Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,791	T→C	V136A (GTT→GCT)	cdgI →	putative c‑di‑GMP binding protein CdgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,791	0	T	C	100.0%	317.2 / NA	88	V136A (GTT→GCT)	cdgI	putative c‑di‑GMP binding protein CdgI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/53);  total (35/53)

CTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTA  >  NC_000913/1870655‑1870926                                                                                                                                         |                                                                                                                                        cTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGa                                                                                                                                      <  2:123370/140‑1 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGag                                                                                                                                     <  1:1031011/140‑2 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     >  2:1169748/1‑140 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     <  1:1218409/140‑1 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     >  1:443221/1‑140 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     >  2:626889/1‑140 (MQ=255)  tGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACCCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     >  2:1508844/1‑140 (MQ=255)   gATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaaa                                                                                                                                    <  2:345833/140‑1 (MQ=255)   gATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaaa                                                                                                                                    >  1:259952/1‑140 (MQ=255)    aTGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAAc                                                                                                                                   >  2:1457115/1‑140 (MQ=255)    aTGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAAc                                                                                                                                   <  1:850043/140‑1 (MQ=255)    aTGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAAc                                                                                                                                   >  2:532222/1‑140 (MQ=255)    aTGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAAc                                                                                                                                   <  1:161044/140‑1 (MQ=255)      ggTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCa                                                                                                                                 <  1:3236/140‑1 (MQ=255)        ttgttgTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCAtt                                                                                                                               >  2:1216995/1‑140 (MQ=255)         tgttgtGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTg                                                                                                                              <  1:1457115/140‑1 (MQ=255)         tgttgtGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTg                                                                                                                              <  1:1169748/140‑1 (MQ=255)          gttgtGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGt                                                                                                                             >  2:965553/1‑140 (MQ=255)          gttgtGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGt                                                                                                                             >  2:764725/1‑140 (MQ=255)          gttgtGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGt                                                                                                                             >  2:623317/1‑140 (MQ=255)              tgGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAAtt                                                                                                                         <  1:626889/140‑1 (MQ=255)              tgGCTTTGATTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAAtt                                                                                                                         >  2:1116366/1‑140 (MQ=255)                  tttGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     >  2:722375/1‑124 (MQ=255)                  tttGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGaa                                                                                                                                     <  1:722375/124‑1 (MQ=255)                  tttGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATcc                                                                                                                     >  1:462874/1‑140 (MQ=255)                    tGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCAt                                                                                                                   >  2:1424929/1‑140 (MQ=255)                      tttttCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTAAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATTCATaa                                                                                                                 <  2:11521/140‑1 (MQ=255)                        tttCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAAc                                                                                                               <  1:953950/140‑1 (MQ=255)                        tttCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAAc                                                                                                               <  1:1424929/140‑1 (MQ=255)                          tCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCa                                                                                                             >  1:614591/1‑140 (MQ=255)                           ccTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCaa                                                                                                            <  2:259952/140‑1 (MQ=255)                             ttAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAAtt                                                                                                          <  2:410825/140‑1 (MQ=255)                              tAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAAtt                                                                                                          >  2:86187/1‑139 (MQ=255)                              tAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAAtt                                                                                                          <  1:86187/139‑1 (MQ=255)                               aaCGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTaa                                                                                                        >  2:1419529/1‑140 (MQ=255)                               aaCGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTaa                                                                                                        <  1:735653/140‑1 (MQ=255)                               aaCGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTaa                                                                                                        <  1:276473/140‑1 (MQ=255)                               aaCGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTaa                                                                                                        >  2:276473/1‑140 (MQ=255)                               aaCGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTaa                                                                                                        <  1:551268/140‑1 (MQ=255)                                  gCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACgg                                                                                                     <  1:623317/140‑1 (MQ=255)                                  gCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACgg                                                                                                     <  2:1369049/140‑1 (MQ=255)                                  gCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACgg                                                                                                     <  1:965553/140‑1 (MQ=255)                                    cGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCa                                                                                                   <  1:532222/140‑1 (MQ=255)                                    cGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCa                                                                                                   <  1:764725/140‑1 (MQ=255)                                    cGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCa                                                                                                   <  1:39743/140‑1 (MQ=255)                                     gTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAg                                                                                                  <  1:248643/140‑1 (MQ=255)                                     gTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAg                                                                                                  <  2:462874/140‑1 (MQ=255)                                         tttttCTTTTCATGTTCATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTaca                                                                                              <  2:1362233/140‑1 (MQ=255)                                         tttttCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTaca                                                                                              <  2:186404/140‑1 (MQ=255)                                         tttttCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTaca                                                                                              <  1:1018828/140‑1 (MQ=255)                                         tttttCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTaca                                                                                              <  2:1400787/140‑1 (MQ=255)                                         tttttCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTaca                                                                                              <  2:630191/140‑1 (MQ=255)                                              cttttcATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGAt                                                                                         <  1:713855/140‑1 (MQ=255)                                                   cATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGa                                                                                    <  2:613530/140‑1 (MQ=255)                                                         aaTGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACTATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGt                                                                              >  1:925899/1‑140 (MQ=255)                                                         aaTGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGt                                                                              >  1:996874/1‑140 (MQ=255)                                                           tGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCg                                                                            >  1:400738/1‑140 (MQ=255)                                                              aaTATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAat                                                                         <  2:996874/140‑1 (MQ=255)                                                              aaTATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAat                                                                         <  1:1508844/140‑1 (MQ=255)                                                                tatTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGaa                                                                          >  2:714859/1‑137 (MQ=255)                                                                tatTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGaa                                                                          <  1:714859/137‑1 (MQ=255)                                                                     cACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGttt                                                                  <  1:1419529/140‑1 (MQ=255)                                                                      aCTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGtttc                                                                 >  2:572213/1‑140 (MQ=255)                                                                       cTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGtttct                                                                <  2:1426195/140‑1 (MQ=255)                                                                          acaaacaaATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGtttctatt                                                             <  2:817502/140‑1 (MQ=255)                                                                               caaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACTATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGtttctattttcta                                                        <  2:925899/140‑1 (MQ=255)                                                                               caaaTTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGtttctattttcta                                                        <  1:1116366/140‑1 (MQ=255)                                                                                    tttACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                   >  2:1040311/1‑140 (MQ=255)                                                                                    tttACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATAttt                                                   <  1:510634/140‑1 (MQ=255)                                                                                             acTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAAc                                          >  1:1312480/1‑140 (MQ=255)                                                                                               tCATTTTAAGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTc                                        <  2:1312480/140‑1 (MQ=255)                                                                                                       aGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTAt                                <  2:400738/140‑1 (MQ=255)                                                                                                       aGCCTTGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTAt                                >  1:68767/1‑140 (MQ=255)                                                                                                           ttGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGt                            >  2:586919/1‑140 (MQ=255)                                                                                                            tGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGtt                           >  2:1287813/1‑140 (MQ=255)                                                                                                            tGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGtt                           >  2:579938/1‑140 (MQ=255)                                                                                                            tGCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGtt                           >  1:855/1‑140 (MQ=255)                                                                                                             gCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt                          >  2:23283/1‑140 (MQ=255)                                                                                                             gCATTTTTAAGTGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGttt                          >  1:120807/1‑140 (MQ=255)                                                                                                                        tGGCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTgg               <  2:327583/140‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAAc                       <  1:812304/130‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAAc                       >  2:812304/1‑130 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcg             <  2:68767/140‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcg             <  1:400221/140‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcg             <  1:662087/140‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcg             <  1:1216995/140‑1 (MQ=255)                                                                                                                          gCCTTACCTATTTAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGgcg             <  1:586919/140‑1 (MQ=255)                                                                                                                                     ttAGCTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTa  >  2:855903/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTGATGGTTGTTGTGGCTTTGTTTTTCCTTAACGCCGTCATTTTTCTTTTCATGTTAATGAAATATTTCACTAACAAACAAATTTTACCAACACTCATTTTAAGCCTTGCATTTTTAAGTGGCCTTATCTATTTAGTTGAAACCATTGTAATTATCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTA  >  NC_000913/1870655‑1870926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑