Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,447,800	T→C	E214G (GAA→GGA)	prmB ←	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,447,800	0	T	C	100.0%	171.0 / NA	50	E214G (GAA→GGA)	prmB	50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/36);  total (14/36)

TCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCA  >  NC_000913/2447666‑2447928                                                                                                                                       |                                                                                                                                  tCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCa                                                                                                                              <  1:49533/140‑1 (MQ=255) tCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCa                                                                                                                              <  1:421203/140‑1 (MQ=255)  cAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCAt                                                                                                                             <  1:321856/140‑1 (MQ=255)   aaCACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATc                                                                                                                            >  1:461258/1‑140 (MQ=255)     cacGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGa                                                                                                                          >  1:1339524/1‑140 (MQ=255)     cacGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGa                                                                                                                          <  2:461258/140‑1 (MQ=255)      acGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGAc                                                                                                                         <  1:990823/140‑1 (MQ=255)       cGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACa                                                                                                                        <  1:996297/140‑1 (MQ=255)          catcatCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCCGGGCGGACATATCTCCCGCATCGACATAc                                                                                                                     >  2:1067333/1‑140 (MQ=255)             catcaGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATAcggc                                                                                                                  <  1:1111937/140‑1 (MQ=255)             catcaGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATAcggc                                                                                                                  <  1:241626/140‑1 (MQ=255)                 aGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGt                                                                                                              <  1:339682/140‑1 (MQ=255)                 aGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGt                                                                                                              <  1:1429851/140‑1 (MQ=255)                 aGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGt                                                                                                              <  2:829620/140‑1 (MQ=255)                 aGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGt                                                                                                              <  1:1271125/140‑1 (MQ=255)                    aaGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCggggttg                                                                                                           >  2:779766/1‑136 (MQ=255)                    aaGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGGATTCGTTTGGCAGGGCCGGCATATCTCCCCCATCGACATACCGGGGGGTaa                                                                                                           >  2:980804/1‑139 (MQ=255)                     aGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGt                                                                                                          <  1:1261992/140‑1 (MQ=255)                      ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTg                                                                                                         >  2:1172774/1‑140 (MQ=255)                      ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTg                                                                                                         >  1:908377/1‑140 (MQ=255)                      ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGGTtgtg                                                                                                         >  2:1257146/1‑140 (MQ=255)                      ggTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGGTtgtg                                                                                                         >  1:406210/1‑140 (MQ=255)                        tAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGGTAGTgga                                                                                                       >  1:1359652/1‑138 (MQ=255)                          aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACaa                                                                                                     <  2:908377/140‑1 (MQ=255)                          aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACaa                                                                                                     <  1:83171/140‑1 (MQ=255)                          aTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACaa                                                                                                     <  1:329776/140‑1 (MQ=255)                                   gTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGt                                                                                            <  1:1435059/140‑1 (MQ=255)                                                 gACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGGTTGGGGCAAACAAGGCGGACTGCACCTTTCgc                                                                              >  1:953662/1‑139 (MQ=255)                                                  aCGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCa                                                                            <  2:93720/140‑1 (MQ=255)                                                  aCGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCa                                                                            <  2:195245/140‑1 (MQ=255)                                                     cgTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGGCGGACATATCTCCCGCATCGACATACGGCGGGGTTGTGACAAACAAGGCGGGCTTCACCTTTCGGCAAg                                                                          >  2:590146/1‑140 (MQ=255)                                                       tCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTcg                                                                       <  1:639565/140‑1 (MQ=255)                                                       tCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTcg                                                                       <  1:394296/140‑1 (MQ=255)                                                            ttCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGaa                                                                  <  1:779688/140‑1 (MQ=255)                                                                ggCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGa                                                              <  1:322412/140‑1 (MQ=255)                                                                ggCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGGCGGGCATATCTCCCGCCTCGACATACGGCGGGGTTGTGACAAACAAGGCGTTCTGCA‑CTTTTTGGCAGTCGCgggaaaaa                                                              >  2:322412/1‑134 (MQ=255)                                                                     tCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGGTAGGGGCAAACAAGGCGGACTTCA‑CCTTTCGCAAGGCGCGGGACAAAACggg                                                         >  1:510113/1‑139 (MQ=255)                                                                               aTGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGa                                               <  2:895428/140‑1 (MQ=255)                                                                                tGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACAGATCTCCCGCATCGACATGCGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGaa                                              <  1:859692/140‑1 (MQ=255)                                                                                  ccAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATg                                            <  2:20398/140‑1 (MQ=255)                                                                                   cAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGa                                           <  2:941105/140‑1 (MQ=255)                                                                                     ggCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACg                                         <  2:26735/140‑1 (MQ=255)                                                                                       cccAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGtt                                       <  1:1180284/140‑1 (MQ=255)                                                                                             tCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGAt                                 <  2:274264/140‑1 (MQ=255)                                                                                              ccGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATc                                <  1:1172774/140‑1 (MQ=255)                                                                                                        gCGGTATTCGTTTGGCAGGTCGGACATATCTCCCGCATCAACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGtt                      <  1:1452342/140‑1 (MQ=255)                                                                                                                cGTTTGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtg              <  1:1495735/140‑1 (MQ=255)                                                                                                                    tGGCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttct          <  2:510113/140‑1 (MQ=255)                                                                                                                     ggCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttctg         <  1:340254/140‑1 (MQ=255)                                                                                                                      gCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGGCAATCAGGGCGGACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGGATCGGGATGACGGTGTGGATCAAGCCGGGTTTTTCGAtgttcttt        >  2:1001946/1‑138 (MQ=255)                                                                                                                      gCAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttctgt        >  2:1073719/1‑140 (MQ=255)                                                                                                                       cAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttctgtt       >  1:1421520/1‑140 (MQ=255)                                                                                                                       cAGGTCGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCACGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGAtgttctgtt       <  2:1359652/140‑1 (MQ=255)                                                                                                                            cGGACATATCTCCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCa  >  1:21071/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  TCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGTTTGGCAGGTCGGACATATCTTCCGCATCGACATACGGCGGGTTAGTGACAATCAGGTCGTACTGCA‑CTTTCGGCAAGTCGCGGAACAGATCGGAACGAATCGGAATGACGTTGTGGATCAGACCGTGTTCTTCGATGTTCTGTTCAGCA  >  NC_000913/2447666‑2447928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑