Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	98.4%	206.3 / ‑5.9	61	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (36/24);  minor base A (0/1);  total (36/25)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAG  >  NC_000913/2446442‑2446714                                                                                                                                          |                                                                                                                                        cAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCaa                                                                                                                                       <  1:552555/140‑1 (MQ=255)      gATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTg                                                                                                                                  >  1:1348273/1‑140 (MQ=255)        tCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGa                                                                                                                                >  1:450030/1‑140 (MQ=255)                 aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                       <  2:450030/140‑1 (MQ=255)                  cGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTc                                                                                                                      <  2:736249/140‑1 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                    >  1:77118/1‑140 (MQ=255)                    aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                    <  2:1295157/140‑1 (MQ=255)                      cGCCAGCATCGCTTCTGCGATCTGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCa                                                                                                                     >  1:357601/1‑137 (MQ=255)                      cGCCAGCATCGCTTCTGCGATCTGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCa                                                                                                                     <  2:357601/137‑1 (MQ=255)                          aGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCat                                                                                                              <  2:1278490/140‑1 (MQ=255)                               cGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                         <  2:1477881/140‑1 (MQ=255)                                      gCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAt                                                                                                  <  2:1348273/140‑1 (MQ=255)                                      gCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAt                                                                                                  >  1:1240498/1‑140 (MQ=255)                                         aTCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtg                                                                                               >  1:63083/1‑140 (MQ=255)                                            ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                            >  1:953071/1‑140 (MQ=255)                                            ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                            >  1:436783/1‑140 (MQ=255)                                            ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                            >  1:450263/1‑140 (MQ=255)                                             gCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctgc                                                                                           <  2:1240498/140‑1 (MQ=255)                                              cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                          <  2:928289/140‑1 (MQ=255)                                              cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                          <  2:600626/140‑1 (MQ=255)                                              cACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGcc                                                                                          <  2:63083/140‑1 (MQ=255)                                                 tGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgc                                                                                       >  2:638584/1‑140 (MQ=255)                                                   cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                     >  1:1424770/1‑140 (MQ=255)                                                        aTCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGAt                                                                                >  2:802739/1‑140 (MQ=255)                                                                acaGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCCGTTAATGGTCCGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCga                                                                        <  2:1357763/140‑1 (MQ=255)                                                                  aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                      >  2:1119601/1‑140 (MQ=255)                                                                  aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                      >  2:1241033/1‑140 (MQ=255)                                                                    ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                    >  2:1290698/1‑140 (MQ=255)                                                                    ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                    >  1:524457/1‑140 (MQ=255)                                                                          tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                              >  2:814797/1‑140 (MQ=255)                                                                            aCGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGccgcccg                                                            >  2:418914/1‑140 (MQ=255)                                                                              ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgc                                                                  >  1:967562/1‑132 (MQ=255)                                                                              ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                          >  2:1155185/1‑140 (MQ=255)                                                                              ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGAAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgc                                                                  <  2:967562/132‑1 (MQ=255)                                                                                ccTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATg                                                        >  1:1285845/1‑140 (MQ=255)                                                                                ccTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATg                                                        >  2:777310/1‑140 (MQ=255)                                                                                 cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                       <  1:1155185/140‑1 (MQ=255)                                                                                   ttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGtt                                                     <  1:638584/140‑1 (MQ=255)                                                                                     ggTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGc                                                   >  1:370761/1‑140 (MQ=255)                                                                                            atcTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaa                                            <  2:1424770/140‑1 (MQ=255)                                                                                              ctcAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                          >  1:391770/1‑140 (MQ=255)                                                                                                      cttcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTg                                  >  1:502755/1‑140 (MQ=255)                                                                                                        tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                                >  2:271212/1‑140 (MQ=255)                                                                                                        tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                                >  1:346120/1‑140 (MQ=255)                                                                                                        tcGCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                                >  2:657847/1‑140 (MQ=255)                                                                                                          gCCAAAGCGGTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGa                              <  1:418914/140‑1 (MQ=255)                                                                                                                  ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                      <  1:271212/140‑1 (MQ=255)                                                                                                                  ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                      <  2:953071/140‑1 (MQ=255)                                                                                                                  ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                      <  2:436783/140‑1 (MQ=255)                                                                                                                  ggTTAATGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                      <  2:450263/140‑1 (MQ=255)                                                                                                                       ttGGTACGACCCGGCAGGGCAAGGCTGGGGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGtt                 <  2:1091156/139‑1 (MQ=255)                                                                                                                        tGGTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTc                >  2:914060/1‑140 (MQ=255)                                                                                                                          gTACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTg              >  2:875036/1‑140 (MQ=255)                                                                                                                           tACGACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTgg             >  1:22395/1‑140 (MQ=255)                                                                                                                              gACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg          >  2:655728/1‑140 (MQ=255)                                                                                                                              gACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg          >  2:506815/1‑140 (MQ=255)                                                                                                                              gACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTAGGTGATTTCATCGCGGTTCTGGCTg          >  1:429868/1‑140 (MQ=255)                                                                                                                                cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGcc        >  1:893115/1‑140 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc      <  2:1285845/140‑1 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc      >  2:625159/1‑140 (MQ=255)                                                                                                                                  cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc      >  2:433206/1‑140 (MQ=255)                                                                                                                                   ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg     <  1:625159/140‑1 (MQ=255)                                                                                                                                     cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa   <  1:655728/140‑1 (MQ=255)                                                                                                                                     cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa   >  1:1309041/1‑140 (MQ=255)                                                                                                                                      aCGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAg  <  2:1090600/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CAGGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACGACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCAG  >  NC_000913/2446442‑2446714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑