Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,319,449	T→C	N143S (AAC→AGC)	rcsC ←	sensory histidine kinase RcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,319,449	0	T	C	100.0%	177.6 / NA	50	N143S (AAC→AGC)	rcsC	sensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

GTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAG  >  NC_000913/2319321‑2319578                                                                                                                                 |                                                                                                                                  gtggtgCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTaa                                                                                                                        >  2:1295032/1‑140 (MQ=255)            cGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGaaaa                                                                                                             >  2:393671/1‑140 (MQ=255)                 tttattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCTAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTAt                                                                                                        <  2:531601/140‑1 (MQ=255)                 tttattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTAt                                                                                                        >  1:943181/1‑140 (MQ=255)                 tttattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTAt                                                                                                        <  1:1315195/140‑1 (MQ=255)                 tttattGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTAt                                                                                                        <  2:392762/140‑1 (MQ=255)                          gCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGt                                                                                               <  1:393671/140‑1 (MQ=255)                          gCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGt                                                                                               <  1:1507206/140‑1 (MQ=255)                             ttCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAgc                                                                                            >  2:233081/1‑140 (MQ=255)                               cATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAgcgc                                                                                          <  1:233081/140‑1 (MQ=255)                                      aGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAAcc                                                                                   >  1:388911/1‑140 (MQ=255)                                        cTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTa                                                                                              >  2:916404/1‑127 (MQ=255)                                        cTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTa                                                                                              <  1:916404/127‑1 (MQ=255)                                          ttCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGc                                                                               >  2:676066/1‑140 (MQ=255)                                              aCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt                                                                           <  1:834664/140‑1 (MQ=255)                                              aCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt                                                                           <  1:676066/140‑1 (MQ=255)                                              aCGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt                                                                           <  2:943181/140‑1 (MQ=255)                                                  ggTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt                                                                           <  2:106872/136‑1 (MQ=255)                                                  ggTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCACTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAAt                                                                           >  1:106872/1‑136 (MQ=255)                                                           ttCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAAc                                                              >  1:191205/1‑140 (MQ=255)                                                              cACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTc                                                           <  1:1295032/140‑1 (MQ=255)                                                              cACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTc                                                           >  2:715149/1‑140 (MQ=255)                                                              cACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTc                                                           >  1:237162/1‑140 (MQ=255)                                                              cACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTc                                                           >  2:1427303/1‑140 (MQ=255)                                                                 tGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGcc                                                        <  2:388911/140‑1 (MQ=255)                                                                 tGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGcc                                                        <  1:715149/140‑1 (MQ=255)                                                                 tGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGcc                                                        <  1:1427303/140‑1 (MQ=255)                                                                   gCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAg                                                      <  1:759755/140‑1 (MQ=255)                                                                    cATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAgg                                                     >  2:539852/1‑140 (MQ=255)                                                                      tatCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGgtg                                                   >  2:102246/1‑140 (MQ=255)                                                                         cACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTa                                                >  1:1409503/1‑140 (MQ=255)                                                                                aCCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTg                                         <  1:113895/140‑1 (MQ=255)                                                                                 ccGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGc                                        >  2:266266/1‑140 (MQ=255)                                                                                 ccGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGc                                        <  1:511035/140‑1 (MQ=255)                                                                                  cGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACt                                              <  2:1409165/133‑1 (MQ=255)                                                                                  cGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACt                                              >  1:1409165/1‑133 (MQ=255)                                                                                  cGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGATGTTACTCATTGCg                                       >  2:932975/1‑140 (MQ=255)                                                                                   gAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCgg                                      <  2:191205/140‑1 (MQ=255)                                                                                   gAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCgg                                      <  1:298254/140‑1 (MQ=255)                                                                                     aaTTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGaa                                    <  1:102246/140‑1 (MQ=255)                                                                                     aaTTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGaa                                    <  1:266266/140‑1 (MQ=255)                                                                                          gCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATc                               <  1:932975/140‑1 (MQ=255)                                                                                          gCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATc                               >  2:42165/1‑140 (MQ=255)                                                                                            cATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCgg                             <  1:539852/140‑1 (MQ=255)                                                                                            cATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCgg                             >  2:1219814/1‑140 (MQ=255)                                                                                                     gTTATCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCaa                    >  2:1215624/1‑140 (MQ=255)                                                                                                        aTCGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACa                 >  2:305764/1‑140 (MQ=255)                                                                                                          cGCTGCCGATTAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGc               >  2:783887/1‑140 (MQ=255)                                                                                                                    tAAAAATACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACg     >  2:1510742/1‑140 (MQ=255)                                                                                                                       aaaTACCCGGCTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAg  <  1:1219814/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  GTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCGCAGAAAAATTATCGCGCCAGTAGCGCATAAACCACGCCAATGACTCCAGAGAACCTCGCCAGGTGTTACTCATTGCGGAACAATCGGAGTCGGCAAACAGCGGTTCAAACGCAG  >  NC_000913/2319321‑2319578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑