Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,130,215	C→T	G127G (GGC→GGT)	metL →	fused aspartate kinase/homoserine dehydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,130,215	0	C	T	100.0%	144.6 / NA	42	G127G (GGC→GGT)	metL	fused aspartate kinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/22);  total (20/22)

GTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAGGGCTT  >  NC_000913/4130080‑4130347                                                                                                                                        |                                                                                                                                     gTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACg                                                                                                                                  <  2:339947/140‑1 (MQ=255)   gCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACggg                                                                                                                                <  1:813516/140‑1 (MQ=255)                cGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCgg                                                                                                                   <  2:368655/140‑1 (MQ=255)                   tCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCAc                                                                                                                <  2:1215256/140‑1 (MQ=255)                     tGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGt                                                                                                              >  1:877737/1‑140 (MQ=255)                     tGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGt                                                                                                              >  1:1391898/1‑140 (MQ=255)                             gCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTc                                                                                                      >  2:1100910/1‑140 (MQ=255)                             gCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTc                                                                                                      >  1:1180811/1‑140 (MQ=255)                             gCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTc                                                                                                      >  2:252011/1‑140 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    <  2:133918/140‑1 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    <  2:1114203/140‑1 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    <  1:874079/140‑1 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    <  2:1277025/140‑1 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    <  2:1334558/140‑1 (MQ=255)                               tGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTg                                                                                                    >  2:333078/1‑140 (MQ=255)                                gaagaaGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGc                                                                                                   <  2:1203661/140‑1 (MQ=255)                                 aagaagCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCg                                                                                                  <  2:877737/140‑1 (MQ=255)                                      gCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACt                                                                                             <  2:745765/140‑1 (MQ=255)                                         gATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTaa                                                                                          <  2:223384/140‑1 (MQ=255)                                           tAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATc                                                                                        >  2:927470/1‑140 (MQ=255)                                                       cgcTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGc                                                                            >  1:874786/1‑140 (MQ=255)                                                         cTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCa                                                                          >  1:246908/1‑140 (MQ=255)                                                         cTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCa                                                                          <  1:252011/140‑1 (MQ=255)                                                          ttttGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAg                                                                         <  1:1353548/140‑1 (MQ=255)                                                          ttttGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAg                                                                         >  2:1353548/1‑140 (MQ=255)                                                            ttGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCg                                                                       >  1:1057391/1‑140 (MQ=255)                                                                                                aCAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGaa                                   <  1:333078/140‑1 (MQ=255)                                                                                                        aTTAACGACGCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAG‑TTTTACGCGCTGAACGCGCCGca                          <  1:1100910/140‑1 (MQ=255)                                                                                                              gacgCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGca                          >  1:1081975/1‑135 (MQ=255)                                                                                                              gacgCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGca                          <  2:1081975/135‑1 (MQ=255)                                                                                                                 gCAGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCa                  >  1:148838/1‑140 (MQ=255)                                                                                                                   aGTGTATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAgg                >  2:1259694/1‑140 (MQ=255)                                                                                                                     tgtATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGca                          <  1:890225/128‑1 (MQ=255)                                                                                                                     tgtATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGca                          >  2:890225/1‑128 (MQ=255)                                                                                                                     tgtATGCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGtt              >  2:1203362/1‑140 (MQ=255)                                                                                                                          gCGGAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTtgatga         >  2:86090/1‑140 (MQ=255)                                                                                                                             gAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAg       <  1:577109/139‑1 (MQ=255)                                                                                                                             gAAGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAg       >  2:577109/1‑139 (MQ=255)                                                                                                                              aaGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAggg     <  2:148838/140‑1 (MQ=255)                                                                                                                              aaGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAggg     <  1:1348338/140‑1 (MQ=255)                                                                                                                               aGTGGTGGGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAGGGc    >  1:649871/1‑140 (MQ=255)                                                                                                                                 tggtggGTCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAGGGCtt  >  1:1368263/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     GTGCGATCTGATTAGCGGTCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGACCTTGAGCGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGACGCAGTGTATGCGGAAGTGGTGGGCCACGGGGAAGTATGGTCGGCACGTCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAACGCGCCGCACAACCGCAGGTTGATGAAGGGCTT  >  NC_000913/4130080‑4130347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑