Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,057,676	(C)7→8	intergenic (+102/+351)	yeeN → / ← asnW	putative transcriptional regulator YeeN/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,057,669	1	.	C	100.0%	105.1 / NA	30	intergenic (+95/+358)	yeeN/asnW	putative transcriptional regulator YeeN/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/14);  total (16/14)

ACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTC  >  NC_000913/2057531‑2057805                                                                                                                                            |                                                                                                                                         acgacgATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑c                                                                                                                                         <  1:72624/140‑1 (MQ=255)           aCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑ccccccccgtt                                                                                                                               <  1:64753/140‑5 (MQ=255)           aCAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑ccccccccgtt                                                                                                                               >  1:63994/1‑136 (MQ=255)             aaaaaGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTcc                                                                                                                              <  2:193550/140‑1 (MQ=255)                  gTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCagag                                                                                                                         >  2:699962/1‑140 (MQ=255)                               tCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAAtatttat                                                                                                            <  2:1263435/140‑1 (MQ=255)                                                 cTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTtcttct                                                                                          >  2:272920/1‑140 (MQ=255)                                                  ttttAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTcttctt                                                                                         >  2:75748/1‑140 (MQ=255)                                                     tAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCttttt                                                                                      <  2:99749/140‑1 (MQ=255)                                                       aaGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCt                                                                                    <  1:75748/140‑1 (MQ=255)                                                             tCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTAc                                                                              <  1:272920/140‑1 (MQ=255)                                                                    ttACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAATTTCCAGTTCTTCTTTTTTTGATTAAAGAAGGc                                                                       >  1:355002/1‑140 (MQ=255)                                                                           aGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTgg                                                                >  2:445265/1‑140 (MQ=255)                                                                           aGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTgg                                                                >  2:235744/1‑140 (MQ=255)                                                                              tttCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCaa                                                             <  2:843398/140‑1 (MQ=255)                                                                                       tctcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATa                                                    >  2:712033/1‑140 (MQ=255)                                                                                         tcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGt                                                  <  1:699962/140‑1 (MQ=255)                                                                                         tcATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGt                                                  <  2:1197841/140‑1 (MQ=255)                                                                                             ataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTcc                                              <  1:235744/140‑1 (MQ=255)                                                                                             ataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTcc                                              <  1:445265/140‑1 (MQ=255)                                                                                             ataATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTcc                                              <  1:237976/140‑1 (MQ=255)                                                                                                 ttCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGAt                                          >  2:92275/1‑140 (MQ=255)                                                                                                     tataAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATaaa                                       <  1:772992/139‑1 (MQ=255)                                                                                                     tataAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATaaa                                       >  2:772992/1‑139 (MQ=255)                                                                                                     tataAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAg                                      >  2:1033506/1‑140 (MQ=255)                                                                                                      ataAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGa                                     <  1:595865/140‑1 (MQ=255)                                                                                                      ataAAAAATATAATATTCAACTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGa                                     >  2:932730/1‑140 (MQ=255)                                                                                                                        aaCTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCg                   >  1:291093/1‑140 (MQ=255)                                                                                                                         aCTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGAc                         >  2:1254434/1‑133 (MQ=255)                                                                                                                         aCTCGTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGAc                         <  1:1254434/133‑1 (MQ=255)                                                                                                                             gTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATaa              >  1:34194/1‑140 (MQ=255)                                                                                                                             gTCATATTGATTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATaa              >  2:8716/1‑140 (MQ=255)                                                                                                                                      aTTATACCCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCAttttt     >  2:855338/1‑140 (MQ=255)                                                                                                                                        tata‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTtctc  >  2:445499/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                         ACGACGATGTACAAAAAGTTTATCATAACGTCGCAAATCTCTAATTATCTTTTAAAGAAATCTGTCTTTACGGCAGATTTCTTTAATCTCATATAATTCTTATAAAAAATATAATATTCAACTCGTCATATTGATTATA‑CCCCCCCGTTCCCAGAGAAATAATATTTATTAAAATTCCAGTTCTTCTTTTTCTGATTACAGAAGGCAAAGTGGCAATTACGCATAGTTTCCCGATAAAGACGCGATAGCGACATCCCGCATAAGGCATTTTTCTC  >  NC_000913/2057531‑2057805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑