Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,606,033	A→G	E408G (GAG→GGG)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,606,033	0	A	G	100.0%	190.3 / NA	55	E408G (GAG→GGG)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/21);  total (34/21)

CGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGCTGCCA  >  NC_000913/2605902‑2606169                                                                                                                                    |                                                                                                                                         cGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGa                                                                                                                                  >  2:659803/1‑140 (MQ=255)   tATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATc                                                                                                                                >  2:1298088/1‑140 (MQ=255)           acCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTAc                                                                                                                                      <  2:234141/126‑1 (MQ=255)           acCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTAc                                                                                                                                      >  1:234141/1‑126 (MQ=255)           acCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGcc                                                                                                                        >  2:929934/1‑140 (MQ=255)             ccTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCg                                                                                                                      >  2:782701/1‑140 (MQ=255)             ccTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCg                                                                                                                      >  1:526969/1‑140 (MQ=255)             ccTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCg                                                                                                                      >  1:388226/1‑140 (MQ=255)              cTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGc                                                                                                                     <  2:257636/140‑1 (MQ=255)               tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGcc                                                                                                                    >  1:1284441/1‑140 (MQ=255)               tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGGGGGGTACTGGATCCTGCTGCCCGcc                                                                                                                    >  2:235166/1‑140 (MQ=255)               tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAACGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGcc                                                                                                                    >  1:171571/1‑140 (MQ=255)                gCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCa                                                                                                                   >  2:174107/1‑140 (MQ=255)                  cGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATg                                                                                                                 >  2:333596/1‑140 (MQ=255)                   gTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGa                                                                                                                <  1:428410/140‑1 (MQ=255)                     tGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGAtt                                                                                                              <  1:997273/140‑1 (MQ=255)                     tGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGAtt                                                                                                              <  1:333596/140‑1 (MQ=255)                          ggCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATtcttct                                                                                                         <  1:155169/140‑1 (MQ=255)                          ggCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATtcttct                                                                                                         >  2:455328/1‑140 (MQ=255)                           gCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCtg                                                                                                        <  1:1298088/140‑1 (MQ=255)                              tGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCtgatg                                                                                                     <  2:526969/140‑1 (MQ=255)                                ggTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTgatgat                                                                                                   >  2:513150/1‑140 (MQ=255)                                      tGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATc                                                                                             >  1:918944/1‑140 (MQ=255)                                                cgcTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGt                                                                                   >  1:952222/1‑140 (MQ=255)                                                cgcTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGt                                                                                   <  1:782701/140‑1 (MQ=255)                                                  cTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGttt                                                                                 >  1:1404178/1‑140 (MQ=255)                                                      ggATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGcc                                                                             <  2:628424/139‑1 (MQ=255)                                                          tACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGt                                                                         >  2:626204/1‑140 (MQ=255)                                                               gCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTAtt                                                                    >  2:1350214/1‑140 (MQ=255)                                                                 gTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCg                                                                  >  2:122948/1‑140 (MQ=255)                                                                   gccgccGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCt                                                                >  2:759955/1‑140 (MQ=255)                                                                    ccgccgTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTAtt                                                                    >  1:1339294/1‑135 (MQ=255)                                                                    ccgccgTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTAtt                                                                    <  2:1339294/135‑1 (MQ=255)                                                                    ccgccgTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTg                                                               <  1:174107/140‑1 (MQ=255)                                                                          ttCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTgg                                                         >  2:56492/1‑140 (MQ=255)                                                                          ttCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTgg                                                         <  1:455328/140‑1 (MQ=255)                                                                               cGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGTTTTTGCGTTGTCGGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTcgcg                                                    <  2:171571/140‑1 (MQ=255)                                                                               cGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTcgcg                                                    <  1:1043422/140‑1 (MQ=255)                                                                               cGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTcgcg                                                    <  1:396653/140‑1 (MQ=255)                                                                                   ttcttcAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTtgt                                                <  2:576126/140‑1 (MQ=255)                                                                                             aaTTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCaaa                                      <  1:759955/140‑1 (MQ=255)                                                                                              aTTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAAc                                     >  1:1500674/1‑140 (MQ=255)                                                                                                  ccGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACcgag                                 >  1:138751/1‑140 (MQ=255)                                                                                                     cTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACcgagcga                              >  1:824324/1‑140 (MQ=255)                                                                                                       gTTTGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAgg                            <  1:235166/140‑1 (MQ=255)                                                                                                        tttGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGc                           >  2:1037466/1‑140 (MQ=255)                                                                                                         ttGCTGCCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGcc                          >  2:727970/1‑140 (MQ=255)                                                                                                          tgctgcCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGTAAACCGAGCGAGGCCg                         >  1:72030/1‑140 (MQ=255)                                                                                                              gcCGGTTTTGCGTTGTCAGTGGGGTGCGGGTTCGTGCTCCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGTAAACCGAGCGAGGCCGTCGc                     <  2:72030/140‑1 (MQ=255)                                                                                                              gcCGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTTGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGc                     >  2:1126991/1‑140 (MQ=255)                                                                                                                cGGTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCg                   <  1:727970/140‑1 (MQ=255)                                                                                                                 ggTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGa                  <  2:1489498/140‑1 (MQ=255)                                                                                                                  gTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGAt                 >  2:664622/1‑140 (MQ=255)                                                                                                                  gTTTTGCGTTGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGAt                 >  2:1481899/1‑140 (MQ=255)                                                                                                                           tGTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCg        <  2:824324/140‑1 (MQ=255)                                                                                                                            gTCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGc       >  2:1511180/1‑140 (MQ=255)                                                                                                                             tCAGTGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGCt      >  2:393444/1‑140 (MQ=255)                                                                                                                                 tGGGGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGCTGCCa  <  1:1481899/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCGCCAGTTTCGCCTGGTTTATTCGCTGGTTTGGTCGCGTTGTGCCTGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGCTGCCA  >  NC_000913/2605902‑2606169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑