Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,721,368	A→G	T35T (ACA→ACG)	ydhJ →	putative membrane fusion protein YdhJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,721,368	0	A	G	100.0%	272.2 / NA	77	T35T (ACA→ACG)	ydhJ	putative membrane fusion protein YdhJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/39);  total (38/39)

GCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTT  >  NC_000913/1721241‑1721502                                                                                                                                |                                                                                                                                       gCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGaaa                                                                                                                            >  1:921997/1‑140 (MQ=255)      aCTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATAcg                                                                                                                       >  1:805021/1‑140 (MQ=255)      aCTGATTGCGTGGTAACTATGTCAATTAAAAAAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATAcg                                                                                                                       >  2:509189/1‑140 (MQ=255)        tGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATAcgcg                                                                                                                     <  1:872162/140‑1 (MQ=255)        tGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATAcgcg                                                                                                                     <  2:70020/140‑1 (MQ=255)         gATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACgcgc                                                                                                                    >  1:1229122/1‑140 (MQ=255)           ttGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGa                                                                                                                                   <  1:109964/123‑1 (MQ=255)           ttGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGa                                                                                                                                   >  2:109964/1‑123 (MQ=255)           ttGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAg                                                                                                                  >  1:1293721/1‑140 (MQ=255)             gCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGaa                                                                                                                <  2:827641/140‑1 (MQ=255)             gCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGaa                                                                                                                <  2:921997/140‑1 (MQ=255)             gCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGaa                                                                                                                <  1:696888/140‑1 (MQ=255)              cGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAAc                                                                                                               <  2:9022/140‑1 (MQ=255)                    aaCTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATACAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGaa                                                                                                                <  1:263116/133‑1 (MQ=255)                    aaCTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATACAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGaa                                                                                                                >  2:263116/1‑133 (MQ=255)                        aTGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt                                                                                                     <  2:1293721/140‑1 (MQ=255)                        aTGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt                                                                                                     <  1:1148988/140‑1 (MQ=255)                        aTGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt                                                                                                     <  1:716188/140‑1 (MQ=255)                          gTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCa                                                                                                   <  2:532636/140‑1 (MQ=255)                           tCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAc                                                                                                  >  1:863623/1‑140 (MQ=255)                            cAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt                                                                                                     <  1:680740/136‑1 (MQ=255)                            cAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTAt                                                                                                     >  2:680740/1‑136 (MQ=255)                             aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCt                                                                                                       >  1:937477/1‑133 (MQ=255)                             aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCt                                                                                                       <  2:937477/133‑1 (MQ=255)                             aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAccc                                                                                                >  1:653189/1‑140 (MQ=255)                             aaTTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAccc                                                                                                >  2:1286093/1‑140 (MQ=255)                                tAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCcac                                                                                             <  2:1429497/140‑1 (MQ=255)                                   aaCAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCATAACAGGTTTCTATCACCCCACAgg                                                                                          >  2:861451/1‑140 (MQ=255)                                       aTTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTc                                                                                      <  2:653189/140‑1 (MQ=255)                                        ttAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCg                                                                                     <  1:566655/140‑1 (MQ=255)                                            gTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGAc                                                                                 >  2:1165260/1‑140 (MQ=255)                                                ttCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAt                                                                             >  2:530078/1‑140 (MQ=255)                                                     aacaaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgttg                                                                        >  2:955317/1‑140 (MQ=255)                                                       caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:1405845/140‑1 (MQ=255)                                                       caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:371545/140‑1 (MQ=255)                                                       caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:1229122/140‑1 (MQ=255)                                                       caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:1034458/140‑1 (MQ=255)                                                       caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  1:491624/140‑1 (MQ=255)                                                          tCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:850340/137‑1 (MQ=255)                                                          tCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      >  1:850340/1‑137 (MQ=255)                                                              tGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAata                                                               <  1:530078/140‑1 (MQ=255)                                                               gTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAatat                                                              <  1:1165260/140‑1 (MQ=255)                                                                   cGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      >  1:1160551/1‑128 (MQ=255)                                                                   cGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg                                                                      <  2:1160551/128‑1 (MQ=255)                                                                     gTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGa                                                        >  1:1459382/1‑140 (MQ=255)                                                                      tAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgtt                                                                         >  2:237545/1‑122 (MQ=255)                                                                      tAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgtt                                                                         <  1:237545/122‑1 (MQ=255)                                                                        gTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAg                                                         >  2:543785/1‑136 (MQ=255)                                                                        gTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAg                                                         <  1:543785/136‑1 (MQ=255)                                                                        gTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACaa                                                     >  2:240772/1‑140 (MQ=255)                                                                        gTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACaa                                                     >  2:293883/1‑140 (MQ=255)                                                                         ttGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAAc                                                    <  2:1072016/140‑1 (MQ=255)                                                                            cGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAg                                                 >  2:894632/1‑140 (MQ=255)                                                                               ttCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTg                                              >  2:773912/1‑140 (MQ=255)                                                                                    gCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAATGTTGAGCTGAATATAAAAGACAACCAGCTGGTTaa                                         >  1:1350614/1‑140 (MQ=255)                                                                                       ggATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAAcgc                                      <  2:270245/140‑1 (MQ=255)                                                                                         aTGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgg                                    <  1:894632/140‑1 (MQ=255)                                                                                            gtggCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCggggg                                 <  1:110691/140‑1 (MQ=255)                                                                                            gtggCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCggggg                                 <  1:203781/140‑1 (MQ=255)                                                                                               gCTGTGGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtc                              >  1:1153848/1‑140 (MQ=255)                                                                                                 tgtgGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtctt                            <  1:240772/140‑1 (MQ=255)                                                                                                 tgtgGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtctt                            <  1:509189/140‑1 (MQ=255)                                                                                                  gtgGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtctt                            >  2:539835/1‑139 (MQ=255)                                                                                                  gtgGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtctt                            <  1:539835/139‑1 (MQ=255)                                                                                                   tgGAATTATTACATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtcttct                          >  1:822852/1‑140 (MQ=255)                                                                                                        ttattaCATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCa                     <  1:1286093/140‑1 (MQ=255)                                                                                                             aCATGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCAcgatc                <  2:1459382/140‑1 (MQ=255)                                                                                                               aTGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCAcgatcga              >  1:93179/1‑140 (MQ=255)                                                                                                                tGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCAcgatcgat             >  1:126741/1‑140 (MQ=255)                                                                                                                tGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCAcgatcgat             >  2:1273489/1‑140 (MQ=255)                                                                                                                tGCAATCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCAcgatcgat             >  1:852647/1‑140 (MQ=255)                                                                                                                   aaTCTCCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATaaa          >  2:572011/1‑140 (MQ=255)                                                                                                                     tctcCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAAc        >  2:596756/1‑140 (MQ=255)                                                                                                                     tctcCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAAc        >  2:509779/1‑140 (MQ=255)                                                                                                                     tctcCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAAc        >  1:993502/1‑140 (MQ=255)                                                                                                                      ctcCGTGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACg       <  1:293883/140‑1 (MQ=255)                                                                                                                           tGGACGCGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCtt  >  2:380827/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                       GCAATACTGATTGCGTGGTAACTATGTCAATTAAAACAATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCTTCTGCTCACGATCGATAAAACGCCCTT  >  NC_000913/1721241‑1721502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑