Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,174,939 T→C P37P (CCA→CCG)  gatA ← galactitol‑specific PTS enzyme IIA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,174,9390TC100.0% 307.9 / NA 85P37P (CCA→CCG) gatAgalactitol‑specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/44);  total (41/44)

CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGCACAG  >  NC_000913/2174806‑2175071
                                                                                                                                     |                                                                                                                                    
cTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAg                                                                                                                                <  1:983383/140‑1 (MQ=255)
    aTTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTAt                                                                                                                            >  2:1255394/1‑140 (MQ=255)
      ttgttgGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCa                                                                                                                          <  1:1203251/140‑1 (MQ=255)
      ttgttgGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCa                                                                                                                          <  2:286953/140‑1 (MQ=255)
      ttgttgGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCa                                                                                                                          <  2:781042/140‑1 (MQ=255)
           ggCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAAc                                                                                                                     >  2:879004/1‑140 (MQ=255)
             ccTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACca                                                                                                                   >  2:154972/1‑140 (MQ=255)
             ccTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACca                                                                                                                   >  2:586628/1‑140 (MQ=255)
             ccTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACca                                                                                                                   <  1:571000/140‑1 (MQ=255)
             ccTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACca                                                                                                                   <  1:1321111/140‑1 (MQ=255)
             ccTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACca                                                                                                                   <  1:398727/140‑1 (MQ=255)
                tAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCacac                                                                                                                <  2:847657/140‑1 (MQ=255)
                 aaCAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACAcc                                                                                                               >  2:661982/1‑140 (MQ=255)
                 aaCAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACAcc                                                                                                               >  1:230261/1‑140 (MQ=255)
                     gATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTg                                                                                                           <  1:1211803/140‑1 (MQ=255)
                      aTAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTgg                                                                                                          >  1:818139/1‑140 (MQ=255)
                      aTAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTgg                                                                                                          >  2:971971/1‑140 (MQ=255)
                       tAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGc                                                                                                         <  2:1345845/140‑1 (MQ=255)
                          aTGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      <  1:879004/140‑1 (MQ=255)
                          aTGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      <  1:971971/140‑1 (MQ=255)
                          aTGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      <  2:265463/140‑1 (MQ=255)
                          aTGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      <  1:586628/140‑1 (MQ=255)
                           tGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGc                                                                                                     <  2:631929/140‑1 (MQ=255)
                            ggCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCa                                                                                                    <  2:810295/140‑1 (MQ=255)
                            ggCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCa                                                                                                    >  1:1185571/1‑140 (MQ=255)
                            ggCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCa                                                                                                    <  2:75409/140‑1 (MQ=255)
                             gCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACCCCTTTGGCGAGCAt                                                                                                   <  1:1147304/140‑1 (MQ=255)
                             gCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCAt                                                                                                   >  2:1147304/1‑140 (MQ=255)
                                 acgacTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      >  2:360252/1‑133 (MQ=255)
                                 acgacTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAg                                                                                                      <  1:360252/133‑1 (MQ=255)
                                  cgacTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCAt                                                                                              <  1:154972/140‑1 (MQ=255)
                                   gacTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCAtt                                                                                             <  1:419681/140‑1 (MQ=255)
                                   gacTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCAtt                                                                                             <  1:883684/140‑1 (MQ=255)
                                      ttAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTAcc                                                                                          >  2:842812/1‑140 (MQ=255)
                                          cATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGata                                                                                      <  2:230261/140‑1 (MQ=255)
                                          cATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGata                                                                                      <  1:661982/140‑1 (MQ=255)
                                               aTCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGata                                                                                      >  1:1216626/1‑135 (MQ=255)
                                               aTCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGata                                                                                      <  2:1216626/135‑1 (MQ=255)
                                                   ccTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTaaa                                                                             >  1:1401393/1‑140 (MQ=255)
                                                     tCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAAc                                                                           <  2:1185571/140‑1 (MQ=255)
                                                             cGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTAc                                                                   >  2:499134/1‑140 (MQ=255)
                                                                       aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACaaa                                                         <  2:1448773/140‑1 (MQ=255)
                                                                       aTAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACaaa                                                         >  2:1011763/1‑140 (MQ=255)
                                                                          gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGa                                                      >  1:744721/1‑140 (MQ=255)
                                                                          gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGa                                                      >  1:436458/1‑140 (MQ=255)
                                                                          gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGa                                                      >  1:439057/1‑140 (MQ=255)
                                                                          gCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGa                                                      >  1:660013/1‑140 (MQ=255)
                                                                           cGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGaa                                                     >  1:960628/1‑140 (MQ=255)
                                                                            gTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGaaa                                                    >  2:231556/1‑140 (MQ=255)
                                                                             tgctgcTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAAt                                                   >  2:110278/1‑140 (MQ=255)
                                                                             tgctgcTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAAt                                                   >  2:765939/1‑140 (MQ=255)
                                                                             tgctgcTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAAt                                                   >  2:1304138/1‑140 (MQ=255)
                                                                             tgctgcTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAAt                                                   >  2:1424546/1‑140 (MQ=255)
                                                                             tgctgcTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAAt                                                   >  2:187218/1‑140 (MQ=255)
                                                                               ctgctCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTc                                                 >  2:1472634/1‑140 (MQ=255)
                                                                                  ctCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGc                                              >  2:1098120/1‑140 (MQ=255)
                                                                                  ctCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGc                                              >  2:159956/1‑140 (MQ=255)
                                                                                      aGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACg                                          <  1:1304138/140‑1 (MQ=255)
                                                                                          ttATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGaaca                                      <  1:1011763/140‑1 (MQ=255)
                                                                                            aTCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGaacaaa                                    >  2:992771/1‑140 (MQ=255)
                                                                                                 ggTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGt                               <  1:765939/140‑1 (MQ=255)
                                                                                                  gTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGtt                              >  1:717366/1‑140 (MQ=255)
                                                                                                  gTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGAACGACAAAAGAAATTCCGCTACGAACAAACAGGtt                              >  2:1322849/1‑140 (MQ=255)
                                                                                                   tAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTa                             >  1:982177/1‑140 (MQ=255)
                                                                                                   tAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTa                             >  1:121196/1‑140 (MQ=255)
                                                                                                   tAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTa                             <  1:477326/140‑1 (MQ=255)
                                                                                                   gtaggAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCt                                             >  2:405111/4‑124 (MQ=255)
                                                                                                   gtaggAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCt                                             <  1:405111/121‑1 (MQ=255)
                                                                                                     gggAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGt                           <  2:744721/140‑1 (MQ=255)
                                                                                                      ggAATTCTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTc                          <  2:121196/140‑1 (MQ=255)
                                                                                                            cTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATAc                    <  1:110278/140‑1 (MQ=255)
                                                                                                            cTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATAc                    <  1:231556/140‑1 (MQ=255)
                                                                                                            cTGCTTCTCTGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATAc                    <  2:660013/140‑1 (MQ=255)
                                                                                                                  ctctGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGt                               >  1:374579/1‑123 (MQ=255)
                                                                                                                  ctctGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGt                               <  2:374579/123‑1 (MQ=255)
                                                                                                                  ctctGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCt              >  2:515770/1‑140 (MQ=255)
                                                                                                                  ctctGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCt              >  2:1356509/1‑140 (MQ=255)
                                                                                                                     tGGCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTAt           >  1:72378/1‑140 (MQ=255)
                                                                                                                       gCAATTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTc         >  1:654359/1‑140 (MQ=255)
                                                                                                                          aTTAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGc      <  1:1255394/140‑1 (MQ=255)
                                                                                                                           ttAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGca     <  2:622173/140‑1 (MQ=255)
                                                                                                                           ttAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGca     <  1:1472634/140‑1 (MQ=255)
                                                                                                                           ttAATGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGca     <  2:960628/140‑1 (MQ=255)
                                                                                                                              aTGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGCACAg  <  1:499134/140‑1 (MQ=255)
                                                                                                                              aTGCCTGCGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGCACAg  <  2:439057/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTGCTTCTCTGGCAATTAATGCCTGTGGCCAGGTATCATGAACCACACCTTTGGCGAGCATCTCATTACCGATATGGGTTAAAACTTCGCTACGATCGACAAAAGAAATTCCGCTACGAACAAACAGGTTAGTCATATACCGTCCTTATTCCGCACAG  >  NC_000913/2174806‑2175071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑