Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,270	A→G	I1049V (ATC→GTC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,270	0	A	G	100.0%	321.6 / NA	90	I1049V (ATC→GTC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/44);  total (46/44)

CGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCAAA  >  NC_000913/208133‑208402                                                                                                                                          |                                                                                                                                     cgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTc                                                                                                                                    >  1:1150403/1‑140 (MQ=255)          tGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGa                                                                                                                           <  2:613875/140‑1 (MQ=255)          tGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGa                                                                                                                           <  2:567064/140‑1 (MQ=255)               aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                                      <  2:308839/140‑1 (MQ=255)               aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                                      <  1:1086272/140‑1 (MQ=255)               aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                                      <  2:380479/140‑1 (MQ=255)                ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  1:610241/1‑140 (MQ=255)                ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  1:1267977/1‑140 (MQ=255)                ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  1:1369311/1‑140 (MQ=255)                ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCATGGATAAATACCAGCAATTGCTGGAAAAACACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  1:602082/1‑140 (MQ=255)                 cAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCa                                                                                                                    >  2:121872/1‑140 (MQ=255)                         gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            <  1:602903/140‑1 (MQ=255)                         gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            >  1:1039078/1‑140 (MQ=255)                         gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            <  2:1267977/140‑1 (MQ=255)                         gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCATGGATAAATACCAGCAATTGCTGGAAAAACACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            <  2:602082/140‑1 (MQ=255)                                   cGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCg                                                                                                  <  2:1039078/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGATGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 <  1:355240/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 >  1:745160/1‑140 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 <  1:464805/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 >  1:406027/1‑140 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 <  1:396595/140‑1 (MQ=255)                                    ggTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCgg                                                                                                 >  2:819937/1‑140 (MQ=255)                                      tATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTg                                                                                               <  1:999641/140‑1 (MQ=255)                                       aTCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTgg                                                                                              >  1:710780/1‑140 (MQ=255)                                       aTCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTgg                                                                                              >  1:236884/1‑140 (MQ=255)                                          tGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCt                                                                                           <  1:687404/140‑1 (MQ=255)                                          tGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCt                                                                                           >  2:1402017/1‑140 (MQ=255)                                           gCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCtt                                                                                          >  1:1474154/1‑140 (MQ=255)                                            cACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTa                                                                                         >  1:1019894/1‑140 (MQ=255)                                            cACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTa                                                                                         >  1:392637/1‑140 (MQ=255)                                            cACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTa                                                                                         >  1:256364/1‑140 (MQ=255)                                               gCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTaaaa                                                                                      >  1:231224/1‑140 (MQ=255)                                                cTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAAt                                                                                     <  1:165958/140‑1 (MQ=255)                                                cTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAAt                                                                                     >  1:242400/1‑140 (MQ=255)                                                cTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAAt                                                                                     >  2:704726/1‑140 (MQ=255)                                                cTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCCGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCATCTTTGATGACTTCAGCGGTGGGCTTAAAAt                                                                                     >  2:930083/1‑140 (MQ=255)                                                   gATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGAc                                                                                  >  2:22283/1‑140 (MQ=255)                                                   gATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGAc                                                                                  <  2:666684/140‑1 (MQ=255)                                                           ttCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTcgcg                                                                          <  2:30844/140‑1 (MQ=255)                                                           ttCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTcgcg                                                                          >  2:128315/1‑140 (MQ=255)                                                           ttCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTcgcg                                                                          <  1:1402017/140‑1 (MQ=255)                                                           ttCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTcgcg                                                                          >  2:789910/1‑140 (MQ=255)                                                             ccGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        <  2:610241/140‑1 (MQ=255)                                                             ccGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        >  1:1286252/1‑140 (MQ=255)                                                              cGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        >  1:772823/1‑139 (MQ=255)                                                              cGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        <  2:772823/139‑1 (MQ=255)                                                                ggcggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        >  2:596295/1‑137 (MQ=255)                                                                ggcggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        <  1:596295/137‑1 (MQ=255)                                                                  cggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGAt                                                                   <  1:704726/140‑1 (MQ=255)                                                                  cggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGAt                                                                   <  2:710780/140‑1 (MQ=255)                                                                  cggcTGGAAGTGATGTTGTTTACTGACGCCCCGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGAt                                                                   <  1:930083/140‑1 (MQ=255)                                                                   ggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATg                                                                  <  2:834605/140‑1 (MQ=255)                                                                   ggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATg                                                                  <  2:392637/140‑1 (MQ=255)                                                                      tGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGat                                                               >  2:818093/1‑140 (MQ=255)                                                                      tGGAAGTGATGTTGTTCACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGat                                                               >  1:312994/1‑140 (MQ=255)                                                                       ggAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGata                                                              <  2:1500591/140‑1 (MQ=255)                                                                       ggAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGata                                                              <  1:490327/140‑1 (MQ=255)                                                                              aTGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGa                                                       >  2:85192/1‑140 (MQ=255)                                                                                  tgttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGcc                                                   <  2:996365/140‑1 (MQ=255)                                                                                  tgttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGcc                                                   <  1:818093/140‑1 (MQ=255)                                                                                   gttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGccc                                                  <  2:236884/140‑1 (MQ=255)                                                                                    tttACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCg                                                 <  2:701319/140‑1 (MQ=255)                                                                                    tttACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCg                                                 >  2:629749/1‑140 (MQ=255)                                                                                       aCTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGa                                              <  2:1369311/140‑1 (MQ=255)                                                                                         tGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGaaa                                            >  2:635420/1‑140 (MQ=255)                                                                                           aCGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGaaaaa                                          >  1:1019549/1‑140 (MQ=255)                                                                                           aCGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGaaaaa                                          >  1:183718/1‑140 (MQ=255)                                                                                             gCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAata                                        >  2:521307/1‑140 (MQ=255)                                                                                             gCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAata                                        >  2:1390668/1‑140 (MQ=255)                                                                                             gCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAata                                        >  2:685649/1‑140 (MQ=255)                                                                                              cccTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAatat                                       >  2:1087818/1‑140 (MQ=255)                                                                                                      aaaTACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCgg                               <  2:312994/140‑1 (MQ=255)                                                                                                      aaaTACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCgg                               <  1:1087818/140‑1 (MQ=255)                                                                                                      aaaTACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCgg                               <  2:406027/140‑1 (MQ=255)                                                                                                      aaaTACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCgg                               <  2:1150403/140‑1 (MQ=255)                                                                                                       aaTACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACTAAGCCCGGGAAAAATATGCTCGCggg                              >  1:1161830/1‑140 (MQ=255)                                                                                                         tACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCt                            <  2:1019549/140‑1 (MQ=255)                                                                                                         tACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCt                            >  1:530984/1‑140 (MQ=255)                                                                                                             aGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCt                        >  2:1028290/1‑140 (MQ=255)                                                                                                             aGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCt                        >  2:389568/1‑140 (MQ=255)                                                                                                              gCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTa                       >  1:461313/1‑140 (MQ=255)                                                                                                                 aTTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAtct                    <  2:530984/140‑1 (MQ=255)                                                                                                                 aTTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAtct                    <  1:1390668/140‑1 (MQ=255)                                                                                                                  ttGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTAtctc                   >  2:755516/1‑140 (MQ=255)                                                                                                                     cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCt                <  1:755516/140‑1 (MQ=255)                                                                                                                       ggAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACTAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGa              <  2:1161830/140‑1 (MQ=255)                                                                                                                         aaaaaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACg            >  2:790508/1‑140 (MQ=255)                                                                                                                         aaaaaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACg            <  2:242400/140‑1 (MQ=255)                                                                                                                           aaaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGa          <  2:256364/140‑1 (MQ=255)                                                                                                                                   cATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCaaa  >  2:770864/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCAAA  >  NC_000913/208133‑208402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑