Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,258,987	T→C	T104A (ACC→GCC)	psuK ←	putative pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,258,987	0	T	C	100.0%	341.3 / NA	95	T104A (ACC→GCC)	psuK	putative pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (56/39);  total (56/39)

GCCTCTTCACTGATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGTATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGC  >  NC_000913/2258854‑2259123                                                                                                                                      |                                                                                                                                         gCCTCTTCACTGATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATc                                                                                                                                    <  2:427252/140‑1 (MQ=255)    tctTCACTGATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAg                                                                                                                                 <  1:850178/140‑1 (MQ=255)    tctTCACTGATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAg                                                                                                                                 <  1:214288/140‑1 (MQ=255)            gATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTa                                                                                                                               >  2:1478575/1‑134 (MQ=255)            gATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTa                                                                                                                               <  1:1478575/134‑1 (MQ=255)            gATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGata                                                                                                                         <  2:982703/140‑1 (MQ=255)             atatTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAg                                                                                                                                 >  1:702787/1‑131 (MQ=255)             atatTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAg                                                                                                                                 <  2:702787/131‑1 (MQ=255)             atatTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGataa                                                                                                                        <  2:1132740/140‑1 (MQ=255)             atatTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGataa                                                                                                                        >  1:1198811/1‑140 (MQ=255)              tatTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGataaa                                                                                                                       <  2:1456170/140‑1 (MQ=255)                   cAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACt                                                                                                                  <  1:1100927/140‑1 (MQ=255)                       ccGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGAc                                                                                                              >  1:400471/1‑140 (MQ=255)                       ccGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGAc                                                                                                              >  1:662192/1‑140 (MQ=255)                       ccGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGGGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGAc                                                                                                              >  1:358792/1‑140 (MQ=255)                        cgcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACg                                                                                                             >  2:591656/1‑140 (MQ=255)                        cgcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACg                                                                                                             >  1:631569/1‑140 (MQ=255)                        cgcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACg                                                                                                             >  2:475021/1‑140 (MQ=255)                        cgcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACg                                                                                                             <  2:414883/140‑1 (MQ=255)                        cgcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACg                                                                                                             >  1:414883/1‑140 (MQ=255)                         gcgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGt                                                                                                            >  1:446857/1‑140 (MQ=255)                          cgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTa                                                                                                           >  2:188346/1‑140 (MQ=255)                          cgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTa                                                                                                           >  2:1496536/1‑140 (MQ=255)                          cgACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTa                                                                                                           >  2:1115013/1‑140 (MQ=255)                           gACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTAt                                                                                                          <  2:41527/140‑1 (MQ=255)                              aaTGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTAtttt                                                                                                       >  2:1008901/1‑140 (MQ=255)                                tGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTtct                                                                                                     <  1:565334/140‑1 (MQ=255)                                  aCCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGa                                                                                                               >  1:96715/1‑128 (MQ=255)                                  aCCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGa                                                                                                               <  2:96715/128‑1 (MQ=255)                                     tttGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCgg                                                                                                <  1:1008901/140‑1 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              >  2:474342/1‑140 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              >  1:987202/1‑140 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              >  2:998418/1‑140 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              <  2:1201653/140‑1 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              >  2:444919/1‑140 (MQ=255)                                       tGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGca                                                                                              >  1:799899/1‑140 (MQ=255)                                           ctctGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAAt                                                                                          <  1:1496536/140‑1 (MQ=255)                                              tGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAg                                                                                       >  2:1158106/1‑140 (MQ=255)                                              tGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGATGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAg                                                                                       >  2:912976/1‑140 (MQ=255)                                               gAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAgg                                                                                      <  1:513094/140‑1 (MQ=255)                                                     aTTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTATCCCGGCACAATCAGGCATTTa                                                                                >  2:1477231/1‑140 (MQ=255)                                                        cACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTa                                                                                >  2:1338383/1‑137 (MQ=255)                                                        cACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTa                                                                                <  1:1338383/137‑1 (MQ=255)                                                          ccGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGAc                                                                           >  2:1149973/1‑140 (MQ=255)                                                             tGCTGTGCGAGATATTCAGCTGTAATAGCGATGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATa                                                                        <  1:912976/140‑1 (MQ=255)                                                              gCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATaa                                                                       >  2:868829/1‑140 (MQ=255)                                                               cTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATaaa                                                                      >  2:604414/1‑140 (MQ=255)                                                               cTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATaaa                                                                      >  1:1014733/1‑140 (MQ=255)                                                               cTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATaaa                                                                      <  1:474342/140‑1 (MQ=255)                                                                  tgCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGc                                                                   >  1:894571/1‑140 (MQ=255)                                                                   gCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGcc                                                                  <  2:400471/140‑1 (MQ=255)                                                                       gaTATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAgat                                                              >  1:1300357/1‑140 (MQ=255)                                                                              aGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTgg                                                       <  1:604414/140‑1 (MQ=255)                                                                              aGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTgg                                                       <  2:799899/140‑1 (MQ=255)                                                                                 tGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGttt                                                    >  1:1213773/1‑140 (MQ=255)                                                                                     aTAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGt                                                >  2:809928/1‑140 (MQ=255)                                                                                        gCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTag                                             <  1:591656/140‑1 (MQ=255)                                                                                          gTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTagca                                           >  1:643049/1‑140 (MQ=255)                                                                                             gCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCTTTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCg                                        >  2:406431/1‑140 (MQ=255)                                                                                             gCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTgg                                                       >  2:39640/1‑125 (MQ=255)                                                                                             gCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTgg                                                       <  1:39640/125‑1 (MQ=255)                                                                                             gCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCg                                        >  2:195490/1‑140 (MQ=255)                                                                                              cTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGa                                       >  1:1315234/1‑140 (MQ=255)                                                                                              cTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGa                                       <  2:1014733/140‑1 (MQ=255)                                                                                              cTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGa                                       >  2:254924/1‑140 (MQ=255)                                                                                                  tatTCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGa                                   <  2:643049/140‑1 (MQ=255)                                                                                                    ttCATGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGAcc                                 <  2:1000610/140‑1 (MQ=255)                                                                                                       aTGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATa                              >  1:328243/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGa                                       >  2:620506/1‑130 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGa                                       <  1:620506/130‑1 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  2:189214/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  1:416840/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  2:433282/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  1:306373/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  1:1306948/1‑140 (MQ=255)                                                                                                        tGTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaa                             >  2:1111267/1‑140 (MQ=255)                                                                                                         gTCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaaa                            >  2:1131944/1‑140 (MQ=255)                                                                                                          tCATTTATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATaaaa                           >  1:592058/1‑140 (MQ=255)                                                                                                             tttATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATc                        <  2:1198811/140‑1 (MQ=255)                                                                                                              ttATAGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCTTTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCa                       <  1:406431/140‑1 (MQ=255)                                                                                                                ataGCAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACt                     <  1:189214/140‑1 (MQ=255)                                                                                                                    cAACCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCt                 >  2:1067573/1‑140 (MQ=255)                                                                                                                     aaCCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTa                >  2:815653/1‑140 (MQ=255)                                                                                                                      aCCAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTAc               >  2:1009558/1‑140 (MQ=255)                                                                                                                       ccAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACg              >  1:249394/1‑140 (MQ=255)                                                                                                                       ccAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACg              >  2:836209/1‑140 (MQ=255)                                                                                                                       ccAGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACg              >  1:1218013/1‑140 (MQ=255)                                                                                                                         aGCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGgc            <  1:1067573/140‑1 (MQ=255)                                                                                                                          gCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGgcg           <  2:1218013/140‑1 (MQ=255)                                                                                                                          gCATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGgcg           <  1:1158106/140‑1 (MQ=255)                                                                                                                           cATTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGgcgc          >  1:965082/1‑140 (MQ=255)                                                                                                                            aTTTCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACACCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCt         <  1:1348819/140‑1 (MQ=255)                                                                                                                             tttCACCGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTc        <  1:809928/140‑1 (MQ=255)                                                                                                                                  ccGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAg   <  1:868829/140‑1 (MQ=255)                                                                                                                                   cGGCATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGc  <  2:416840/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GCCTCTTCACTGATATTACAGTCCGCGACAATGACCTTTGCCCTCTGAATAAATTCACCGTGCTGTGCGAGATATTCAGCTGTAATAGCGTTGCTAATATTCATGTCATTTATAGCAACCAGCATTTCACCGGTATTATCGAGTAATGATAAATAACTCGACGTATTTTCTCCCGGCACAATCAGGCATTTATCGACATAAACGCCAGATTGATTGGTTTGCGTTAGCAGCGATTGACCATAAAAATCACTGCCTACGGCGCTCAGTAGC  >  NC_000913/2258854‑2259123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑