Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,850	(C)6→7	coding (260/552 nt)	btuE ←	thioredoxin/glutathione peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,844	1	.	C	100.0%	164.3 / NA	46	V89G (GTG→GGG)	btuE	thioredoxin/glutathione peroxidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (25/21);  total (25/21)

ACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAAC  >  NC_000913/1793705‑1793975                                                                                                                                             |                                                                                                                                    ccGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCa                                                                                                                                      <  2:649425/139‑1 (MQ=255)   ggCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cc                                                                                                                                   <  2:276641/140‑1 (MQ=255)     ccTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cccc                                                                                                                                 <  1:607951/140‑1 (MQ=255)        ttGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑ccccccc                                                                                                                              >  2:491245/1‑139 (MQ=255)           cTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cccccccatg                                                                                                                           <  1:816288/140‑5 (MQ=255)           cTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑cccccccatg                                                                                                                           <  1:17932/140‑5 (MQ=255)             gACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCAtgtg                                                                                                                          <  1:491245/140‑1 (MQ=255)              aCCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtgg                                                                                                                         <  1:1065334/140‑1 (MQ=255)              aCCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtgg                                                                                                                         <  1:615883/140‑1 (MQ=255)               ccATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtggt                                                                                                                        >  1:228764/1‑140 (MQ=255)               ccATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATgtggt                                                                                                                        <  1:750152/140‑1 (MQ=255)                   aCGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTa                                                                                                                    >  2:568310/1‑140 (MQ=255)                       gCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGt                                                                                                                <  2:1269578/140‑1 (MQ=255)                        cATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTa                                                                                                               >  1:1167331/1‑140 (MQ=255)                                 cGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      <  2:640445/140‑1 (MQ=255)                                 cGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      >  2:804245/1‑140 (MQ=255)                                 cGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      >  1:1123595/1‑140 (MQ=255)                                 cGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      <  1:1376954/140‑1 (MQ=255)                                    tctctTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAtctc                                                                                                   >  2:274225/1‑140 (MQ=255)                                             gcgcGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCt                                                                                          >  2:1351708/1‑140 (MQ=255)                                                  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      >  2:374002/1‑123 (MQ=255)                                                  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAAt                                                                                                      <  1:374002/123‑1 (MQ=255)                                                  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTg                                                                                         <  2:675064/136‑1 (MQ=255)                                                  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTg                                                                                         >  1:675064/1‑136 (MQ=255)                                                  aCTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCg                                                                                     >  2:916575/1‑140 (MQ=255)                                                          cGGCGCTGCGGCAATCAATTTTTGATACAGCGCATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGt                                                                             <  2:1234223/140‑1 (MQ=255)                                                             cgcTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTcc                                                                          >  2:164842/1‑140 (MQ=255)                                                                          aatTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGc                                                             >  2:853297/1‑140 (MQ=255)                                                                             ttttGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACg                                                          <  2:774519/140‑1 (MQ=255)                                                                                             ggCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCAt                                          <  1:804245/140‑1 (MQ=255)                                                                                                gTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATaaa                                       >  1:1369358/1‑140 (MQ=255)                                                                                                    ttCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCt                                   >  1:803187/1‑140 (MQ=255)                                                                                                    ttCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCt                                   >  1:374052/1‑140 (MQ=255)                                                                                                    ttCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGCTGCTACATTAAGTCTTAATCTCTTCATCCCTGCACGGTTCTTGTTCCAGAAACTGCTTGCACGGGAATCCCAGCACCATAAAACCt                                   >  2:1329609/1‑140 (MQ=255)                                                                                                        ccATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                               <  2:803187/140‑1 (MQ=255)                                                                                                        ccATTAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                               <  2:374052/140‑1 (MQ=255)                                                                                                            tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                               >  1:1313244/1‑136 (MQ=255)                                                                                                            tAACTTCAATCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCtcgat                               <  2:1313244/136‑1 (MQ=255)                                                                                                                   aaTCTTACTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCt                    >  2:609302/1‑140 (MQ=255)                                                                                                                         aCTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAGCTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGa              >  2:435160/1‑140 (MQ=255)                                                                                                                         aCTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGa              >  1:300757/1‑140 (MQ=255)                                                                                                                         aCTGAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGa              >  1:1158346/1‑140 (MQ=255)                                                                                                                            gAACATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAata           <  1:1105464/140‑1 (MQ=255)                                                                                                                               cATCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtc        >  1:480908/1‑140 (MQ=255)                                                                                                                                 tCGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATtctc      >  1:457672/1‑140 (MQ=255)                                                                                                                                  cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCATCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc     >  2:351044/1‑140 (MQ=255)                                                                                                                                  cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa             <  2:1275738/132‑1 (MQ=255)                                                                                                                                  cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGaa             >  1:1275738/1‑132 (MQ=255)                                                                                                                                  cGGGAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTcc     >  1:866632/1‑140 (MQ=255)                                                                                                                                   gggAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCa    <  2:480908/140‑1 (MQ=255)                                                                                                                                   gggAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCa    <  2:1167331/140‑1 (MQ=255)                                                                                                                                    ggAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCaa   <  2:457672/140‑1 (MQ=255)                                                                                                                                     gAACGTCACCCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAAc  <  1:274225/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                    ACGGCCTTTGCTGACCATACGGGCATAGAATCCGCTCTCTTCCGGCGCGACTGCGGTCGGCGCTGCGGCAATCAATTTTTGATACAGCGGATGGCGTCCTTCGCCATTAACTTCAATCTTACTGAACATCGGGAACGTCA‑CCCCCCATGTGGTGGTACAGTAAGTTTTAATCTCTTCATCGCTGCCCGGTTCTTGTTCCAGAAACTGGTTGCACGGGAATCCCAGCACCATAAAACCTCGATCGACCCAGGCTTTCTGAATATTCTCCAAC  >  NC_000913/1793705‑1793975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑