Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,409,337	T→C	P10P (CCT→CCC)	ynaL →	protein YnaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,409,337	0	T	C	100.0%	225.1 / NA	63	P10P (CCT→CCC)	ynaL	protein YnaL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/29);  total (34/29)

AGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAG  >  NC_000913/1409202‑1409456                                                                                                                                        |                                                                                                                        aGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTcc                                                                                                                     <  1:787054/140‑1 (MQ=255)   cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCt                                                                                                                   >  2:723410/1‑140 (MQ=255)   cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCt                                                                                                                   >  1:992757/1‑140 (MQ=255)   cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCt                                                                                                                   >  1:828454/1‑140 (MQ=255)   cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCt                                                                                                                   >  1:766097/1‑140 (MQ=255)   cacaATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCt                                                                                                                   >  1:492549/1‑140 (MQ=255)       aTTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAAc                                                                                                               <  1:1338883/140‑1 (MQ=255)            ttCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGAtt                                                                                                          <  1:671512/140‑1 (MQ=255)             tCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTc                                                                                                         >  1:959407/1‑140 (MQ=255)             tCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTc                                                                                                         >  1:279375/1‑140 (MQ=255)                    acgCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGa                                                                                                  <  2:428294/140‑1 (MQ=255)                      gCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATc                                                                                                <  2:492549/140‑1 (MQ=255)                      gCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATc                                                                                                <  2:959407/140‑1 (MQ=255)                             ttGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTcc                                                                                         <  1:723410/140‑1 (MQ=255)                              tgatgaGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCa                                                                                        <  2:766097/140‑1 (MQ=255)                                atgaGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGt                                                                                      <  1:1133465/140‑1 (MQ=255)                                      cGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGGAGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCccga                                                                                >  2:1354940/1‑140 (MQ=255)                                      cGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCccga                                                                                >  1:260409/1‑140 (MQ=255)                                             tttttGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCcgatccgatc                                                                         >  1:1286058/1‑140 (MQ=255)                                                ttGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCt                                                                      <  1:1354940/140‑1 (MQ=255)                                                   ccTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGc                                                                   >  2:1054171/1‑140 (MQ=255)                                                   ccTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGc                                                                   >  2:21362/1‑140 (MQ=255)                                                   ccTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGc                                                                   >  1:744433/1‑140 (MQ=255)                                                    cTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGcc                                                                  <  2:260409/140‑1 (MQ=255)                                                    cTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGACCCGACCCCTCGcc                                                                  >  2:795703/1‑140 (MQ=255)                                                      gTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCg                                                                <  2:828454/140‑1 (MQ=255)                                                      gTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCg                                                                <  2:1286058/140‑1 (MQ=255)                                                      gTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCg                                                                >  2:635221/1‑140 (MQ=255)                                                      gTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCg                                                                <  2:19406/140‑1 (MQ=255)                                                      gTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCg                                                                <  2:1458100/140‑1 (MQ=255)                                                               cAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATg                                                       >  2:485938/1‑140 (MQ=255)                                                                 gCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGcc                                                     >  2:55364/1‑140 (MQ=255)                                                                  cGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCt                                                    >  2:1444411/1‑140 (MQ=255)                                                                  cGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCt                                                    >  1:1423898/1‑140 (MQ=255)                                                                  cGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCt                                                    >  1:373693/1‑140 (MQ=255)                                                                  cGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCt                                                    >  1:751397/1‑140 (MQ=255)                                                                        cccTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACcca                                              >  2:465405/1‑140 (MQ=255)                                                                         ccTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccac                                             <  2:373693/140‑1 (MQ=255)                                                                          cTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccacc                                            <  1:598997/140‑1 (MQ=255)                                                                             tAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCaccacc                                         <  2:751397/140‑1 (MQ=255)                                                                             tAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCaccacc                                         <  1:635221/140‑1 (MQ=255)                                                                             tAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCaccacc                                         <  2:1423898/140‑1 (MQ=255)                                                                                  gTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccacc                                            <  1:623383/132‑1 (MQ=255)                                                                                  gTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccacc                                            >  2:623383/1‑132 (MQ=255)                                                                                   tCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATga                                   >  1:1480713/1‑140 (MQ=255)                                                                                   tCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATga                                   >  1:647016/1‑140 (MQ=255)                                                                                     cACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccacc                                            <  2:1212273/129‑1 (MQ=255)                                                                                     cACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACccacc                                            >  1:1212273/1‑129 (MQ=255)                                                                                     cACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCaccacc                                         <  1:50034/132‑1 (MQ=255)                                                                                     cACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCaccacc                                         >  2:50034/1‑132 (MQ=255)                                                                                      aCCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATgaaga                                <  2:1480713/140‑1 (MQ=255)                                                                                             gACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGAtt                         >  2:861764/1‑140 (MQ=255)                                                                                                  gagGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAAtt                    >  1:290252/1‑140 (MQ=255)                                                                                                  gagGGAAATATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAAtt                    >  1:792619/1‑140 (MQ=255)                                                                                                       aaaTATGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGc               <  1:861764/140‑1 (MQ=255)                                                                                                          tatGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATc            >  1:578401/1‑140 (MQ=255)                                                                                                            tGACGACACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGt          <  1:1444411/140‑1 (MQ=255)                                                                                                             gacgacACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGt                  <  1:1173758/131‑1 (MQ=255)                                                                                                             gacgacACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGt                  >  2:1173758/1‑131 (MQ=255)                                                                                                             gacgacACTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTg         >  2:1394697/1‑140 (MQ=255)                                                                                                                 acacTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCg     <  1:997618/140‑1 (MQ=255)                                                                                                                  cacTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGt    <  2:647016/140‑1 (MQ=255)                                                                                                                    cTTATTTATTTGCAAATTCCCGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAg  >  2:440327/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                        AGCACAATTGATTCGTACGACGCCGACTTTGATGAGTCGGCTTTTTTTTGCCTGTTATTTATCAGCGTCTACCCTTTAAGAGTCCACCCAATGACCAGAGGGAAATATGACGACACTTATTTATTTGCAAATTCCTGTCCCTGAACCGATTCCTGGCGATCCTGTTCCAGTGCCCGATCCGATCCCTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAG  >  NC_000913/1409202‑1409456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑