Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,550	(G)5→6	coding (635/1782 nt)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,545	1	.	G	98.0%	180.6 / ‑3.2	51	coding (630/1782 nt)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (25/25);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

TCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑GGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGG  >  NC_000913/534406‑534678                                                                                                                                             |                                                                                                                                       tCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGcc                                                                                                                                         <  1:563916/140‑1 (MQ=255)  cGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑g                                                                                                                                       >  2:333827/1‑140 (MQ=255)       ttCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCCGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑gggg‑gg                                                                                                                                 <  2:645408/140‑2 (MQ=255)           ggTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑gggg‑ggcggg                                                                                                                             <  1:116754/140‑6 (MQ=255)           ggTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑gggg‑ggcggg                                                                                                                             <  1:333827/140‑6 (MQ=255)           ggTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑gggg‑ggcggg                                                                                                                             <  1:683782/140‑6 (MQ=255)                         aGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGa                                                                                                                <  2:557429/140‑1 (MQ=255)                            ttGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGc                                                                                                             <  1:79465/140‑1 (MQ=255)                                 ccTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTg                                                                                                        <  2:698282/140‑1 (MQ=255)                                  cTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGc                                                                                                       <  1:11708/140‑1 (MQ=255)                                  cTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGc                                                                                                       <  2:710178/140‑1 (MQ=255)                                   tGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCa                                                                                                      <  2:1065865/140‑1 (MQ=255)                                    gACATGTACGAACCACTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGcgc                                                                                                     <  1:914560/140‑3 (MQ=255)                                         gTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTa                                                                                                >  2:1082209/1‑140 (MQ=255)                                          tACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACg                                                                                                              <  2:261937/125‑1 (MQ=255)                                          tACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACg                                                                                                              >  1:261937/1‑125 (MQ=255)                                          tACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTac                                                                                               >  2:514896/1‑140 (MQ=255)                                          tACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTac                                                                                               >  2:568025/1‑140 (MQ=255)                                                    tGCCGGTCTACAAACCTGCTGCCAGCCATATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTg                                                                                      <  1:93915/139‑1 (MQ=255)                                                    tGCCGGTCTACAAACCTGCTGCCAGCCATATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTg                                                                                      >  2:93915/1‑139 (MQ=255)                                                                aaCCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAg                                                                         >  1:1171894/1‑140 (MQ=255)                                                                    tgctgcCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGtt                                                                     >  2:217261/1‑140 (MQ=255)                                                                     gctgcCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTc                                                                    >  1:230406/1‑140 (MQ=255)                                                                      ctgcCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTcc                                                                   >  2:252507/1‑140 (MQ=255)                                                                             ccGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATc                                                            >  2:692531/1‑140 (MQ=255)                                                                                tATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCa                                                         >  1:745546/1‑140 (MQ=255)                                                                                       aTCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTaa                                                  >  2:960610/1‑140 (MQ=255)                                                                                       aTCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTaa                                                  >  2:1093388/1‑140 (MQ=255)                                                                                       aTCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTaa                                                  >  1:68766/1‑140 (MQ=255)                                                                                            aaaaGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATgggc                                             >  2:824144/1‑140 (MQ=255)                                                                                              aaGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATgggctg                                           <  2:1171894/140‑1 (MQ=255)                                                                                                      gAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTa                                   <  1:514896/140‑1 (MQ=255)                                                                                                         aTGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATcc                                >  1:1123373/1‑140 (MQ=255)                                                                                                           gTTAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCg                              >  1:40768/1‑140 (MQ=255)                                                                                                            ttAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCgg                             >  2:1038878/1‑140 (MQ=255)                                                                                                            ttAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCgg                             >  2:700399/1‑140 (MQ=255)                                                                                                             tAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGa                            <  2:234871/140‑1 (MQ=255)                                                                                                             tAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGa                            <  2:745546/140‑1 (MQ=255)                                                                                                             tAATCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGa                            <  1:252507/140‑1 (MQ=255)                                                                                                              aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATgggc                                             >  2:1387840/1‑122 (MQ=255)                                                                                                              aaTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATgggc                                             <  1:1387840/122‑1 (MQ=255)                                                                                                               aTCCAGGCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACg                          <  1:568025/140‑1 (MQ=255)                                                                                                                     gCCGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATg                    >  2:1059597/1‑140 (MQ=255)                                                                                                                      ccGAACGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGa                   >  2:237440/1‑140 (MQ=255)                                                                                                                         aaCGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGa                            >  1:1245516/1‑128 (MQ=255)                                                                                                                         aaCGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGa                            <  2:1245516/128‑1 (MQ=255)                                                                                                                          aCGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACt                <  2:1260884/139‑1 (MQ=255)                                                                                                                          aCGTCCGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACt                >  1:1260884/1‑139 (MQ=255)                                                                                                                               cGGTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGc          >  1:867057/1‑140 (MQ=255)                                                                                                                                ggTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGcc         <  2:1344182/140‑1 (MQ=255)                                                                                                                                ggTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGcc         <  2:1123373/140‑1 (MQ=255)                                                                                                                                ggTGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGcc         <  2:230406/140‑1 (MQ=255)                                                                                                                                  tGATTGTTGCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCgg       <  2:867057/140‑1 (MQ=255)                                                                                                                                     ttgttgCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGAt    <  1:1082209/140‑1 (MQ=255)                                                                                                                                       gttgCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGAtgg  <  1:700399/140‑1 (MQ=255)                                                                                                                                       gttgCCGGGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGAtgg  <  1:1038878/140‑1 (MQ=255)                                                                                                                                       gttgCC‑GGGGCGCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGAtgg  <  1:960610/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       TCGACGTTCAGGTTGCGGAAATCGAGTTTGATCCTGACATGTACGAACCGCTGCCGGTCTACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC‑GGGG‑GCGGGGTAATTAATGCTGACGCAGCTGCACTGTTACAACAGTTTGCTGAACTGACCAGCGTTCCGGTGATCCCAACGCTAATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGG  >  NC_000913/534406‑534678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑