  2:1460645/1‑131 (MQ=255)                                                  ttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGt                                                                                         <  1:1460645/131‑1 (MQ=255)                                                    ctcCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCcagc                                                                              <  1:935859/140‑1 (MQ=255)                                                       cGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAgg                                                                           <  1:1114790/140‑1 (MQ=255)                                                                cTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGcgcg                                                                  <  1:90590/140‑1 (MQ=255)                                                                  tCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGc                                                                <  2:145771/140‑1 (MQ=255)                                                                       tACCTGGTTCTGAATCGACACTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGTGCGTACTGATCCAGCAGGGATTGCGCGGCTTGtt                                                           <  2:212264/140‑1 (MQ=255)                                                                         ccTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCg                                                         >  2:241144/1‑140 (MQ=255)                                                                            ggTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGcc                                                      <  2:1167359/140‑1 (MQ=255)                                                                            ggTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGcc                                                      <  1:1000114/140‑1 (MQ=255)                                                                            ggTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTCATCCAGCAGGGATTGCGCGGCTTGTTCGGcc                                                      >  1:396608/1‑140 (MQ=255)                                                                                 tGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTCATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTc                                                 <  2:396608/140‑1 (MQ=255)                                                                                   aaTCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTg                                               >  2:1106229/1‑140 (MQ=255)                                                                                           ctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGa                                       <  1:241144/140‑1 (MQ=255)                                                                                           ctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGa                                       <  2:671380/140‑1 (MQ=255)                                                                                            ttttCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGAc                                      <  2:1117973/140‑1 (MQ=255)                                                                                                 cACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAg                                 <  1:1417640/140‑1 (MQ=255)                                                                                                   cTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                           <  1:874583/64‑1 (MQ=255)                                                                                                   cTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                           >  2:874583/1‑64 (MQ=255)                                                                                                      gTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGc                            >  2:1181988/1‑140 (MQ=255)                                                                                                      gTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGc                            >  1:1342183/1‑140 (MQ=255)                                                                                                           tGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGtt                       >  1:980907/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                  >  1:544556/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                  >  2:1440219/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                  >  2:438381/1‑140 (MQ=255)                                                                                                                  ttAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTaa                <  2:380410/140‑1 (MQ=255)                                                                                                                         cGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGATTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTg         <  2:1342183/140‑1 (MQ=255)                                                                                                                           aaTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGtt                       <  2:523118/124‑1 (MQ=255)                                                                                                                           aaTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGtt                       >  1:523118/1‑124 (MQ=255)                                                                                                                           aaTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGc       >  1:1441803/1‑140 (MQ=255)                                                                                                                           aaTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGc       >  2:958420/1‑140 (MQ=255)                                                                                                                              tGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAt    <  1:1181988/140‑1 (MQ=255)                                                                                                                                cAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAttt  >  2:395542/1‑140 (MQ=255)                                                                                                                                cAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAttt  <  1:1450741/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTT  >  NC_000913/177856‑178122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑