BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,280,240	T→C	T82A (ACG→GCG)	rsuA ←	16S rRNA pseudouridine(516) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,280,240	0	T	C	100.0%	370.4 / NA	103	T82A (ACG→GCG)	rsuA	16S rRNA pseudouridine(516) synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (59/44); total (59/44)

Only 100 of 104 total aligned reads displayed.

ATGATGGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGTTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGC  >  NC_000913/2280103‑2280375

atgatgGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTg                                                                                                                                       >  1:910237/1‑140 (MQ=255)

 ggagggCGGGGAGAAGTAAGGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGCCGGGTGGTATAAATACCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTgg                                                                                                                                      <  2:736421/136‑1 (MQ=255)

   atgGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGt                                                                                                                                    <  2:673020/140‑1 (MQ=255)

    tgGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTg                                                                                                                                   >  1:472574/1‑140 (MQ=255)

    tgGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTg                                                                                                                                   >  1:529835/1‑140 (MQ=255)

     ggCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGa                                                                                                                                  >  2:1455504/1‑140 (MQ=255)

     ggCGCGGAGAAGAAATGGGGTGCGCCCACTGACCATCACCAGCCATCAGCACCAGCCCGGTGGTATCAATACCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGa                                                                                                                                  <  2:1415362/140‑1 (MQ=255)

      gcgcGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGAt                                                                                                                                 >  1:575508/1‑140 (MQ=255)

       cgcgGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATc                                                                                                                                >  2:1215070/1‑140 (MQ=255)

       cgcgGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATc                                                                                                                                >  1:1145468/1‑140 (MQ=255)

                 tAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTcc                                                                                                                      >  2:310040/1‑140 (MQ=255)

                  aaTGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCAAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCg                                                                                                                     <  2:452314/140‑1 (MQ=255)

                   aTGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGt                                                                                                                    >  2:503044/1‑140 (MQ=255)

                        gTGCGACCACTGCCCATCATCAGTCACCAGCCCCAGCCCGGTGGTATAAATACCCACCCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               <  2:910237/140‑1 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  2:515100/1‑140 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  1:1097705/1‑140 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  2:1170234/1‑140 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  2:549718/1‑140 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  2:841230/1‑140 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               <  1:549718/140‑1 (MQ=255)

                        gTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGc                                                                                                               >  1:1011020/1‑140 (MQ=255)

                         tGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCa                                                                                                              >  2:91109/1‑140 (MQ=255)

                         tGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCa                                                                                                              >  2:1415810/1‑140 (MQ=255)

                         tGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCa                                                                                                              >  1:1099020/1‑140 (MQ=255)

                          gCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCaa                                                                                                             >  1:1179905/1‑140 (MQ=255)

                           cGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCaaa                                                                                                            >  2:1013338/1‑140 (MQ=255)

                           cGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCaaa                                                                                                            >  2:40775/1‑140 (MQ=255)

                           cGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCaaa                                                                                                            >  1:643289/1‑140 (MQ=255)

                             cccccTGCCCATCATCAGTCATCAGCCCCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACa                                                                                                          <  2:1241832/136‑1 (MQ=255)

                             cccACTGACCATCATCAGTCATCAGCACCAGCCCGGTGGTATCAATACCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACa                                                                                                          <  2:660433/139‑1 (MQ=255)

                                  tGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGcc                                                                                                     <  1:1215070/140‑1 (MQ=255)

                                    cccATCATCGGTCATCAGCCCCAGCCCGGTGGTACCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCt                                                                                                   <  2:1348733/139‑1 (MQ=255)

                                    aCCATCATCAGTCATCAGCCCCAGACCGGTGGTATAAATATCAAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCt                                                                                                   <  2:687666/140‑1 (MQ=255)

                                    aCCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCt                                                                                                   <  1:1415810/140‑1 (MQ=255)

                                         catcagtcatcagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGc                                                                                              >  1:164366/1‑140 (MQ=255)

                                              gtcatcagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTAtt                                                                                         <  2:1184202/140‑1 (MQ=255)

                                              gtcatcagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTAtt                                                                                         <  1:1170234/140‑1 (MQ=255)

                                                 atcagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAg                                                                                      >  1:1495014/1‑140 (MQ=255)

                                                  tcagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGc                                                                                     <  1:841230/140‑1 (MQ=255)

                                                  ccagCCCCAGCCGGGTGGTATAAATACCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGc                                                                                     <  2:1442156/139‑1 (MQ=255)

                                                   cagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCa                                                                                    >  2:1453940/1‑140 (MQ=255)

                                                   cagCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCa                                                                                    <  1:515100/140‑1 (MQ=255)

                                                     gCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATg                                                                                  <  2:575508/140‑1 (MQ=255)

                                                      cACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGa                                                                                 >  1:1138672/1‑140 (MQ=255)

                                                      cACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGa                                                                                 >  1:1368092/1‑140 (MQ=255)

                                                       aCCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGaa                                                                                <  1:40775/140‑1 (MQ=255)

                                                         cAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGt                                                                              >  2:35321/1‑140 (MQ=255)

                                                           gACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCa                                                                                    <  1:1314782/132‑1 (MQ=255)

                                                           gACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCa                                                                                    >  2:1314782/1‑132 (MQ=255)

                                                               ggtggtATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTg                                                                        <  1:1013712/140‑1 (MQ=255)

                                                                gtggtATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTgg                                                                       >  1:1006134/1‑140 (MQ=255)

                                                                gtggtATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTgg                                                                       >  2:423757/1‑140 (MQ=255)

                                                                          tatCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTtgc                                                             >  2:1458769/1‑140 (MQ=255)

                                                                             ccAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTtgctgc                                                          >  2:249236/1‑140 (MQ=255)

                                                                              cAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTgcg                                                         >  1:1015435/1‑140 (MQ=255)

                                                                              cAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTgcg                                                         >  2:650053/1‑140 (MQ=255)

                                                                               aaCCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTgcgc                                                        >  1:107440/1‑140 (MQ=255)

                                                                                accgcaccgcCTCGCGCAGTTTCCACGCTACTGGTTCATCAACAAAATAGAGCACCGCTGGGCGATCAGGGTCGACCGAGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGcc                                                       >  2:211717/1‑140 (MQ=255)

                                                                                aCCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGcc                                                       >  1:595550/1‑140 (MQ=255)

                                                                                aCCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGcc                                                       >  1:85687/1‑140 (MQ=255)

                                                                                aCCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGcc                                                       >  2:684488/1‑140 (MQ=255)

                                                                                    ccccgccGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCg                                                   >  2:1170041/1‑140 (MQ=255)

                                                                                    ccccgccGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCg                                                   >  2:414261/1‑140 (MQ=255)

                                                                                    ccccgccGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCg                                                   >  2:1504352/1‑140 (MQ=255)

                                                                                    ccccgccGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCg                                                   <  1:1506573/140‑1 (MQ=255)

                                                                                    ccccgccGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCg                                                   >  2:1239950/1‑140 (MQ=255)

                                                                                          cgcATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGc                                             <  1:91109/140‑1 (MQ=255)

                                                                                          cgcATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGc                                             <  1:1453940/140‑1 (MQ=255)

                                                                                            cATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCa                                           <  2:1011020/140‑1 (MQ=255)

                                                                                                cAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGt                                       <  2:1145468/140‑1 (MQ=255)

                                                                                                     tCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCg                                  <  1:650053/140‑1 (MQ=255)

                                                                                                     tCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCg                                  <  1:310040/140‑1 (MQ=255)

                                                                                                      ccACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGc                                             <  1:1055197/128‑1 (MQ=255)

                                                                                                      ccACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGc                                             >  2:1055197/1‑128 (MQ=255)

                                                                                                      ccACGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGa                                 >  2:41821/1‑140 (MQ=255)

                                                                                                        aCGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAca                               <  1:35321/140‑1 (MQ=255)

                                                                                                        aCGCTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAca                               <  2:1138672/140‑1 (MQ=255)

                                                                                                           cTACCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcatca                            >  2:1345084/1‑140 (MQ=255)

                                                                                                             aCCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              <  1:444307/136‑1 (MQ=255)

                                                                                                             aCCGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              >  2:444307/1‑136 (MQ=255)

                                                                                                               cGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              <  2:1059271/134‑1 (MQ=255)

                                                                                                               cGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              >  1:1059271/1‑134 (MQ=255)

                                                                                                               cGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGtt                        >  2:614086/1‑140 (MQ=255)

                                                                                                               cGGTTCATCAAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGtt                        >  2:1325947/1‑140 (MQ=255)

                                                                                                                     atcaAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              <  1:1376267/128‑1 (MQ=255)

                                                                                                                     atcaAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGAcat                              >  2:1376267/1‑128 (MQ=255)

                                                                                                                     atcaAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGaa                  <  2:595550/140‑1 (MQ=255)

                                                                                                                      tcaAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAg                 >  1:1473995/1‑140 (MQ=255)

                                                                                                                       caAGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGc                <  2:1015435/140‑1 (MQ=255)

                                                                                                                        aaGAAAATAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCa               >  1:1487276/1‑140 (MQ=255)

                                                                                                                           aaaaTAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGtt            >  2:30732/1‑140 (MQ=255)

                                                                                                                            aaaTAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGttt           >  2:126311/1‑140 (MQ=255)

                                                                                                                               tAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGaa        <  2:1487276/140‑1 (MQ=255)

                                                                                                                               tAGAGCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGaa        <  2:1368092/140‑1 (MQ=255)

                                                                                                                                 gagCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACg      <  2:1179905/140‑1 (MQ=255)

                                                                                                                                  agCACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGc     >  1:8907/1‑140 (MQ=255)

                                                                                                                                    cACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTg   >  1:99383/1‑140 (MQ=255)

                                                                                                                                    cACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTg   >  1:1284162/1‑140 (MQ=255)

                                                                                                                                    cACCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTg   <  2:1097705/140‑1 (MQ=255)

                                                                                                                                     aCCGCTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGc  <  1:684488/140‑1 (MQ=255)

ATGATGGCGCGGAGAAGTAATGCGGTGCGACCACTGACCATCATCAGTCATCAGCACCAGACCGGTGGTATCAATATCCAACCGCCCCGCCGCATGCAGTTTCCACGCTACCGGTTCATCAAGAAAATAGAGCACCGTTGGGTGATCAGGGTCGTCCGTGGAGCAAACATAGCCCTGAGGCTTATTGAGCATGAAGTAACGTGGACCGTGTTGCTGCGCCAGCGGGTTGCCATCGTAAGCGACATCATGTTCAGGAAGCAGTTTGAACGCTGC  >  NC_000913/2280103‑2280375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`