Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,580	(G)7→8	noncoding (73/88 nt)	serX ←	tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,573	1	.	G	100.0%	46.6 / NA	15	noncoding (80/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (0/15);  total (0/15)

GTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCT  >  NC_000913/1097449‑1097707                                                                                                                               |                                                                                                                                       gTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGaa                                                                                                                          <  2:788415/140‑1 (MQ=14)   cTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAAcc                                                                                                                        >  1:234521/1‑140 (MQ=18)          aCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATa                                                                                                                 <  1:134362/140‑1 (MQ=255)            tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACg                                                                                                               <  2:827194/140‑1 (MQ=255)                tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGc                                                                                                           <  1:1004568/140‑1 (MQ=255)                tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGc                                                                                                           <  1:546862/140‑1 (MQ=255)                          tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATacaca                                                                                                 <  2:234521/140‑1 (MQ=255)                                      gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGTCGAACCCCCCCCACGTTGCCGTATACACACCTTCCCGGCgg                                                                                     >  1:609128/1‑139 (MQ=14)                                      gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGGTCGAACCCCCCCCACGTTGCCGTATAAACACCTTTCAggggg                                                                                     >  2:14360/1‑137 (MQ=14)                                       tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                                                                    <  1:14360/140‑1 (MQ=255)                                        aggggCTTCTCATCCCCCCGGTGGGTGCAATATAAGAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                                                                    <  1:1014992/139‑1 (MQ=17)                                                       ccccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCg                                                                    <  2:135522/140‑1 (MQ=255)                                                         ccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTCGAACCCCCCCAACGTTGCCCTGTACACACTTTCCCGGCGCGCTCCTTCCGCCCCCCggc                                                                  >  2:895998/1‑139 (MQ=17)                                                         ccGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACTCACTTTCCAGGCGTGCTCCTTCAGCCACTCGGa                                                                  <  1:610198/140‑1 (MQ=255)                                                              gtgtgCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                             <  1:25902/140‑1 (MQ=255)                                                                         aCG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGt                                                  <  1:911245/140‑1 (MQ=25)                                                                                                             cttTAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAgg               <  1:379136/140‑1 (MQ=255)                                                                                                                tAAATATGGCGGTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa            <  2:609128/140‑1 (MQ=255)                                                                                                                 aaaTATGGCGGTGAGGGGGGGGGGTCGAACCCCCCCTACGTTGCCGTATACACACTTTTCAGGCGGGGTCCTTCCGCCACCCGGACACCTCACCCAAATTTCGTTCCTGTCTTTCTGGGACGGGCGCTCATTTAGGGgaa           >  2:436227/1‑140 (MQ=12)                                                                                                                          ggTGAGGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCt  <  1:419668/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                       GTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCT  >  NC_000913/1097449‑1097707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑