Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,695,585	(G)7→8	intergenic (‑42/‑216)	purL ← / → mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,695,578	1	.	G	100.0%	222.5 / NA	61	intergenic (‑35/‑223)	purL/mltF	phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (32/29);  total (32/29)

GCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTT  >  NC_000913/2695440‑2695710                                                                                                                                            |                                                                                                                                     gCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑g                                                                                                                                     >  1:452532/1‑140 (MQ=255)  cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑gg                                                                                                                                    >  1:733218/1‑140 (MQ=255)  cGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑gg                                                                                                                                    <  1:555455/140‑1 (MQ=255)           tgtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑ggggggggaaa                                                                                                                           <  1:1175008/140‑5 (MQ=255)             tgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACg                                                                                                                          >  2:700041/1‑140 (MQ=255)               aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                        >  1:1299704/1‑140 (MQ=255)               aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                        >  2:676878/1‑140 (MQ=255)               aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                        >  2:176532/1‑140 (MQ=255)               aaCCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACggg                                                                                                                        <  1:676878/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  2:865637/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  1:800752/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  1:791449/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  2:733218/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  1:731943/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  1:227802/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  2:456741/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  2:19385/140‑1 (MQ=255)                 ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCACAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCg                                                                                                                      <  2:1299704/140‑1 (MQ=255)                   gggAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTc                                                                                                                    >  1:491449/1‑140 (MQ=255)                            gCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCGAAGGGGGGGGAAACGGGCGTCATTATAAAg                                                                                                           >  2:978065/1‑140 (MQ=255)                            gCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAg                                                                                                           >  2:612189/1‑140 (MQ=255)                            gCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAg                                                                                                           >  2:778074/1‑140 (MQ=255)                                  tGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTg                                                                                                     <  1:340265/140‑1 (MQ=255)                                          tGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTg                                                                                             >  1:141471/1‑140 (MQ=255)                                                 agTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAAc                                                                                      <  1:228359/140‑1 (MQ=255)                                                         gATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg                                                                              <  1:827278/140‑1 (MQ=255)                                                           ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg                                                                              >  1:786129/1‑138 (MQ=255)                                                           ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCg                                                                              <  2:786129/138‑1 (MQ=255)                                                           ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg                                                                            <  2:178681/140‑1 (MQ=255)                                                           ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg                                                                            >  1:1396170/1‑140 (MQ=255)                                                             cGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGa                                                                          >  1:398316/1‑140 (MQ=255)                                                              ggAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa                                                                         <  2:141471/140‑1 (MQ=255)                                                                aaTGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAAt                                                                       <  1:865677/140‑1 (MQ=255)                                                                     cGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt                                                                  <  2:1396170/140‑1 (MQ=255)                                                                      gACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATc                                                                 >  1:1133928/1‑140 (MQ=255)                                                                          gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCa                                                             >  2:1075890/1‑140 (MQ=255)                                                                             cAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg                                                          <  2:398316/140‑1 (MQ=255)                                                                              aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATaa                                                                     <  1:181950/128‑1 (MQ=255)                                                                              aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATaa                                                                     >  2:181950/1‑128 (MQ=255)                                                                                gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGCCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg                                                          >  1:1363176/1‑137 (MQ=255)                                                                                gCGAACCACGCAGAATTTCCATCATAAGTCTCTCGCCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg                                                          <  2:1363176/137‑1 (MQ=255)                                                                                   aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAtt                                                    >  2:98810/1‑140 (MQ=255)                                                                                   aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAtt                                                    >  1:12114/1‑140 (MQ=255)                                                                                     ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAg                                                  >  1:552296/1‑140 (MQ=255)                                                                                     ccACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAg                                                  >  1:1328311/1‑140 (MQ=255)                                                                                        cGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTg                                                        <  2:1395951/131‑1 (MQ=255)                                                                                        cGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTg                                                        >  1:1395951/1‑131 (MQ=255)                                                                                          cAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAAcg                                             >  1:595062/1‑140 (MQ=255)                                                                                              aTTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTg                                         <  1:700041/140‑1 (MQ=255)                                                                                                    atcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCa                                   <  2:1328311/140‑1 (MQ=255)                                                                                                       atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg                                >  2:1063522/1‑140 (MQ=255)                                                                                                          aGTCCCTCGTCTTCTAAGCTTTCGGTGTACCAAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCAC‑‑‑CGTGAATTAGCAACGCGTGCCGCCAATGGCTGta                          <  2:1359621/140‑1 (MQ=255)                                                                                                                tcGTCTTCTAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaata                       >  2:617434/1‑140 (MQ=255)                                                                                                                   tcttctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCa                                   <  2:1081314/125‑1 (MQ=255)                                                                                                                   tcttctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCa                                   >  1:1081314/1‑125 (MQ=255)                                                                                                                       ctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGTGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGcc                >  2:451253/1‑140 (MQ=255)                                                                                                                       ctAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGcc                >  2:777190/1‑140 (MQ=255)                                                                                                                        tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTg                  >  1:208557/1‑137 (MQ=255)                                                                                                                        tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTg                  <  2:208557/137‑1 (MQ=255)                                                                                                                        tAAGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCa               <  1:776358/140‑1 (MQ=255)                                                                                                                          aGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTTCCGCCAATGGCTGTAATAAGTTGCCATc             >  1:371711/1‑140 (MQ=255)                                                                                                                          aGCTTTCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATc             <  2:595062/140‑1 (MQ=255)                                                                                                                              ttCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgc         >  1:817946/1‑140 (MQ=255)                                                                                                                               tCGGTGTACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg        >  1:1259352/1‑140 (MQ=255)                                                                                                                                     tACCCAAGGGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGtt  >  2:393852/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                     GCGTAAATATTGTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAA‑GGGGGGGAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTT  >  NC_000913/2695440‑2695710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑