| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,676,056 | C→T | G102D (GGC→GAC) | pntB ← | pyridine nucleotide transhydrogenase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,676,056 | 0 | C | T | 98.2% | 182.4 / ‑5.3 | 54 | G102D (GGC→GAC) | pntB | pyridine nucleotide transhydrogenase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (31/22); total (31/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CACACAGTTTGCCGAACGCCACCACCGAACCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCA > NC_000913/1675919‑1676186 | cacacaGTTTGCCGAACGCCACCACCGAACCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCa < 1:693111/140‑1 (MQ=255) ttGCCGAACGCCACCACCGAACCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCAc < 1:1404538/140‑1 (MQ=255) tGCCGAACGCCACCACCGAACCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACt < 1:845007/140‑1 (MQ=255) aaCCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAg < 2:949815/140‑1 (MQ=255) aaCCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAg < 1:2738/140‑1 (MQ=255) aaCCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAg < 1:1188528/140‑1 (MQ=255) tGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGc < 2:500063/140‑1 (MQ=255) gAACGTTACCGCCCCGATGAAGATACCGAGGAACCCTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATGCCCGCGTCATGATGCAGATAGCTGTTAAAACCACCCAGCACTGCCGCCAGACCCACGAAGCTATGCa < 1:247007/140‑1 (MQ=255) tACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCg > 1:1446932/1‑140 (MQ=255) aTGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTc > 1:1351612/1‑140 (MQ=255) aCCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCAttt > 1:795696/1‑140 (MQ=255) ggAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTc > 2:1290992/1‑140 (MQ=255) aaCACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCAt > 1:1261369/1‑140 (MQ=255) aaCACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCAt > 2:834336/1‑140 (MQ=255) acacTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCAtt > 2:331705/1‑140 (MQ=255) ttCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCGCTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCaa < 1:1290992/140‑1 (MQ=255) ttCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCaa > 1:1263996/1‑140 (MQ=255) ttCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCaa < 2:1082298/140‑1 (MQ=255) cGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACtt > 1:565523/1‑140 (MQ=255) tCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTttc > 2:481489/1‑140 (MQ=255) tCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTttc > 2:82167/1‑140 (MQ=255) cAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTttct < 1:1264210/140‑1 (MQ=255) ggTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTttcttc > 2:912525/1‑140 (MQ=255) tGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGc > 2:1427141/1‑140 (MQ=255) tGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGc < 1:331705/140‑1 (MQ=255) aaTATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCa < 1:481489/140‑1 (MQ=255) aaTATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCa > 1:1223464/1‑140 (MQ=255) tatTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGa < 1:1066801/140‑1 (MQ=255) aCCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGAt > 2:717159/1‑140 (MQ=255) aCCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGAt > 2:683003/1‑140 (MQ=255) aCCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGAt > 2:599213/1‑140 (MQ=255) aCCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGAt > 2:1169282/1‑140 (MQ=255) aCCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGAt > 2:335237/1‑140 (MQ=255) aaTCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCaa > 2:1105765/1‑140 (MQ=255) aaTCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCaa > 1:1417361/1‑140 (MQ=255) gTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGcc > 2:35164/1‑122 (MQ=255) gTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGcc < 1:35164/122‑1 (MQ=255) gTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGcc < 1:1105765/140‑1 (MQ=255) gTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATA‑CAATTGccc < 2:1223464/140‑1 (MQ=255) tGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGccc < 2:1446932/140‑1 (MQ=255) tCCCGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCaa < 1:730161/140‑1 (MQ=255) ccGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGcc > 1:1083801/1‑131 (MQ=255) ccGCGTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGcc < 2:1083801/131‑1 (MQ=255) gcgTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGAc > 2:1186291/1‑140 (MQ=255) gTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGccc > 1:403614/1‑128 (MQ=255) gTCATGATGCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGccc < 2:403614/128‑1 (MQ=255) atgatgCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGccc > 2:57645/1‑125 (MQ=255) atgatgCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGccc < 1:57645/125‑1 (MQ=255) atgatgCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCg > 1:1497107/1‑140 (MQ=255) tgatgCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGc < 1:1186291/140‑1 (MQ=255) gatgCAGATAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGcc > 1:1075616/1‑140 (MQ=255) aTAGCTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGa > 1:1254938/1‑140 (MQ=255) cTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCa > 1:1153978/1‑140 (MQ=255) cTGTTAAAGTCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCa > 1:117958/1‑140 (MQ=255) | CACACAGTTTGCCGAACGCCACCACCGAACCCGTGAACGTTACCGCCCCGATGAAGATACCGAGGAACACTTCCGTCAGGTGAATATTGACCAGAATCGGTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCA > NC_000913/1675919‑1676186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |