Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,132,292	T→C	intergenic (+25/‑324)	metL → / → metF	fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,132,292	0	T	C	100.0%	234.3 / NA	64	intergenic (+25/‑324)	metL/metF	fused aspartate kinase/homoserine dehydrogenase 2/5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/35);  total (29/35)

CTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTT  >  NC_000913/4132162‑4132425                                                                                                                                   |                                                                                                                                      cTGGTATCGCGATAACC‑‑CTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGtttt                                                                                                                            <  1:129877/140‑1 (MQ=255)    gTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTc                                                                                                                           >  1:1022599/1‑140 (MQ=255)           gATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTa                                                                                                                    <  1:1362829/140‑1 (MQ=255)            aTAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTAt                                                                                                                   <  2:289379/140‑1 (MQ=255)             tAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATg                                                                                                                  <  2:1022599/140‑1 (MQ=255)                 ccTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGttt                                                                                                              >  2:795431/1‑140 (MQ=255)                       gTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATg                                                                                                        <  1:212855/140‑1 (MQ=255)                           tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGt                                                                                                                <  2:367649/128‑1 (MQ=255)                           tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGt                                                                                                                >  1:367649/1‑128 (MQ=255)                           tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTa                                                                                                           >  2:533549/1‑133 (MQ=255)                           tCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTa                                                                                                           <  1:533549/133‑1 (MQ=255)                            ccGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAata                                                                                                   >  1:771229/1‑140 (MQ=255)                             cgcgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatat                                                                                                  >  2:3910/1‑140 (MQ=255)                              gcgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatata                                                                                                 <  2:544159/140‑1 (MQ=255)                              gcgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatata                                                                                                 <  1:1333556/140‑1 (MQ=255)                               gggACCTGGCGCTGGGCTCGACTTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAGatatat                                                                                                <  2:17422/139‑1 (MQ=255)                               cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                >  1:1125721/1‑140 (MQ=255)                               cgGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAatatat                                                                                                <  1:1159280/140‑1 (MQ=255)                                  aCCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTc                                                                                                             <  1:72875/124‑1 (MQ=255)                                  aCCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTc                                                                                                             >  2:72875/1‑124 (MQ=255)                                   ccTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGaa                                                                                            <  2:1266051/140‑1 (MQ=255)                                   ccTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGaa                                                                                            <  1:1375314/140‑1 (MQ=255)                                      ggCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTa                                                                                         >  1:1014337/1‑140 (MQ=255)                                         gcTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCa                                                                                      <  2:454718/140‑1 (MQ=255)                                              gcgcGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGa                                                                                 <  1:281284/140‑1 (MQ=255)                                                    cGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGc                                                                           <  1:172426/140‑1 (MQ=255)                                                       cACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCgg                                                                        >  1:807748/1‑140 (MQ=255)                                                            ccgGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTaa                                                                   >  2:1188177/1‑140 (MQ=255)                                                                ggCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGt                                                               >  2:127499/1‑140 (MQ=255)                                                                                atCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGc                                               <  1:1354904/140‑1 (MQ=255)                                                                                   aaCCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaag                                            <  1:635445/140‑1 (MQ=255)                                                                                   aaCCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaag                                            <  1:795431/140‑1 (MQ=255)                                                                                    aCCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaaga                                           >  1:1024775/1‑140 (MQ=255)                                                                                    aCCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCaaga                                           >  1:1267082/1‑140 (MQ=255)                                                                                        gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                       >  1:841294/1‑140 (MQ=255)                                                                                        gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                       >  1:507706/1‑140 (MQ=255)                                                                                        gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                       >  2:156680/1‑140 (MQ=255)                                                                                        gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                       >  2:1132613/1‑140 (MQ=255)                                                                                        gCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCa                                       >  2:1136601/1‑140 (MQ=255)                                                                                          tGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     <  2:841294/140‑1 (MQ=255)                                                                                          tGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     <  2:519559/140‑1 (MQ=255)                                                                                          tGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     >  2:1042471/1‑140 (MQ=255)                                                                                          tGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     <  1:557189/140‑1 (MQ=255)                                                                                               caGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTa                                <  1:518926/140‑1 (MQ=255)                                                                                                  ttgttgTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     <  2:1080204/132‑1 (MQ=255)                                                                                                  ttgttgTAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     >  1:1080204/1‑132 (MQ=255)                                                                                                   tgttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATg                            <  2:507706/140‑1 (MQ=255)                                                                                                    gttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATgg                           <  2:807748/140‑1 (MQ=255)                                                                                                    gttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATgg                           <  1:127499/140‑1 (MQ=255)                                                                                                    gttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATgg                           >  2:399296/1‑140 (MQ=255)                                                                                                     ttgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGt                          <  2:1024775/140‑1 (MQ=255)                                                                                                      tgtAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGtt                         >  1:503422/1‑140 (MQ=255)                                                                                                        tAATTTCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAg                       <  1:399296/140‑1 (MQ=255)                                                                                                            ttCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     <  1:1205806/122‑1 (MQ=255)                                                                                                            ttCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAAt                                     >  2:1205806/1‑122 (MQ=255)                                                                                                            ttCAGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTta                   >  2:386059/1‑140 (MQ=255)                                                                                                               aGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAg                       >  2:7557/1‑133 (MQ=255)                                                                                                               aGAAATTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAg                       <  1:7557/133‑1 (MQ=255)                                                                                                                  aaTTTAATAATGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTg             >  2:175770/1‑140 (MQ=255)                                                                                                                           atGCCCTGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGt    <  2:835262/140‑1 (MQ=255)                                                                                                                           atGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGt    <  1:1132613/140‑1 (MQ=255)                                                                                                                           atGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGt    <  1:1042471/140‑1 (MQ=255)                                                                                                                            tGCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGtt   >  2:431115/1‑140 (MQ=255)                                                                                                                             gCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGttt  >  2:504652/1‑140 (MQ=255)                                                                                                                             gCCCGGCACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGttt  <  1:670552/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      CTGGTATCGCGATAACCCTCTGGTGATCCGCGGACCTGGCGCTGGGCGCGACGTCACCGCCGGGGCGATTCAGTCGGATATCAACCGGCTGGCACAGTTGTTGTAATTTCAGAAATTTAATAATGCCCGGTACTCATGTTTTCGGGTTTATGGTTTCTAATGAAATATATTGAATTATCATAGGATTAGGCCGGATTAAGCGTTTACGACGAATCCGGCAAGAAGCAATAAGTACATGGTTAGTTTATATTTGCAGTCCGGTTT  >  NC_000913/4132162‑4132425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑