Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,230	Δ1 bp	coding (615/1539 nt)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,227	0	G	.	100.0%	305.9 / NA	71	coding (612/1539 nt)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/46);  total (25/46)

TTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGAT  >  NC_000913/2813092‑2813361                                                                                                                                         |                                                                                                                                       ttttatCAGCGATAATTACCCCTGGGGCTGGGTAATCTTCATCAACGTGCCGATTTGCGCGGCGGGGGCGGTGGATGA‑AATGCAAAATCTGCGCCGGCGGGGAAACCCCACCCCAAGGGGGCGGGTTGATGCCGCggggg                                                                                                                                    >  2:1216065/4‑139 (MQ=255)      tCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACCCTGCAATCTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCAc                                                                                                                              <  1:1102945/140‑1 (MQ=255)        ggCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActg                                                                                                                            <  1:883323/139‑1 (MQ=255)        aGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActg                                                                                                                            <  1:582747/140‑1 (MQ=255)        aGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActg                                                                                                                            <  1:1132783/140‑1 (MQ=255)         gCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgc                                                                                                                           <  1:1285468/140‑1 (MQ=255)          cGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgct                                                                                                                          >  2:476939/1‑140 (MQ=255)          cGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgct                                                                                                                          >  2:436669/1‑140 (MQ=255)          cGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgct                                                                                                                          >  2:921810/1‑140 (MQ=255)           gATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTTATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgctg                                                                                                                         >  1:1061603/1‑140 (MQ=255)           gATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑GGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCActgctg                                                                                                                         >  2:605544/1‑140 (MQ=255)                   ccACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTgg                                                                                                                 <  2:214882/140‑1 (MQ=255)                    cACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGGTTGATGCCG‑GGGGCTGGGACTGCTGGGTATTggg                                                                                                                >  2:883323/1‑139 (MQ=255)                      cTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTAt                                                                                                              >  1:1229196/1‑140 (MQ=255)                        ggggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCg                                                                                                            >  1:1286656/1‑140 (MQ=255)                         gggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCgg                                                                                                           >  1:952703/1‑140 (MQ=255)                         gggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCgg                                                                                                           >  1:83257/1‑140 (MQ=255)                         gggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCgg                                                                                                           >  1:801786/1‑140 (MQ=255)                         gggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑GGGTGAAGACACGGCAAACTCTGCGCGGACGTGGAAACCCCACCGGACGGCGGGGGAGTTTTGCCG‑GGGGCCGGCACGGCTGGTTATTGGGATCgg                                                                                                           >  2:1019354/1‑140 (MQ=255)                          ggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGGTTTATGCCGTGGGG‑TGGCACTGCTGGTTATTGGGATCGGc                                                                                                          >  2:231320/1‑140 (MQ=255)                          ggCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGAGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGc                                                                                                          <  2:1229196/140‑1 (MQ=255)                               gATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCt                                                                                                     <  1:451325/140‑1 (MQ=255)                               gATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCt                                                                                                     >  1:603557/1‑140 (MQ=255)                                atatTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTg                                                                                                    <  1:476939/140‑1 (MQ=255)                                 tatTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGc                                                                                                   >  2:888081/1‑140 (MQ=255)                                  atTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCa                                                                                                  <  1:174039/140‑1 (MQ=255)                                    tcttcATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGa                                                                                                >  2:845165/1‑140 (MQ=255)                                      ttcATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGAtt                                                                                              <  2:766310/140‑1 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑TGTTGTTGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGGACGGGGGGGGGGTGTTGCCGT‑GGGCTGGCGCTGCTGGGTATTGGTATCGGCAGCCTGCAGAttttt                                                                                           >  2:453810/1‑137 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTg                                                                                                    >  1:726226/1‑131 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTg                                                                                                    <  2:726226/131‑1 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGGTATTGGTATCGGCAGCCTGCAGATTATg                                                                                           >  2:497110/1‑140 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑GGTTGGTGACACTGCAAACTCTGCGCGGGCGGGAAAACCGCACCCAACGGCGGCGGGTTTATGCCG‑GGGGGTGGGACTGCTGGGTATTTGGATCGGGCGCCTGCCGATTATg                                                                                           >  2:663147/1‑140 (MQ=255)                                         atcaACGTGCCGATTGGCGTGGCGGTGG‑GGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATg                                                                                           >  2:85335/1‑140 (MQ=255)                                              cGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGa                                                                                      <  1:1281058/140‑1 (MQ=255)                                              cGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGa                                                                                      <  1:1216065/140‑1 (MQ=255)                                              cGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGa                                                                                      <  1:231320/140‑1 (MQ=255)                                              cGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATACTCGa                                                                                      >  2:244998/1‑140 (MQ=255)                                                tGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGAcc                                                                                    >  1:812771/1‑140 (MQ=255)                                                tGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGAcc                                                                                    >  1:916225/1‑140 (MQ=255)                                                 gCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACcg                                                                                   <  2:812771/140‑1 (MQ=255)                                                 gCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACcg                                                                                   <  2:104807/140‑1 (MQ=255)                                                     aTTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGGTTGATGCCGT‑GGGCTGGCACTGCTGGTTTTTGGTATCGGCAGCCTGTAGATTATGCTCGACCGCggg                                                                               >  2:578386/1‑139 (MQ=255)                                                      ttGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTa                                                                              <  2:801786/140‑1 (MQ=255)                                                      ttGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTa                                                                              <  1:921810/140‑1 (MQ=255)                                                      ttGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTa                                                                              >  2:756980/1‑140 (MQ=255)                                                          cgtggcgGAGG‑GGTTGGTGACACTGCCAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGGGTGATGGCGTGGGG‑TGGGCCTGCCGGGTATTGGGATCGGGAGCCTGCAGATTATGCTCGACCGCGGGTAaga                                                                          >  2:1322072/1‑140 (MQ=255)                                                                ggtgg‑tGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGa                                                                    <  1:845165/140‑1 (MQ=255)                                                                   gg‑tgTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGGTTGATGCCGTGGGG‑TGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTg                                                                 >  2:554167/1‑140 (MQ=255)                                                                   gg‑tgTTGATGACACTGCAAAATCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTg                                                                 <  2:827529/140‑1 (MQ=255)                                                                       gtTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGtt                                                              <  1:436669/140‑1 (MQ=255)                                                                        ttGATGACACTGCAAAATCTGCGCGGACGGGAAACCCGCACCGAACGGCGGCGGGTTGATGCCGTGGGG‑TGGCACTGCTGGTTTTTGGTATCGGCAGCCTGCAGATTTTGCTCGACCGCGGGAAAGAGCTGGGCTGGttt                                                             >  2:527165/1‑140 (MQ=255)                                                                          gatgaCACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTtc                                                           >  1:583155/1‑140 (MQ=255)                                                                              acacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTtcatca                                                       <  1:85335/140‑1 (MQ=255)                                                                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGa                                                                    >  2:327142/1‑126 (MQ=255)                                                                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGa                                                                    <  1:327142/126‑1 (MQ=255)                                                                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATcac                                                      >  2:486357/1‑140 (MQ=255)                                                                               cacTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATcac                                                      >  2:229651/1‑140 (MQ=255)                                                                                acTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCAT‑GGGCTGGCACTGCTGGTTATTGGAATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATcaca                                                     >  2:1358658/1‑140 (MQ=255)                                                                                  tGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAgg                                                   >  2:140481/1‑140 (MQ=255)                                                                                   gCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGa                                                  <  1:1036076/140‑1 (MQ=255)                                                                                   gCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGa                                                  <  1:1019354/140‑1 (MQ=255)                                                                                     aaaCTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGTCCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGaaa                                                >  2:739167/1‑140 (MQ=255)                                                                                        ctctGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTa                                             <  1:888081/140‑1 (MQ=255)                                                                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                         >  2:184558/1‑140 (MQ=255)                                                                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                         <  2:83257/140‑1 (MQ=255)                                                                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                         <  1:140481/140‑1 (MQ=255)                                                                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                         >  2:248956/1‑140 (MQ=255)                                                                                            gcgcGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATACTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTatcat                                         <  1:244998/140‑1 (MQ=255)                                                                                              gcgGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGGAATTATCATcc                                       >  1:1157932/1‑140 (MQ=255)                                                                                              gcgGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTat                                            >  1:559679/1‑135 (MQ=255)                                                                                              gcgGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTat                                            <  2:559679/135‑1 (MQ=255)                                                                                              gcgGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATcc                                       >  2:113862/1‑140 (MQ=255)                                                                                                  aCGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTAc                                   <  2:603557/140‑1 (MQ=255)                                                                                                     tGAAACCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgt                                >  2:192240/1‑140 (MQ=255)                                                                                                         aCCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgtggtg                            <  1:184558/140‑1 (MQ=255)                                                                                                         aCCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgtggtg                            <  1:248956/140‑1 (MQ=255)                                                                                                         aCCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgtggtg                            <  2:333741/140‑1 (MQ=255)                                                                                                         aCCCGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCgtggtg                            <  1:229651/140‑1 (MQ=255)                                                                                                          cccGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGtggtgg                           <  2:1061603/140‑1 (MQ=255)                                                                                                            cGCAGCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCg                         <  2:1286656/140‑1 (MQ=255)                                                                                                            cGCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCg                         >  1:529037/1‑140 (MQ=255)                                                                                                             gCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCgg                        <  1:756980/140‑1 (MQ=255)                                                                                                             gCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCgg                        <  1:663147/140‑1 (MQ=255)                                                                                                             gCACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGCTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCgg                        <  1:497110/140‑1 (MQ=255)                                                                                                              cACCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCggt                       >  1:890768/1‑140 (MQ=255)                                                                                                               aCCGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCggtg                      <  1:486357/140‑1 (MQ=255)                                                                                                                ccGAACGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCggtgg                     <  2:358189/140‑1 (MQ=255)                                                                                                                   aaCGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGtggtgg                           <  1:859639/131‑1 (MQ=255)                                                                                                                   aaCGGCGGCGGATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGtggtgg                           >  2:859639/1‑131 (MQ=255)                                                                                                                        cggcggATTGATGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATc             <  1:274758/140‑1 (MQ=255)                                                                                                                                   tGCCGT‑GGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGAt  <  1:605544/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       TTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGG‑TGTTGATGACACTGCAAACTCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCGGCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGAT  >  NC_000913/2813092‑2813361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑