Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,901,041	T→C	H182R (CAC→CGC)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,901,041	0	T	C	100.0%	246.5 / NA	75	H182R (CAC→CGC)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/33);  total (42/33)

CACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAC  >  NC_000913/1900908‑1901174                                                                                                                                      |                                                                                                                                      cacTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGt                                                                                                                                 >  2:648107/1‑140 (MQ=255)      cGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACg                                                                                                                            >  2:254037/1‑140 (MQ=255)         tgatgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAAt                                                                                                                         <  1:354093/140‑1 (MQ=255)           atgatCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATcg                                                                                                                       <  2:607328/140‑1 (MQ=255)              atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGt                                                                                                                    >  1:1162252/1‑140 (MQ=255)              atCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGt                                                                                                                    >  1:1345859/1‑140 (MQ=255)                      gCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGtt                                                                                                            <  1:115472/140‑1 (MQ=255)                       ccAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGttt                                                                                                           >  1:1062764/1‑140 (MQ=255)                        cAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTg                                                                                                          >  1:1389938/1‑140 (MQ=255)                         aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTgg                                                                                                         >  1:319010/1‑140 (MQ=255)                         aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTgg                                                                                                         >  2:208759/1‑140 (MQ=255)                          aTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                        >  2:347718/1‑140 (MQ=255)                          aTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                        >  2:644564/1‑140 (MQ=255)                          aTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGa                                                                                                        >  2:72950/1‑140 (MQ=255)                              cgGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                    <  1:736032/140‑1 (MQ=255)                              cgGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                    <  1:648107/140‑1 (MQ=255)                                  aTACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCa                                                                                                <  2:1352056/140‑1 (MQ=255)                                        gTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATaa                                                                                          >  1:290682/1‑140 (MQ=255)                                        gTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTAGATGCCAGCAACATaa                                                                                          >  2:1346106/1‑140 (MQ=255)                                           aCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAAccc                                                                                       >  2:35203/1‑139 (MQ=255)                                           aCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCg                                                                                       >  1:1094535/1‑140 (MQ=255)                                            ccTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGc                                                                                      <  2:1094535/140‑1 (MQ=255)                                             cTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGcc                                                                                     >  2:1286383/1‑140 (MQ=255)                                             cTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGcc                                                                                     >  2:975678/1‑140 (MQ=255)                                              tttCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCa                                                                                    <  2:1389938/140‑1 (MQ=255)                                                 cGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCg                                                                                 <  1:347718/140‑1 (MQ=255)                                                 cGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCg                                                                                 <  1:525806/140‑1 (MQ=255)                                                  ggAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGc                                                                                >  1:572294/1‑140 (MQ=255)                                                   gAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGACCACCCCCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCa                                                                               <  2:180479/140‑1 (MQ=255)                                                       gtgGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCa                                                                           >  2:1248676/1‑140 (MQ=255)                                                           aaaccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAc                                                                       <  2:572294/140‑1 (MQ=255)                                                                gaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTAccgccg                                                                  <  1:208759/140‑1 (MQ=255)                                                                          gCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCAc                                                        <  2:365623/140‑1 (MQ=255)                                                                          cccaccaGACTCAGCCCAATCATTAACAGGAAGCTCAGCCAGAGCACCACCACCGTGGGGCGCTGGTTAACGAACCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCAc                                                        <  2:319010/139‑1 (MQ=255)                                                                           ccaccaGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCAcc                                                       >  2:1088184/1‑140 (MQ=255)                                                                               caGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                   <  1:1286383/140‑1 (MQ=255)                                                                                  aCTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGAt                                                >  1:1489685/1‑140 (MQ=255)                                                                                    tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCATTCACTACCGCCGCCATCATCACCGGCAGATgg                                              <  1:72950/140‑1 (MQ=255)                                                                                    tCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATgg                                              <  1:644564/140‑1 (MQ=255)                                                                                       gACCAATCATTAACAGGAAGCTCAGACAGAGCACCCCCACCGTGGGGCGCTGGTTAACGAATCGCGCCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTa                                           <  2:582808/140‑1 (MQ=255)                                                                                       gACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTa                                           <  1:1248676/140‑1 (MQ=255)                                                                                          cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAcc                                        <  1:1294347/140‑1 (MQ=255)                                                                                          cAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAcc                                        <  1:254037/140‑1 (MQ=255)                                                                                            aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGAt                                                <  2:1169533/130‑1 (MQ=255)                                                                                            aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGAt                                                >  1:1169533/1‑130 (MQ=255)                                                                                            aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCAt                                      <  2:1162252/140‑1 (MQ=255)                                                                                            aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCAt                                      >  2:566077/1‑140 (MQ=255)                                                                                            aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCAt                                      >  2:275463/1‑140 (MQ=255)                                                                                             tCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                   <  1:336289/126‑1 (MQ=255)                                                                                             tCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCa                                                   >  2:336289/1‑126 (MQ=255)                                                                                             tCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAg                                                  <  1:651856/127‑1 (MQ=255)                                                                                             tCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAg                                                  >  2:651856/1‑127 (MQ=255)                                                                                               aTTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                   <  1:275463/140‑1 (MQ=255)                                                                                                       gAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAc                                         <  2:597378/126‑1 (MQ=255)                                                                                                       gAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAAc                                         >  1:597378/1‑126 (MQ=255)                                                                                                       gAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAg                           >  1:1216139/1‑140 (MQ=255)                                                                                                       gAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAg                           >  1:1021321/1‑140 (MQ=255)                                                                                                       gAAGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAg                           >  1:1436847/1‑140 (MQ=255)                                                                                                         aGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                   >  1:1103484/1‑130 (MQ=255)                                                                                                         aGCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                   <  2:1103484/130‑1 (MQ=255)                                                                                                          gCTCAGACAGAGCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTaa                        <  2:1345859/140‑1 (MQ=255)                                                                                                                  agagCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCa                <  1:1088184/140‑1 (MQ=255)                                                                                                                    agCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCa                <  1:528891/138‑1 (MQ=255)                                                                                                                    agCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCa                >  2:528891/1‑138 (MQ=255)                                                                                                                    agCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCAt               <  2:571218/139‑1 (MQ=255)                                                                                                                    agCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCAt               >  1:571218/1‑139 (MQ=255)                                                                                                                    agCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATc              >  1:724599/1‑140 (MQ=255)                                                                                                                     gCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc             >  2:1256784/1‑140 (MQ=255)                                                                                                                     gCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc             >  2:1162793/1‑140 (MQ=255)                                                                                                                     gCACCACCACCGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc             >  2:1131537/1‑140 (MQ=255)                                                                                                                       cccaccaccGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCaa           <  2:1489685/139‑1 (MQ=255)                                                                                                                         caccaccGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACg         >  1:1293463/1‑140 (MQ=255)                                                                                                                         caccaccGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACg         >  1:318760/1‑140 (MQ=255)                                                                                                                              ccGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGagaag    >  1:646422/1‑140 (MQ=255)                                                                                                                               cGTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGagaaga   >  1:1402257/1‑140 (MQ=255)                                                                                                                                gTGGGGCGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAc  >  1:1273693/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      CACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAAGAC  >  NC_000913/1900908‑1901174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑